| Size | Price | Stock | Qty |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
Thiol groups (sulfhydryl groups) in biomolecules. 2-Maleimido acetic acid is a protein cross-linking agent. The maleimide group in the compound reacts selectively with thiol groups in biomolecules to form a covalent bond. This enables the connection of biomolecules with thiol groups, facilitating bioconjugation and protein modification. The compound does not have a specific pharmacological target but serves as a chemical tool for protein chemistry.
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| ln Vitro |
2-Maleimido acetic acid is a protein cross-linking agent that reacts selectively with thiol groups to form stable thioether bonds. It is widely utilized in bioconjugation to link proteins, peptides, and polymers. The compound is used in the preparation of trivalent Gd-DOTA reagents for protein modification. Its in vitro activity includes site-specific conjugation of biomolecules for research applications.
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| ln Vivo |
In vivo studies of 2-maleimido acetic acid are not applicable as the compound is a chemical reagent used for in vitro bioconjugation and protein modification. It is not administered as a therapeutic agent. The compound is used exclusively in biochemical research applications.
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| Enzyme Assay |
Non-cell-based assays for 2-maleimido acetic acid include characterization of its thiol reactivity using model thiol compounds (e.g., cysteine, glutathione). The reaction is monitored by HPLC, mass spectrometry, or colorimetric assays (e.g., Ellman’s assay for free thiol quantification). The compound’s ability to cross-link proteins is assessed by SDS-PAGE or mass spectrometry.
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| Cell Assay |
Cell-based assays for 2-maleimido acetic acid are not commonly performed, as the compound is a chemical reagent used for protein modification rather than a pharmacologically active compound. Proteins or peptides modified with the compound may be tested in cell-based assays.
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| Animal Protocol |
In vivo animal studies for 2-maleimido acetic acid are not applicable as the compound is a chemical reagent used for in vitro research applications.
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| ADME/Pharmacokinetics |
2-Maleimido acetic acid has a molecular formula of C₆H₅NO₄ and a molecular weight of 155.11 g/mol. It appears as an off-white to white crystal powder. Purity is ≥100% (by HPLC). The compound is a carboxylic acid bearing a maleimide functional group. It should be stored under recommended conditions. It is intended for research use only.
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| Toxicity/Toxicokinetics |
2-Maleimido acetic acid is a protein cross-linking agent and is generally considered to have low toxicity for research use. Standard laboratory safety practices should be followed when handling this compound.
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| References | |
| Additional Infomation |
2-Maleimido acetic acid (N-Maleimidylglycine) is a protein cross-linking agent and bioconjugation reagent with a maleimide functional group. The maleimide group reacts selectively with thiol groups in biomolecules to form stable thioether bonds, enabling site-specific conjugation of proteins, peptides, and polymers. It is widely utilized in biochemical research for bioconjugation, protein modification, and drug delivery applications. It is for research use only.
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| Molecular Formula |
C6H5NO4
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|---|---|
| Molecular Weight |
155.11
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| Exact Mass |
155.021
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| CAS # |
25021-08-3
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| PubChem CID |
319935
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| Appearance |
White to off-white solid powder
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
376.7±25.0 °C at 760 mmHg
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| Melting Point |
116°C
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| Flash Point |
181.6±23.2 °C
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| Vapour Pressure |
0.0±1.8 mmHg at 25°C
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| Index of Refraction |
1.581
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| LogP |
-0.69
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
11
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| Complexity |
237
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=C1C([H])=C([H])C(N1C([H])([H])C(=O)O[H])=O
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| InChi Key |
GBKPNGVKZQBPCZ-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H5NO4/c8-4-1-2-5(9)7(4)3-6(10)11/h1-2H,3H2,(H,10,11)
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| Chemical Name |
2-(2,5-dioxopyrrol-1-yl)acetic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.4470 mL | 32.2352 mL | 64.4704 mL | |
| 5 mM | 1.2894 mL | 6.4470 mL | 12.8941 mL | |
| 10 mM | 0.6447 mL | 3.2235 mL | 6.4470 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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