| Size | Price | Stock | Qty |
|---|---|---|---|
| 40KU |
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| 80KU |
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| Targets |
Deoxyribonuclease II targets DNA, specifically hydrolyzing the phosphodiester bonds of deoxyribonucleotides in both native and denatured DNA. The enzyme acts as an endonuclease, cleaving internal phosphodiester bonds to produce oligonucleotides and nucleotides with 3'-phosphate and 5'-hydroxyl termini. DNase II is a lysosomal enzyme that plays a role in the degradation of exogenous DNA and in cellular DNA metabolism. It is active under acidic conditions (optimal pH approximately 4.6-5.0) and requires divalent metal ions for activity.
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| ln Vitro |
In vitro, Deoxyribonuclease II hydrolyzes the phosphodiester bonds of DNA, producing 3'-phosphate and 5'-hydroxyl termini. The enzyme is active on both native and denatured DNA. DNase II also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. The enzyme's activity is measured by the release of acid-soluble nucleotides from DNA substrates. DNase II is used in molecular biology applications including nucleic acid digestion, chromatin research, and studies of intracellular DNA breakdown pathways. The enzyme is also used to remove DNA from protein preparations.
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| ln Vivo |
In vivo, Deoxyribonuclease II is a lysosomal enzyme involved in the degradation of DNA. It plays a role in the clearance of apoptotic cell debris, the degradation of exogenous DNA, and the turnover of cellular DNA. DNase II deficiency in animal models leads to accumulation of undigested DNA in lysosomes and is associated with inflammatory and autoimmune phenotypes. The enzyme's activity in vivo is essential for maintaining genomic stability and preventing immune activation by self-DNA.
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| Enzyme Assay |
Non-cell-based assays for Deoxyribonuclease II involve measuring its enzymatic activity using DNA substrates. The enzyme is incubated with DNA (e.g., calf thymus DNA) under acidic conditions (pH 4.6-5.0), and the hydrolysis of DNA is measured. Activity is quantified by the release of acid-soluble nucleotides (A₂₆₀ measurement) or by fluorescence-based assays using labeled DNA substrates. The enzyme's activity is expressed in units per mg protein, with typical activities of ≥1000-12000 units/mg depending on the source and purity. The enzyme's properties including molecular weight (~38 kDa) and optimal pH are characterized.
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| Cell Assay |
DNase II is not used in cell-based assays as a biologically active compound but as an enzyme for nucleic acid digestion. In cell biology research, DNase II may be used to study DNA degradation pathways in lysosomes or to prepare DNA-free samples for downstream applications. Cells may be treated with DNase II inhibitors to study the role of DNase II in DNA metabolism and immune responses. The enzyme is not directly added to cell cultures as a therapeutic agent.
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| Animal Protocol |
Deoxyribonuclease II is not used in animal experiments as a therapeutic agent. It is an enzyme used in research applications. In animal studies, DNase II knockout models are used to study the role of the enzyme in DNA degradation, inflammation, and autoimmune diseases. The enzyme's activity may be measured in tissues or biological fluids to assess lysosomal function or DNA degradation capacity. However, the enzyme itself is not administered to animals as a therapeutic.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to Deoxyribonuclease II as it is not a therapeutic agent. The enzyme is a laboratory reagent used for nucleic acid digestion. Its activity, stability, and optimal reaction conditions are characterized for in vitro applications. The enzyme is typically supplied as a lyophilized powder or solution and stored at appropriate conditions to maintain activity. No PK data are available or relevant.
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| Toxicity/Toxicokinetics |
The toxicity of Deoxyribonuclease II has not been evaluated as it is not a therapeutic agent. The enzyme is a laboratory reagent handled with standard laboratory safety precautions. As a protein enzyme, it may cause allergic reactions in sensitive individuals. The enzyme is for research use only and is not intended for human consumption or therapeutic use. Safety data sheets recommend standard protective measures including gloves and eye protection when handling the enzyme.
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| References | |
| Additional Infomation |
Deoxyribonuclease II (CAS# 9025-64-3) is an endonuclease that hydrolyzes the phosphodiester bonds of deoxyribonucleotides in native and denatured DNA, producing 3'-phosphate and 5'-hydroxyl termini. It is a nucleic acid endonuclease primarily located within lysosomes that cleaves DNA between the 5'-phosphate and 3'-hydroxyl groups, degrading exogenous DNA and generating nucleoside-3'-phosphate products. DNase II is also known as Acid DNase and is used in molecular biology research for nucleic acid digestion, chromatin studies, and investigation of intracellular DNA breakdown pathways. The enzyme is available as a research reagent and is not approved as a therapeutic agent.
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| Molecular Formula |
232-801-8
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|---|---|
| Molecular Weight |
号MFCD00130919
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| CAS # |
9025-64-3
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| Appearance |
White to light yellow solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: ≥ 5 mg/mL
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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