| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Not applicable (research chemical). Nicotinohydrazide does not have a well-defined pharmacological target. It is a small molecule compound used as a research chemical and chemical intermediate rather than as a therapeutic agent.
|
|---|---|
| ln Vitro |
Nicotinohydrazide is a small molecule compound used as a research chemical. It does not exhibit direct pharmacological activity in standard in vitro assays. Its applications are focused on chemical research and as a synthetic intermediate.
|
| ln Vivo |
In vivo studies of nicotinohydrazide are not applicable as the compound is a research chemical rather than a pharmacologically active compound.
|
| Enzyme Assay |
Non-cell-based assays for nicotinohydrazide are analytical in nature. Standard methods including HPLC, NMR, and mass spectrometry are used for identification, quantification, and purity assessment. Physicochemical properties such as melting point are determined using standard protocols.
|
| Cell Assay |
Cell-based assays for nicotinohydrazide are not commonly performed, as the compound is a research chemical rather than a pharmacologically active compound.
|
| Animal Protocol |
In vivo animal studies for nicotinohydrazide are not applicable as the compound is a research chemical.
|
| ADME/Pharmacokinetics |
Nicotinohydrazide has a molecular formula of C₆H₇N₃O and a molecular weight of 137.14 g/mol. It appears as a white to almost white powder or crystal with a melting point of 160.0 to 164.0 °C. The compound is a derivative of nicotinic acid and should be stored under inert gas.
|
| Toxicity/Toxicokinetics |
Specific toxicity data for nicotinohydrazide are limited. Standard laboratory safety practices should be followed when handling this compound, including the use of personal protective equipment.
|
| References |
[1]. Kruszynski R. A structural and theoretical study of intermolecular interactions in nicotinohydrazide dihydrochloride. Acta Crystallogr C. 2011 Feb;67(Pt 2):o52-6.
|
| Additional Infomation |
Structural homologues of isoniazid; structures are described in the first reference.
Nicotinohydrazide (Nicotinic acid hydrazide) is a small molecule compound and a derivative of nicotinic acid. It is used as a research chemical and chemical intermediate. The compound is for research use only. |
| Molecular Formula |
C6H7N3O
|
|---|---|
| Molecular Weight |
137.14
|
| Exact Mass |
137.058
|
| CAS # |
553-53-7
|
| PubChem CID |
11112
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.2±0.1 g/cm3
|
| Melting Point |
159-161 °C(lit.)
|
| Index of Refraction |
1.584
|
| LogP |
-0.83
|
| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
1
|
| Heavy Atom Count |
10
|
| Complexity |
126
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
NNC(C1=CN=CC=C1)=O
|
| InChi Key |
KFUSANSHCADHNJ-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C6H7N3O/c7-9-6(10)5-2-1-3-8-4-5/h1-4H,7H2,(H,9,10)
|
| Chemical Name |
pyridine-3-carbohydrazide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 7.2918 mL | 36.4591 mL | 72.9182 mL | |
| 5 mM | 1.4584 mL | 7.2918 mL | 14.5836 mL | |
| 10 mM | 0.7292 mL | 3.6459 mL | 7.2918 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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