| Size | Price | |
|---|---|---|
| Other Sizes |
| Targets |
Acetylcholinesterase (AChE). 4-Methylsyringol is a cholinesterase inhibitor that inhibits the action of acetylcholinesterase, the enzyme responsible for breaking down acetylcholine. Acetylcholine is a neurotransmitter important for cognitive function and muscle control. By inhibiting AChE, the compound increases acetylcholine levels, which can affect nerve signal transmission. Its potential use as an insecticide is based on this mechanism.
|
|---|---|
| ln Vitro |
4-Methylsyringol acts as a cholinesterase inhibitor, inhibiting acetylcholinesterase activity. As a natural product isolated from hardwood and Pinus yunnanensis, it has potential use as an insecticide. Its specific in vitro activities depend on the concentrations tested and the assay systems used. The compound’s fragrance properties also make it of interest for aroma research.
|
| ln Vivo |
In vivo studies of 4-methylsyringol are limited. As a cholinesterase inhibitor with potential use as an insecticide, it may have effects on the nervous system of insects and other organisms. The compound is a natural product from hardwood and Pinus yunnanensis. Further in vivo studies are needed to characterize its pharmacokinetic and pharmacodynamic properties, as well as its efficacy and safety as an insecticide.
|
| Enzyme Assay |
Non-cell-based assays for 4-methylsyringol include acetylcholinesterase inhibition assays using purified enzyme. The enzyme is incubated with acetylcholine substrate in the presence of various concentrations of the compound, and enzyme activity is measured by detecting the hydrolysis product using Ellman’s method or similar assays. The IC₅₀ value is calculated from the dose-response curve. Standard analytical methods including HPLC, NMR, and mass spectrometry are used for compound characterization.
|
| Cell Assay |
Cell-based assays for 4-methylsyringol use neuronal cell lines to assess acetylcholinesterase inhibition and its effects on neuronal function. Cells are treated with the compound at various concentrations, and acetylcholinesterase activity in cell lysates is measured. The compound’s effects on acetylcholine levels and neuronal signaling can be assessed. Cytotoxicity is assessed using standard cell viability assays.
|
| Animal Protocol |
In vivo studies of 4-methylsyringol are limited. Based on its acetylcholinesterase inhibitory activity, potential animal models include: insect models for evaluating insecticidal activity; and rodent models for studying effects on the nervous system. Standard protocols for these models involve administration of the compound followed by measurement of relevant endpoints.
|
| ADME/Pharmacokinetics |
4-Methylsyringol has a molecular formula of C₉H₁₂O₃ and a molecular weight of 168.19 g/mol. Purity is ≥99.63%. It is a natural product isolated from hardwood and Pinus yunnanensis. The compound is a phenolic fragrance type with a medicinal odor. It should be stored under recommended conditions. It is intended for research use only.
|
| Toxicity/Toxicokinetics |
Specific toxicity data for 4-methylsyringol are limited. As a cholinesterase inhibitor, it may have effects on the nervous system at high doses. Standard laboratory safety practices should be followed when handling this compound.
|
| References | |
| Additional Infomation |
2,6-Dimethoxy-4-methylphenol is a member of the methoxybenzene class and the phenol class.
4-Methylsyringol (2,6-dimethoxy-4-methylphenol) is a natural product isolated from hardwood and Pinus yunnanensis. It has the molecular formula C₉H₁₂O₃ and a molecular weight of 168.19 g/mol. The compound acts as a cholinesterase inhibitor, inhibiting acetylcholinesterase, and has potential use as an insecticide. It is a phenolic fragrance type with a medicinal odor and is intended for research use only. |
| Molecular Formula |
C9H12O3
|
|---|---|
| Molecular Weight |
168.19
|
| Exact Mass |
168.079
|
| CAS # |
6638-05-7
|
| PubChem CID |
240925
|
| Appearance |
Colorless to light yellow liquid
|
| Density |
1.105g/cm3
|
| Boiling Point |
145-146 °C14 mm Hg(lit.)
|
| Melting Point |
36 °C
|
| Flash Point |
>230 °F
|
| Index of Refraction |
1.52
|
| LogP |
1.717
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
12
|
| Complexity |
124
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
O(C([H])([H])[H])C1C(=C(C([H])=C(C([H])([H])[H])C=1[H])OC([H])([H])[H])O[H]
|
| InChi Key |
ZFBNNSOJNZBLLS-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C9H12O3/c1-6-4-7(11-2)9(10)8(5-6)12-3/h4-5,10H,1-3H3
|
| Chemical Name |
2,6-dimethoxy-4-methylphenol
|
| Synonyms |
4-Methylsyringol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 100 mg/mL (594.57 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 5.9457 mL | 29.7283 mL | 59.4566 mL | |
| 5 mM | 1.1891 mL | 5.9457 mL | 11.8913 mL | |
| 10 mM | 0.5946 mL | 2.9728 mL | 5.9457 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
