| Size | Price | Stock | Qty |
|---|---|---|---|
| 100g |
|
||
| Other Sizes |
| Targets |
Peptone from casein is not a drug and does not target specific pharmacological receptors. Its role is as a complex nutrient source for microbial growth. It provides essential nitrogenous compounds, amino acids (including essential amino acids), vitamins, minerals, and peptides that support cellular metabolism and proliferation of microorganisms in laboratory culture media. It is used in fermentation processes for the production of antibiotics, enzymes, toxins, and vaccines, as well as in analytical microbiology for isolating and identifying microorganisms from pharmaceutical, environmental, food, and beverage samples.
|
|---|---|
| ln Vitro |
In vitro, Peptone from casein promotes microbial growth and proliferation. It is tested for its ability to support the growth of various indicator organisms (e.g., Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Candida albicans) in solid or liquid culture media. The compound is evaluated for total nitrogen content (12.0-14.0%) and amino nitrogen content (3.0-5.0%), ensuring consistent nutritional quality. Growth promotion tests confirm that media containing casein peptone support robust microbial growth comparable to or better than reference media.
|
| ln Vivo |
In vivo, Peptone from casein is not administered therapeutically and has no direct pharmacological activity. However, it may be used in animal studies as a component of diets or as an intraperitoneal injection to induce neutrophil recruitment or inflammatory responses for research purposes (e.g., in models of peritonitis or sterile inflammation). Its role is as an experimental tool rather than a therapeutic agent. Such applications are limited to specialized research contexts.
|
| Enzyme Assay |
Not applicable-Peptone from casein is a complex mixture of peptides and amino acids, not a single compound with a defined receptor target. Cell-free enzyme assays are not performed for this product as it is a growth medium component. Instead, its composition is characterized by analytical methods including total nitrogen determination (Kjeldahl method), amino nitrogen analysis, pH measurement, loss on drying, and sulfated ash content. Metal content (e.g., calcium ≤0.1%) may also be measured.
|
| Cell Assay |
Microbial growth promotion test protocol: Test microorganisms (e.g., E. coli ATCC 8739, S. aureus ATCC 6538, P. aeruginosa ATCC 9027, C. albicans ATCC 10231) are streaked onto agar plates containing casein peptone as the nitrogen source, or inoculated into broth media. Plates are incubated at 30-37degC for 18-48 hours, and colony morphology, growth density, and colony size are compared to reference media. For quantitative assessment, optical density at 600 nm is measured after 18-24 hours of growth in liquid culture.
|
| Animal Protocol |
Animal studies using casein peptone are not typical for drug development purposes. In specialized research (e.g., acute inflammation models), sterile casein peptone solution (e.g., 10% w/v) may be injected intraperitoneally into rodents (0.5-1 mL per mouse) to induce neutrophil recruitment. Animals are sacrificed at various time points (e.g., 4-24 hours), and peritoneal exudate is collected by lavage for differential cell counting and cytokine analysis. This model is used to study inflammatory cell migration and sterile peritonitis.
|
| ADME/Pharmacokinetics |
Not applicable-Peptone from casein is not a pharmaceutical compound, and pharmacokinetic studies are not performed. It is a complex mixture of protein hydrolysates used exclusively in vitro for microbial culture or as a laboratory reagent. The product is not intended for systemic administration to animals or humans, and its absorption, distribution, metabolism, and excretion have not been characterized and are not relevant to its intended use as a growth medium component.
|
| Toxicity/Toxicokinetics |
Peptone from casein is considered safe for handling in laboratory environments and is generally recognized as safe (GRAS) as a food ingredient or microbial growth component when used appropriately. However, inhalation of peptone powders may cause allergic respiratory reactions in sensitized individuals, and the product is classified as potentially allergenic. Safety data sheets recommend using local exhaust ventilation, wearing protective gloves and goggles, and avoiding dust generation. Ingestion or injection in research animals for non-nutritional purposes should follow institutional guidelines.
|
| Additional Infomation |
Peptone is a mixture of derived proteins or cleavage products produced by the partial hydrolysis of natural proteins by acid or enzymes. Peptone is readily soluble in water and cannot be precipitated by heating, alkali, or ammonium sulfate saturation. (Dorland, 28th edition)
See also: Paraffin (Petroleum), n-cell C5-20 (note moved to). Peptone from casein is not a drug and has no approved clinical indications. It is a standard microbiological culture medium component used for isolating, cultivating, and identifying bacteria, yeasts, and molds from pharmaceutical, environmental, food, and beverage samples. It is also used in industrial fermentation processes for producing antibiotics, toxins, enzymes, and vaccines. The product is supplied as a granulated or powdered form, typically with a total nitrogen content of 12.0-14.0%, pH 6.7-7.7 (5% solution), and solubility of 800 g/L. It may also be referred to as tryptone or peptic casein peptone. |
| Exact Mass |
5000.64
|
|---|---|
| CAS # |
91079-40-2
|
| PubChem CID |
167312541
|
| Appearance |
Light yellow to yellow solid powder
|
| Hydrogen Bond Donor Count |
8
|
| Hydrogen Bond Acceptor Count |
72
|
| Rotatable Bond Count |
46
|
| Heavy Atom Count |
370
|
| Complexity |
7410
|
| Defined Atom Stereocenter Count |
0
|
| InChi Key |
DENDBKLJAQIVGF-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C44H48O16.C40H40O16.C36H36O16.4C34H24N2O2.C34H32O16/c1-25-15-17-27(3)43(23-25)57-37(49)31(38(50)58-43)13-9-5-7-11-29-33(45)53-41(54-34(29)46)19-21-42(22-20-41)55-35(47)30(36(48)56-42)12-8-6-10-14-32-39(51)59-44(60-40(32)52)24-26(2)16-18-28(44)4;41-29-25(30(42)50-37(49-29)17-9-3-10-18-37)13-5-1-7-15-27-33(45)53-39(54-34(27)46)21-23-40(24-22-39)55-35(47)28(36(48)56-40)16-8-2-6-14-26-31(43)51-38(52-32(26)44)19-11-4-12-20-38;1-5-33(3)45-25(37)21(26(38)46-33)13-9-7-11-15-23-29(41)49-35(50-30(23)42)17-19-36(20-18-35)51-31(43)24(32(44)52-36)16-12-8-10-14-22-27(39)47-34(4,6-2)48-28(22)40;4*37-33-13-11-29(23-31(33)27-7-3-1-4-8-27)35-19-15-25(16-20-35)26-17-21-36(22-18-26)30-12-14-34(38)32(24-30)28-9-5-2-6-10-28;1-31(2)43-23(35)19(24(36)44-31)11-7-5-9-13-21-27(39)47-33(48-28(21)40)15-17-34(18-16-33)49-29(41)22(30(42)50-34)14-10-6-8-12-20-25(37)45-32(3,4)46-26(20)38/h5-14,25-28,49,51H,15-24H2,1-4H3;1-2,5-8,13-16,45,47H,3-4,9-12,17-24H2;7-16,37,39H,5-6,17-20H2,1-4H3;4*1-24H;5-14,39,41H,15-18H2,1-4H3
|
| Chemical Name |
3,12-bis[5-(2,2-dimethyl-4,6-dioxo-1,3-dioxan-5-ylidene)penta-1,3-dienyl]-4,13-dioxo-1,5,10,14-tetraoxadispiro[5.2.59.26]hexadeca-2,11-diene-2,11-diolate;3,12-bis[5-(2,4-dioxo-1,5-dioxaspiro[5.5]undecan-3-ylidene)penta-1,3-dienyl]-4,13-dioxo-1,5,10,14-tetraoxadispiro[5.2.59.26]hexadeca-2,11-diene-2,11-diolate;3-[5-[12-[5-(8,11-dimethyl-2-oxido-4-oxo-1,5-dioxaspiro[5.5]undec-2-en-3-yl)penta-2,4-dienylidene]-2,4,11,13-tetraoxo-1,5,10,14-tetraoxadispiro[5.2.59.26]hexadecan-3-ylidene]penta-1,3-dienyl]-8,11-dimethyl-4-oxo-1,5-dioxaspiro[5.5]undec-2-en-2-olate;2-ethyl-5-[5-[12-[5-(2-ethyl-2-methyl-4-oxido-6-oxo-1,3-dioxin-5-yl)penta-2,4-dienylidene]-2,4,11,13-tetraoxo-1,5,10,14-tetraoxadispiro[5.2.59.26]hexadecan-3-ylidene]penta-1,3-dienyl]-2-methyl-6-oxo-1,3-dioxin-4-olate;4-[4-[1-(4-hydroxy-3-phenylphenyl)pyridin-1-ium-4-yl]pyridin-1-ium-1-yl]-2-phenylphenol
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
