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| Other Sizes |
| Targets |
Bis(2-cyanoethyl) diisopropylphosphoramidite does not have a biological target as it is a chemical reagent. Its "target" in a research context is the chemical synthesis of oligonucleotides, where it serves as a phosphoramidite monomer. The compound's phosphoramidite group reacts with the 5'-hydroxyl group of nucleosides during oligonucleotide synthesis, forming phosphite triester intermediates that are subsequently oxidized to phosphates.
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| ln Vitro |
In vitro, Bis(2-cyanoethyl) diisopropylphosphoramidite is not biologically active as it is a chemical reagent. Its activity is measured by its chemical reactivity and suitability as a precursor for oligonucleotide synthesis. The compound is used in automated DNA/RNA synthesizers to incorporate phosphoramidite groups into growing oligonucleotide chains.
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| ln Vivo |
Bis(2-cyanoethyl) diisopropylphosphoramidite is not used in vivo as a therapeutic agent. It is a chemical reagent used in nucleic acid chemistry. The compound itself is not administered to animals or humans. Its in vivo effects, if any, have not been characterized as it is not a drug.
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| Enzyme Assay |
Non-cell-based assays for Bis(2-cyanoethyl) diisopropylphosphoramidite involve characterization of its chemical properties and purity. The compound's structure is confirmed by NMR and MS. Its purity is determined by HPLC or ³¹P NMR. The compound's chemical properties including molecular formula (C₁₂H₂₂N₃O₂P) and molecular weight (271.30) are characterized. Its stability and reactivity in oligonucleotide synthesis are evaluated.
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| Cell Assay |
Cellular assays for Bis(2-cyanoethyl) diisopropylphosphoramidite are not performed as the compound is a chemical reagent rather than a biologically active compound. The compound is used in chemical synthesis and is not added to cell cultures for biological evaluation.
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| Animal Protocol |
Bis(2-cyanoethyl) diisopropylphosphoramidite is not used in animal experiments as it is a chemical reagent. The compound is used in chemical synthesis and is not administered to animals. Its use is limited to nucleic acid chemistry and chemical manufacturing.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to Bis(2-cyanoethyl) diisopropylphosphoramidite as it is not a therapeutic agent. The compound is a chemical reagent used in nucleic acid chemistry. Its chemical stability, solubility, and reactivity are characterized for synthetic applications. No PK data are available as the compound is not a drug.
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| Toxicity/Toxicokinetics |
The toxicity of Bis(2-cyanoethyl) diisopropylphosphoramidite has not been comprehensively evaluated as it is not a therapeutic agent. The compound is handled as a chemical reagent with standard laboratory safety precautions. As a phosphorus-containing compound, it may be irritating to skin, eyes, and respiratory tract. The compound is for research use only and is not intended for human consumption.
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| References |
[1]. /
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| Additional Infomation |
Bis(2-cyanoethyl) diisopropylphosphoramidite (CAS# 102690-88-0) is a phosphoramidite monomer with the molecular formula C₁₂H₂₂N₃O₂P and a molecular weight of 271.30. It is also known as Bis(2-cyanoethyl)-N,N-diisopropyl Phosphoramidite. The compound is utilized in the preparation/synthesis of oligonucleotides. As of current knowledge, Bis(2-cyanoethyl) diisopropylphosphoramidite is not approved as a therapeutic agent.
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| Molecular Formula |
C12H22N3O2P
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|---|---|
| Molecular Weight |
271.30
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| Exact Mass |
271.145
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| CAS # |
102690-88-0
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| PubChem CID |
3626483
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| Appearance |
Colorless to light yellow liquid（Density：1.039 g/cm3）
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| Boiling Point |
335.3ºC at 760mmHg
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| Flash Point |
156.6ºC
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| Vapour Pressure |
0.000121mmHg at 25°C
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| LogP |
3.192
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
9
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| Heavy Atom Count |
18
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| Complexity |
286
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(C)N(C(C)C)P(OCCC#N)OCCC#N
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| InChi Key |
LDHWBEHZLFDXCU-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C12H22N3O2P/c1-11(2)15(12(3)4)18(16-9-5-7-13)17-10-6-8-14/h11-12H,5-6,9-10H2,1-4H3
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| Chemical Name |
3-[2-cyanoethoxy-[di(propan-2-yl)amino]phosphanyl]oxypropanenitrile
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~100 mg/mL (~368.6 mM; with ultrasonication)
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|---|---|
| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 2.5 mg/mL (9.21 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.21 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of 20% SBE-β-CD saline and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (9.21 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (25.0 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.6860 mL | 18.4298 mL | 36.8596 mL | |
| 5 mM | 0.7372 mL | 3.6860 mL | 7.3719 mL | |
| 10 mM | 0.3686 mL | 1.8430 mL | 3.6860 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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