| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
H-Met-Met-OH does not have a specific pharmacological target but is studied as a dipeptide involved in methionine metabolism and peptide transport. Methionine is an essential sulfur-containing amino acid involved in protein synthesis, methylation reactions, and antioxidant defense (via glutathione synthesis). The dipeptide form may have different transport properties and bioavailability compared to free methionine. H-Met-Met-OH may interact with peptide transporters (PEPT1, PEPT2) for intestinal absorption and cellular uptake, and may be hydrolyzed by intracellular peptidases to release free methionine.
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| ln Vitro |
In vitro, H-Met-Met-OH has been studied for its effects on cell function and metabolism. It has been observed that methionyl-methionine promotes α-s1 casein synthesis in bovine mammary gland explants. The dipeptide may modulate protein synthesis and nitrogen metabolism. H-Met-Met-OH has been shown to have various health benefits in animals, including effects on growth, immune function, and antioxidant status. The compound's activity is concentration-dependent and may involve signaling pathways related to amino acid sensing (mTOR).
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| ln Vivo |
In vivo, H-Met-Met-OH is orally active and has been studied as a dietary supplement in animals. It has been shown to have various health benefits including promoting growth, improving immune function, and enhancing antioxidant defense. The dipeptide form may provide advantages over free methionine in terms of bioavailability, stability, or targeted delivery. In livestock and poultry, methionine dipeptides are used as feed additives. However, specific in vivo data for H-Met-Met-OH are limited.
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| Enzyme Assay |
Non-cell-based assays for H-Met-Met-OH involve characterization of its chemical properties, purity, and stability. Analytical methods including HPLC, LC-MS, and NMR are used to confirm identity and purity. The compound's solubility, stability in various buffers, and degradation products are characterized. In enzyme assays, the compound may be used as a substrate for peptidases to study dipeptide hydrolysis kinetics. Peptidase activity is measured by detecting free methionine or by monitoring substrate disappearance.
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| Cell Assay |
Cellular assays for H-Met-Met-OH are performed using various cell lines, including mammary epithelial cells, intestinal cells, or immune cells. Cells are treated with H-Met-Met-OH at various concentrations, and cellular responses are measured. Protein synthesis is assessed by ³H-leucine or ³H-methionine incorporation. Cell proliferation is measured by MTT or CellTiter-Glo assays. Signaling pathways including mTOR and amino acid sensing are evaluated by Western blot. Methionine metabolism and glutathione levels are measured. The dipeptide's effects on cell function and metabolism are compared to free methionine.
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| Animal Protocol |
In vivo experiments with H-Met-Met-OH are conducted in animal models including rodents, livestock, and poultry. Animals are administered H-Met-Met-OH via oral gavage, dietary supplementation, or injection. Growth performance, feed intake, body weight gain, and feed conversion ratio are measured. Immune function is assessed by antibody titers, cytokine production, and immune cell populations. Antioxidant status is evaluated by measuring glutathione levels, antioxidant enzyme activities, and oxidative stress markers. Methionine metabolism and protein synthesis are assessed in tissues.
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| ADME/Pharmacokinetics |
H-Met-Met-OH is orally active and demonstrates favorable pharmacokinetic properties as a dipeptide. Following oral administration, the dipeptide is absorbed through peptide transporters (PEPT1) in the intestine and enters the bloodstream. It may be hydrolyzed by peptidases to release free methionine, which then enters methionine metabolism pathways. The compound's bioavailability, systemic exposure, and metabolic fate have been characterized in animal studies. The dipeptide form may provide advantages over free methionine in terms of stability and absorption kinetics.
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| Toxicity/Toxicokinetics |
The toxicity profile of H-Met-Met-OH has been evaluated in the context of its use as a dietary supplement. Methionine is an essential amino acid, and at physiological doses, the dipeptide is considered safe and well-tolerated. At high doses, methionine can be toxic, and the same may apply to the dipeptide. Potential toxicities include hypermethioninemia, oxidative stress, and neurotoxicity at excessive doses. In animal studies, H-Met-Met-OH has been shown to be safe at dietary supplementation levels. Comprehensive toxicological studies are limited.
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| References |
[1]. Lis MT, et, al. Effect of dietary changes on intestinal absorption of L-methionine and L-methionyl-L-methionine in the rat. Br J Nutr. 1972 Jan;27(1):159-67.
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| Additional Infomation |
Met-Met is a dipeptide formed from two L-methionine residues. It is a metabolite of Mycoplasma genitalium. Functionally, it is related to L-methionine.
See also: Methionylmethionine (note moved to). H-Met-Met-OH (CAS# 7349-78-2) is a dipeptide composed of two methionine residues with the molecular formula C₁₀H₂₀N₂O₃S₂ and a molecular weight of 280.41. It is also known as L-Methionyl-L-methionine and Met-Met. The compound is a dietary supplement that has been shown to have various health benefits in animals, including promoting α-s1 casein synthesis in bovine mammary gland explants. H-Met-Met-OH is orally active and is used in biochemical research on peptide transport, methionine metabolism, and protein engineering. As of current knowledge, H-Met-Met-OH is not approved as a therapeutic agent but is used as a research compound and dietary supplement. |
| Molecular Formula |
C10H20N2O3S2
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|---|---|
| Molecular Weight |
280.41
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| Exact Mass |
280.092
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| CAS # |
7349-78-2
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| PubChem CID |
6993082
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| Appearance |
Colorless to off-white solid powder
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| Density |
1.237g/cm3
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| Boiling Point |
555.1ºC at 760mmHg
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| Flash Point |
289.5ºC
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| LogP |
1.48
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
17
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| Complexity |
252
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| Defined Atom Stereocenter Count |
2
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| SMILES |
CSCC[C@H](N)C(N[C@H](C(O)=O)CCSC)=O
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| InChi Key |
ZYTPOUNUXRBYGW-YUMQZZPRSA-N
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| InChi Code |
InChI=1S/C10H20N2O3S2/c1-16-5-3-7(11)9(13)12-8(10(14)15)4-6-17-2/h7-8H,3-6,11H2,1-2H3,(H,12,13)(H,14,15)/t7-,8-/m0/s1
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| Chemical Name |
(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-4-methylsulfanylbutanoic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~356.6 mM; with ultrasonication)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (8.92 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (8.92 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (8.92 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.5662 mL | 17.8310 mL | 35.6621 mL | |
| 5 mM | 0.7132 mL | 3.5662 mL | 7.1324 mL | |
| 10 mM | 0.3566 mL | 1.7831 mL | 3.5662 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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