| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Unknown (alkaloid with potential enzyme or receptor targets). Rhombifoline is an alkaloid that acts by interacting with specific biological pathways, which may include modulating enzyme activity or binding to receptors. As a quinolizidine alkaloid, it may interact with neurotransmitter receptors or ion channels, similar to other alkaloids in this class. Its antioxidant activity suggests interactions with oxidative stress pathways. However, specific molecular targets have not been fully characterized.
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|---|---|
| ln Vitro |
Rhombifoline exhibits antioxidant activity. As an alkaloid, it may have additional biological activities including potential effects on the nervous system or other physiological pathways. Its specific in vitro activities depend on the concentrations tested and the assay systems used. Further studies are needed to fully characterize its activity profile, including potential enzyme inhibition or receptor binding activities.
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| ln Vivo |
In vivo studies of rhombifoline are limited. As an alkaloid isolated from Anagyrus foetida and Genista tenera, it may contribute to the pharmacological effects of these plants. Quinolizidine alkaloids are known for their diverse biological activities, including effects on the nervous system. Further in vivo studies are needed to characterize its pharmacokinetic and pharmacodynamic properties, as well as its efficacy and safety profile.
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| Enzyme Assay |
Non-cell-based assays for rhombifoline include enzyme inhibition studies to assess its effects on potential targets. Antioxidant activity is assessed using DPPH radical scavenging, ABTS, or FRAP assays. Receptor binding assays may be performed using radiolabeled ligands for neurotransmitter receptors or ion channels. Standard analytical methods including HPLC, NMR, and mass spectrometry are used for compound characterization and purity assessment.
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| Cell Assay |
Cell-based assays for rhombifoline use various cell lines to assess its biological activities. For antioxidant studies, cells are exposed to oxidative stress inducers and treated with the compound, and intracellular ROS levels are measured. For potential neuroactive effects, neuronal cell lines can be used to study effects on neuronal excitability and neurotransmitter release. Cytotoxicity is assessed using standard cell viability assays.
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| Animal Protocol |
In vivo studies of rhombifoline are limited. Based on its alkaloid nature, potential animal models include: models for studying neurological effects; models for evaluating antioxidant activity; and models for assessing anti-inflammatory or other pharmacological effects. Standard protocols for these models involve administration of the compound followed by measurement of relevant endpoints.
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| ADME/Pharmacokinetics |
Rhombifoline has a molecular formula of C₁₅H₂₀N₂O and a molecular weight of 244.33 g/mol. The SMILES notation is provided. It is a quinolizidine alkaloid isolated from Anagyrus foetida and Genista tenera. The compound should be stored under recommended conditions. It is intended for research use only.
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| Toxicity/Toxicokinetics |
Specific toxicity data for rhombifoline are limited. As an alkaloid, it may have biological activity at certain concentrations. Quinolizidine alkaloids are known to have diverse pharmacological effects and may be toxic at high doses. Standard laboratory safety practices should be followed when handling this compound.
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| References | |
| Additional Infomation |
According to reports, spatholobinin has been found in Laburnum anagyroides, Sophora franchetiana, and other organisms with available data.
Rhombifoline is a naturally occurring quinolizidine alkaloid first isolated from the leaves and stems of Anagyrus foetida L. and also found in Genista tenera. It has the molecular formula C₁₅H₂₀N₂O and a molecular weight of 244.33 g/mol. The compound exhibits antioxidant activity and acts by interacting with specific biological pathways, which may include modulating enzyme activity or binding to receptors. It is used as a research tool for studying alkaloid pharmacology and natural product chemistry. It is for research use only. |
| Molecular Formula |
C15H20N2O
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|---|---|
| Molecular Weight |
244.33
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| Exact Mass |
244.158
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| CAS # |
529-78-2
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| PubChem CID |
92795
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| Appearance |
Light yellow to light brown liquid
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| Density |
1.14±0.1 g/cm3 at 760 mmHg
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| Boiling Point |
120 °C
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| LogP |
1.781
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
2
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
18
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| Complexity |
424
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| Defined Atom Stereocenter Count |
2
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| SMILES |
C=CCCN1CC2CC(C1)C3=CC=CC(=O)N3C2
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| InChi Key |
ZVTFRRVBMAUIQW-QWHCGFSZSA-N
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| InChi Code |
InChI=1S/C15H20N2O/c1-2-3-7-16-9-12-8-13(11-16)14-5-4-6-15(18)17(14)10-12/h2,4-6,12-13H,1,3,7-11H2/t12-,13+/m0/s1
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| Chemical Name |
(1R,9S)-11-but-3-enyl-7,11-diazatricyclo[7.3.1.02,7]trideca-2,4-dien-6-one
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| Synonyms |
Rhombifoline
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (409.28 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.0928 mL | 20.4641 mL | 40.9283 mL | |
| 5 mM | 0.8186 mL | 4.0928 mL | 8.1857 mL | |
| 10 mM | 0.4093 mL | 2.0464 mL | 4.0928 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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