| Size | Price | Stock | Qty |
|---|---|---|---|
| 10mg |
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| Other Sizes |
| Targets |
Proteoglycan synthesis; ribozyme GlmS activator. Glucosamine 6-sulfate is a naturally occurring compound that serves as a precursor for proteoglycan formation, playing a pivotal role in conferring strength and elasticity to cartilage. It is also a ribozyme GlmS activator. It plays a variety of catalytic roles in glmS ribozymes.
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|---|---|
| ln Vitro |
Glucosamine 6-sulfate is a naturally occurring compound that plays a vital role in the structure and functionality of joint cartilage. It serves as a precursor for proteoglycan formation and is involved in collagen synthesis and breakdown. It is also a ribozyme GlmS activator and plays catalytic roles in glmS ribozymes.
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| ln Vivo |
In vivo, glucosamine 6-sulfate is a naturally occurring compound present in the body that plays a vital role in joint cartilage structure and function. It is commonly used in the treatment of osteoarthritis, as it promotes cartilage repair and reduces inflammation by stimulating the production of proteoglycans and hyaluronic acid. It is explored as a therapeutic option in managing conditions such as osteoarthritis and metabolic syndrome.
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| Enzyme Assay |
Non-cell-based assays for glucosamine 6-sulfate include its use as a substrate or activator in biochemical assays. GlmS ribozyme activity assays measure the compound's ability to activate the glmS ribozyme. Standard analytical methods including HPLC, NMR, and mass spectrometry are used for compound characterization and purity assessment. Physicochemical properties such as molecular weight (259.23 g/mol) and molecular formula (C₆H₁₃NO₈S) are determined using standard protocols.
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| Cell Assay |
Cell-based assays for glucosamine 6-sulfate use chondrocytes or other cartilage-related cell lines to assess its effects on proteoglycan synthesis, collagen production, and inflammation. Cells are treated with the compound at various concentrations, and proteoglycan production is measured using biochemical assays. Inflammatory cytokine production is assessed in macrophage or chondrocyte cell lines. Cytotoxicity is assessed using standard cell viability assays.
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| Animal Protocol |
In vivo studies of glucosamine 6-sulfate are conducted in animal models of osteoarthritis and other joint disorders. The compound is administered via oral or intraperitoneal injection. Cartilage repair, proteoglycan content, inflammatory markers, and joint function are assessed. Pharmacokinetic parameters are determined from blood and tissue samples. The compound is studied for its therapeutic potential in osteoarthritis and metabolic syndrome.
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| ADME/Pharmacokinetics |
Glucosamine 6-sulfate has a molecular formula of C₆H₁₃NO₈S and a molecular weight of 259.23 g/mol. It appears as a white to off-white solid powder. Purity is ≥95%. Storage: 4°C, sealed storage, away from moisture. It is a naturally occurring amino sugar derivative belonging to the glycosaminoglycan (GAG) class.
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| Toxicity/Toxicokinetics |
Glucosamine 6-sulfate is a naturally occurring compound that is generally considered to have low toxicity. It is commonly used in the treatment of osteoarthritis. Standard laboratory safety practices should be followed when handling this compound. It is intended for research use only.
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| References | |
| Additional Infomation |
Glucosamine 6-sulfate (GlcN6S) is a naturally occurring amino sugar derivative belonging to the glycosaminoglycan (GAG) class. It plays a vital role in the structure and functionality of joint cartilage as a component of proteoglycans. It is commonly used in the treatment of osteoarthritis, promoting cartilage repair and reducing inflammation. It is also a ribozyme GlmS activator and plays catalytic roles in glmS ribozymes. It is for research use only.
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| Molecular Formula |
C6H13NO8S
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|---|---|
| Molecular Weight |
259.23
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| Exact Mass |
259.036
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| CAS # |
91674-26-9
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| PubChem CID |
18634851
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| Appearance |
White to off-white solid powder
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| Density |
1.757g/cm3
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| Melting Point |
60-162 ºC
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| Index of Refraction |
1.599
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| LogP |
-6.4
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
16
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| Complexity |
311
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| Defined Atom Stereocenter Count |
4
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| SMILES |
[C@@H](O)([C@H](O)COS(O)(=O)=O)[C@H](O)[C@@H](N)C=O
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| InChi Key |
KRCSEEITTBNSQS-SLPGGIOYSA-N
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| InChi Code |
InChI=1S/C6H13NO8S/c7-3(1-8)5(10)6(11)4(9)2-15-16(12,13)14/h1,3-6,9-11H,2,7H2,(H,12,13,14)/t3-,4+,5+,6+/m0/s1
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| Chemical Name |
[(2R,3S,4R,5R)-5-amino-2,3,4-trihydroxy-6-oxohexyl] hydrogen sulfate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 250 mg/mL (964.39 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.8576 mL | 19.2879 mL | 38.5758 mL | |
| 5 mM | 0.7715 mL | 3.8576 mL | 7.7152 mL | |
| 10 mM | 0.3858 mL | 1.9288 mL | 3.8576 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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