| Size | Price | Stock | Qty |
|---|---|---|---|
| 500mg |
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| 1g |
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| Other Sizes |
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| Targets |
Natural withanolide with antiproliferative activity against multiple cancer cell lines, suggesting potential interactions with cellular pathways involved in cell proliferation [1].
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|---|---|
| ln Vitro |
- Physagulide J showed significant antiproliferative activity against human renal cancer cell lines (786-O, A498, ACHN) with IC₅₀ values ranging from 0.39 to 0.73 μM, and prostate cancer cell lines (CWR22R, LNCaP C4-2B) with IC₅₀ values of 0.9 μM and 0.49 μM, respectively, as assessed by MTT assay after 48 hours of treatment [1].
- In mouse RAW264.7 macrophages, Physagulide J inhibited LPS-induced nitric oxide production with an IC₅₀ of 1.36 μM, indicating anti-inflammatory activity [1]. |
| Cell Assay |
- Antiproliferative Activity Assay: Human renal cancer cells (786-O, A498, ACHN) and prostate cancer cells (CWR22R, LNCaP C4-2B) were seeded in 96-well plates and treated with Physagulide J at concentrations of 0.1-10 μM for 48 hours. Cell viability was measured using the MTT assay, and IC₅₀ values were calculated [1].
- Anti-inflammatory Activity Assay: Mouse RAW264.7 macrophages were pretreated with Physagulide J (0.1-10 μM) for 2 hours, followed by stimulation with LPS (1 μg/mL) for 24 hours. Nitric oxide production in the supernatant was quantified using the Griess assay, and the IC₅₀ value was determined [1]. |
| References | |
| Additional Infomation |
- Background and Source: Physagulide J is a withanolide compound isolated from Physalis angulata L., a traditional Chinese medicinal plant known for its anti-inflammatory and antitumor properties [1].
- Chemical Structure: It has a molecular formula of C₃₀H₄₀O₇ and a molecular weight of 512.63 [1]. - Potential Mechanism: The antiproliferative and anti-inflammatory effects of Physagulide J may be attributed to its ability to modulate cellular signaling pathways involved in cell growth and inflammation, although the exact mechanism remains to be elucidated [1]. |
| Molecular Formula |
C30H40O7
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|---|---|
| Molecular Weight |
512.63440990448
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| Exact Mass |
512.277
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| CAS # |
1496524-09-4
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| PubChem CID |
134135392
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
3.6
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
37
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| Complexity |
1140
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| Defined Atom Stereocenter Count |
11
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| SMILES |
C[C@]12C(C=CC[C@@]31O[C@@H]3C[C@@]1([H])[C@]3([C@H](C[C@]([H])([C@@H]([C@]4([H])OC(=O)C(C)=C(C)C4)C)[C@@]3(C)CC[C@]21[H])OC(=O)C)O)=O
|
| InChi Key |
IKIQWRCLUGSCEA-ITBIZAQQSA-N
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| InChi Code |
InChI=1S/C30H40O7/c1-15-12-22(36-26(33)16(15)2)17(3)20-13-25(35-18(4)31)30(34)21-14-24-29(37-24)10-7-8-23(32)28(29,6)19(21)9-11-27(20,30)5/h7-8,17,19-22,24-25,34H,9-14H2,1-6H3/t17-,19-,20+,21+,22+,24+,25-,27+,28-,29+,30+/m0/s1
|
| Chemical Name |
[(1S,2R,7S,9R,11R,12S,13S,15R,16R)-15-[(1S)-1-[(2R)-4,5-dimethyl-6-oxo-2,3-dihydropyran-2-yl]ethyl]-12-hydroxy-2,16-dimethyl-3-oxo-8-oxapentacyclo[9.7.0.02,7.07,9.012,16]octadec-4-en-13-yl] acetate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9507 mL | 9.7536 mL | 19.5072 mL | |
| 5 mM | 0.3901 mL | 1.9507 mL | 3.9014 mL | |
| 10 mM | 0.1951 mL | 0.9754 mL | 1.9507 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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