| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
DMAPP triammonium serves as a substrate for prenyltransferases and other enzymes involved in isoprenoid biosynthesis. As an isomer of IPP, it participates in the biosynthesis of all isoprenoid compounds including terpenes, steroids, and carotenoids. The compound is used in studies of metabolism-related diseases involving the mevalonate (MVA) pathway. It targets enzymes that catalyze the condensation of isoprenoid units.
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| ln Vitro |
A predecessor of an isoprenoid is DMAPP triammonium. The mevalonate pathway and the MEP pathway of isoprenoid precursor biosynthesis both produce DMAPP triammonium. Almost all living forms contain DMAPP triammonium, an isomer of isopentenyl pyrophosphate (IPP). facilitates the isomerization of IPP and DMAPP[1].
In vitro, DMAPP triammonium is used as a substrate in enzyme assays studying isoprenoid biosynthesis. The compound is utilized in biochemical experiments to investigate prenyltransferase activity, terpene synthase function, and other enzymes involved in the mevalonate and MEP pathways. It is an essential tool for studying the biosynthesis of isoprenoids and related metabolic processes. |
| ln Vivo |
In vivo, DMAPP triammonium is not used as a therapeutic agent but as a research tool for studying isoprenoid metabolism. As a precursor in the isoprenoid biosynthesis pathway, it plays a fundamental role in the production of essential biomolecules in all life forms. The compound is used in studies of metabolism-related diseases involving the mevalonate pathway.
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| Enzyme Assay |
In vitro enzyme assays for DMAPP triammonium typically involve measuring the activity of prenyltransferases or terpene synthases. The enzyme is incubated with DMAPP (as a substrate) and appropriate acceptor molecules, and product formation is monitored by HPLC, GC-MS, or radiometric methods. The compound's purity is confirmed by HPLC with purity ≥95%. Standard enzyme kinetics assays are used to determine kinetic parameters.
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| Cell Assay |
In vitro cellular assays for DMAPP triammonium are not typically performed as the compound is used as a biochemical substrate rather than a cellular modulator. However, cell-based studies of isoprenoid metabolism may involve supplementation of cell culture media with DMAPP to study its effects on isoprenoid biosynthesis. The compound is soluble in water as a triammonium salt, facilitating its use in aqueous biochemical assays.
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| Animal Protocol |
In vivo animal experiments for DMAPP triammonium are not typically performed as the compound is used as a biochemical research tool rather than a therapeutic agent. The compound is not administered to animals for pharmacological evaluation. Its applications are limited to in vitro biochemical studies of isoprenoid biosynthesis.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of DMAPP triammonium are characteristic of phosphorylated small molecules. The compound has a molecular weight of 297.18 g/mol and molecular formula C₅H₂₁N₃O₇P₂. As a highly polar phosphorylated compound, it is expected to have limited membrane permeability and oral bioavailability. It is typically used in vitro as a substrate in biochemical assays. The compound appears as a white solid.
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| Toxicity/Toxicokinetics |
The toxicological profile of DMAPP triammonium is not extensively documented. As a naturally occurring isoprenoid precursor found in virtually all life forms, it is expected to have a favorable safety profile at research concentrations. However, comprehensive toxicological evaluations have not been reported. Standard laboratory safety precautions should be followed when handling this compound.
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| References |
[1]. Wang W, et al. Bioorganometallic chemistry with IspG and IspH: structure, function, and inhibition of the [Fe(4)S(4)] proteins involved in isoprenoid biosynthesis. Angew Chem Int Ed Engl. 2014;53(17):4294-4310.
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| Additional Infomation |
DMAPP triammonium (CAS# 1186-30-7, molecular formula C₅H₂₁N₃O₇P₂, molecular weight 297.18) is an isoprenoid precursor and isomer of IPP. It is a product of both the mevalonate and MEP pathways. Used in studies of metabolism-related diseases. No clinical trials or regulatory approvals have been identified. Purity: ≥95%.
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| Molecular Formula |
C5H21N3O7P2
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|---|---|
| Molecular Weight |
297.18400
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| Exact Mass |
297.085
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| CAS # |
1186-30-7
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| Related CAS # |
DMAPP;358-72-5
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| PubChem CID |
13860073
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| Appearance |
Typically exists as solid at room temperature
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| Boiling Point |
550.8ºC at 760 mmHg
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| Flash Point |
21ºC
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| Vapour Pressure |
1.38E-13mmHg at 25°C
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| LogP |
3.622
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| Hydrogen Bond Donor Count |
3
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| Hydrogen Bond Acceptor Count |
7
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| Rotatable Bond Count |
4
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| Heavy Atom Count |
17
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| Complexity |
279
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| Defined Atom Stereocenter Count |
0
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| SMILES |
CC(=CCOP(=O)(O)OP(=O)(O)O)C.N.N.N
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3650 mL | 16.8248 mL | 33.6496 mL | |
| 5 mM | 0.6730 mL | 3.3650 mL | 6.7299 mL | |
| 10 mM | 0.3365 mL | 1.6825 mL | 3.3650 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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