| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
|
||
| 100mg |
|
||
| 250mg | |||
| Other Sizes |
| Targets |
Not a drug; modifies oligonucleotide properties, enhancing solubility, reducing steric hindrance, and improving hybridization for probes and primers.
|
|---|---|
| ln Vitro |
Incorporated into Scorpion-type qPCR probes and molecular beacons to improve target accessibility; as a linker in nucleotide chain synthesis; as a spacer for biotin or other modifiers.
|
| ln Vivo |
Not applicable; used as a chemical synthesis reagent in vitro, not administered to animals for therapeutic effects.
|
| Enzyme Assay |
Standard amidite protocol: dissolve in anhydrous acetonitrile, use extended coupling time (3 min) on automated synthesizer, oxidize with iodine, cap unreacted sites.
|
| Cell Assay |
Not applicable; cell-based assays are not performed for this reagent. Instead, functional testing of synthesized oligonucleotides (e.g., qPCR efficiency) confirms successful spacer incorporation.
|
| Animal Protocol |
Not applicable; no animal studies are performed for this synthetic reagent.
|
| ADME/Pharmacokinetics |
Not applicable; not a therapeutic agent, so no PK studies are conducted.
|
| Toxicity/Toxicokinetics |
Not applicable; reagent is used in controlled laboratory settings, not for in vivo administration.
|
| References |
[1]. Longcheng Li, Moorim KANG. Multi-valent oligonucleotide agent and methods of use thereof. WO2022166849A1
[2]. Schaudy E, Lietard J, Somoza MM. Sequence Preference and Initiator Promiscuity for De Novo DNA Synthesis by Terminal Deoxynucleotidyl Transferase. ACS Synth Biol. 2021 Jul 16;10(7):1750-1760. |
| Additional Infomation |
This reagent is widely used in molecular biology for developing qPCR probes and other nucleic acid-based detection systems. It is not a drug and has no clinical status.
|
| Molecular Formula |
C42H61N2O10P
|
|---|---|
| Molecular Weight |
784.91
|
| Exact Mass |
784.406
|
| CAS # |
125607-09-2
|
| PubChem CID |
22904875
|
| Appearance |
Colorless to light yellow liquid
|
| LogP |
7.387
|
| Hydrogen Bond Donor Count |
0
|
| Hydrogen Bond Acceptor Count |
12
|
| Rotatable Bond Count |
31
|
| Heavy Atom Count |
55
|
| Complexity |
949
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
CC(N(C(C)C)P(OCCC#N)OCCOCCOCCOCCOCCOCCOC(C1=CC=C(OC)C=C1)(C1=CC=C(OC)C=C1)C1=CC=CC=C1)C
|
| InChi Key |
ZTCKUVQHKIMCLI-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C42H61N2O10P/c1-35(2)44(36(3)4)55(53-22-10-21-43)54-34-32-51-30-28-49-26-24-47-23-25-48-27-29-50-31-33-52-42(37-11-8-7-9-12-37,38-13-17-40(45-5)18-14-38)39-15-19-41(46-6)20-16-39/h7-9,11-20,35-36H,10,22-34H2,1-6H3
|
| Chemical Name |
3-[2-[2-[2-[2-[2-[2-[bis(4-methoxyphenyl)-phenylmethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy-[di(propan-2-yl)amino]phosphanyl]oxypropanenitrile
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
DMSO: 50 mg/mL (63.70 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2740 mL | 6.3702 mL | 12.7403 mL | |
| 5 mM | 0.2548 mL | 1.2740 mL | 2.5481 mL | |
| 10 mM | 0.1274 mL | 0.6370 mL | 1.2740 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
