| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Trifludimoxazin targets protoporphyrinogen oxidase (PPO), a critical enzyme in the chlorophyll and heme biosynthesis pathways in plants. By inhibiting PPO, the compound prevents the synthesis of chlorophyll, leading to the accumulation of protoporphyrin IX, which causes cell membrane damage and ultimately plant cell death. Susceptible plants show damage within hours and die within 3-5 days.
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| ln Vitro |
In vitro, Trifludimoxazin acts as an inhibitor of protoporphyrinogen oxidase. The compound's herbicidal activity is evaluated using standard plant-based assays measuring inhibition of photosynthesis and plant growth. As a PPO inhibitor, it prevents chlorophyll synthesis and causes cell death in susceptible plants. The compound is used as a herbicide in agricultural applications.
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| ln Vivo |
In vivo, Trifludimoxazin is applied as a herbicide in agricultural settings. Susceptible weeds show plant damage within hours of application and die within 3-5 days. The compound is effective against a wide range of weed species and is used in various crops. As a PPO inhibitor, it disrupts chlorophyll biosynthesis and causes plant death.
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| Enzyme Assay |
In vitro enzyme assays for Trifludimoxazin typically involve measuring its inhibition of protoporphyrinogen oxidase (PPO) activity. The enzyme is incubated with protoporphyrinogen IX substrate and varying concentrations of the compound, and the formation of protoporphyrin IX is monitored fluorometrically. IC₅₀ values are calculated from dose-response curves. The compound's purity is confirmed by HPLC.
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| Cell Assay |
In vitro cellular assays for Trifludimoxazin are not typically performed in mammalian cell systems, as the compound is a herbicide that targets plant PPO. In plant cell or chloroplast assays, the compound's effects on chlorophyll biosynthesis are measured. The compound's herbicidal activity is evaluated using standard plant growth inhibition assays in controlled environments.
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| Animal Protocol |
In vivo animal experiments for Trifludimoxazin are not typically performed for pharmacological evaluation, as the compound is a herbicide intended for agricultural use. However, toxicological studies in animals may be conducted for regulatory purposes to assess the compound's safety. These studies would involve oral or dermal administration to rodents, with endpoints including acute toxicity, sub-chronic toxicity, and reproductive toxicity.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Trifludimoxazin in plants involve absorption through leaves and roots, followed by translocation to target sites. The compound has a molecular weight of 412.34 g/mol and a molecular formula of C₁₆H₁₁F₃N₄O₄S. As a herbicide, it is formulated for agricultural application. The compound is a PPO inhibitor.
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| Toxicity/Toxicokinetics |
The toxicological profile of Trifludimoxazin is characteristic of PPO-inhibiting herbicides. As an agricultural chemical, its safety for humans and the environment is assessed through regulatory toxicology studies. The compound is classified as a herbicide. Standard safety precautions should be followed when handling this agricultural chemical.
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| References | |
| Additional Infomation |
Trifluoropyrazine belongs to the 1,3,5-triazine class of compounds, with the chemical formula 6-thiomethylene-1,3,5-triazine-2,4-dione. In this formula, the hydrogen atom on the nitrogen atom adjacent to the thio group is replaced by a methyl group, while the hydrogen atom on the remaining nitrogen atom is replaced by 2,2,7-trifluoro-3-oxo-4-(prop-2-yn-1-yl)-3,4-dihydro-1,4-benzoxazine-6-yl. It is a protoporphyrinogen oxidase inhibitor and is used as a herbicide. It has dual uses as a herbicide, EC 1.3.3.4 (protoporphyrinogen oxidase) inhibitor, and agrochemical. It is a benzoxazine compound, belonging to the 1,3,5-triazine class, and is also an organofluorine compound.
Trifludimoxazin (CAS# 1258836-72-4, molecular formula C₁₆H₁₁F₃N₄O₄S, molecular weight 412.34) is a Group 14 protoporphyrinogen oxidase (PPO) inhibiting herbicide. Developed by BASF. Susceptible weeds show damage within hours and die within 3-5 days. No clinical trials or regulatory approvals for therapeutic use have been identified. |
| Molecular Formula |
C16H11F3N4O4S
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|---|---|
| Molecular Weight |
412.34
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| Exact Mass |
412.045
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| CAS # |
1258836-72-4
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| PubChem CID |
49831143
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| Appearance |
Light yellow to khaki solid powder
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| LogP |
0.759
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
8
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
28
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| Complexity |
776
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| Defined Atom Stereocenter Count |
0
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| SMILES |
S=C1N(C([H])([H])[H])C(N(C(N1C([H])([H])[H])=O)C1C(=C([H])C2=C(C=1[H])N(C([H])([H])C#C[H])C(C(O2)(F)F)=O)F)=O
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| InChi Key |
AZHZOGYUMMIAOF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H11F3N4O4S/c1-4-5-22-10-7-9(8(17)6-11(10)27-16(18,19)12(22)24)23-13(25)20(2)15(28)21(3)14(23)26/h1,6-7H,5H2,2-3H3
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| Chemical Name |
1,5-dimethyl-6-sulfanylidene-3-(2,2,7-trifluoro-3-oxo-4-prop-2-ynyl-1,4-benzoxazin-6-yl)-1,3,5-triazinane-2,4-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 100 mg/mL (242.52 mM)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4252 mL | 12.1259 mL | 24.2518 mL | |
| 5 mM | 0.4850 mL | 2.4252 mL | 4.8504 mL | |
| 10 mM | 0.2425 mL | 1.2126 mL | 2.4252 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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