| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
| Targets |
(Rac)-CPI-203 targets BET bromodomains, particularly BRD4. It inhibits the interaction between bromodomains and acetylated lysine residues on histones, thereby modulating gene transcription.
|
|---|---|
| ln Vitro |
CPI-203 (the active enantiomer) inhibits BRD4 in vitro and in cells without affecting kinase activity. It shows cytostatic effects in 9 mantle cell lymphoma (MCL) cell lines with GI50 values ranging from 0.06 to 0.71 μM.
|
| ln Vivo |
In vivo activity is attributed to the active enantiomer CPI-203. Specific in vivo data for the racemate are not reported.
|
| Enzyme Assay |
BET bromodomain binding assays are performed using time-resolved fluorescence resonance energy transfer (TR-FRET) or AlphaScreen technologies. The compound's ability to displace a labeled probe from BRD4 is measured.
|
| Cell Assay |
Cells are treated with (Rac)-CPI-203, and cell viability is measured using MTT or CellTiter-Glo assays. The GI50 is determined from dose-response curves.
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| Animal Protocol |
In vivo animal protocols have not been established for the racemate. The active enantiomer CPI-203 would be evaluated in xenograft models.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for the racemate are not reported. The compound has a density of 1.5±0.1 g/cm³ and a boiling point of 690.5±65.0°C.
|
| Toxicity/Toxicokinetics |
Specific toxicological data for (Rac)-CPI-203 are not reported. Low cytotoxicity was observed in normal PBMCs from healthy donors.
|
| References | |
| Additional Infomation |
(Rac)-CPI-203 is a research-use-only compound. It is the racemate of the BET bromodomain inhibitor CPI-203 and is used as a reference compound in epigenetic research.
|
| Molecular Formula |
C19H18CLN5OS
|
|---|---|
| Molecular Weight |
399.90
|
| Exact Mass |
399.092
|
| CAS # |
202591-23-9
|
| Related CAS # |
CPI-203;1446144-04-2
|
| PubChem CID |
19704953
|
| Appearance |
Typically exists as solid at room temperature
|
| Density |
1.5±0.1 g/cm3
|
| Boiling Point |
690.5±65.0 °C at 760 mmHg
|
| Flash Point |
371.4±34.3 °C
|
| Vapour Pressure |
0.0±2.2 mmHg at 25°C
|
| Index of Refraction |
1.749
|
| LogP |
1.85
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
5
|
| Rotatable Bond Count |
3
|
| Heavy Atom Count |
27
|
| Complexity |
611
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
ClC1C=CC(C2=NC(CC(N)=O)C3N(C(C)=NN=3)C3SC(C)=C(C)C2=3)=CC=1
|
| InChi Key |
QECMENZMDBOLDR-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C19H18ClN5OS/c1-9-10(2)27-19-16(9)17(12-4-6-13(20)7-5-12)22-14(8-15(21)26)18-24-23-11(3)25(18)19/h4-7,14H,8H2,1-3H3,(H2,21,26)
|
| Chemical Name |
2-[7-(4-chlorophenyl)-4,5,13-trimethyl-3-thia-1,8,11,12-tetrazatricyclo[8.3.0.02,6]trideca-2(6),4,7,10,12-pentaen-9-yl]acetamide
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.5006 mL | 12.5031 mL | 25.0063 mL | |
| 5 mM | 0.5001 mL | 2.5006 mL | 5.0013 mL | |
| 10 mM | 0.2501 mL | 1.2503 mL | 2.5006 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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