| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Sperm motility agonist-2 targets soluble adenylyl cyclase (sAC), a key enzyme in the cAMP signaling pathway. By acting as a potent sAC agonist, it increases intracellular cAMP levels, which in turn enhances sperm motility and hyperactivation. The compound may also affect mitochondrial function. Its activity on sAC makes it a valuable tool for studying sperm function and fertility.
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| ln Vitro |
In vitro, Sperm motility agonist-2 acts as a potent sAC agonist, increasing cAMP levels and enhancing sperm motility and hyperactivation. The compound is used in sperm function assays to evaluate its effects on sperm motility parameters. It may also affect mitochondrial function. However, detailed in vitro activity data including EC₅₀ values have not been extensively published.
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| ln Vivo |
In vivo, Sperm motility agonist-2 may be used in studies of infertility and fertility enhancement. As a sperm motility agonist, it has potential applications in enhancing fertility in animal models or in assisted reproductive technologies. However, specific in vivo study results have not been extensively detailed in the available literature.
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| Enzyme Assay |
In vitro assays for Sperm motility agonist-2 typically involve evaluating its effects on sperm motility. Sperm samples are treated with varying concentrations of the compound, and sperm motility parameters (progressive motility, velocity, hyperactivation) are measured using computer-assisted sperm analysis (CASA). cAMP levels in sperm can be measured by ELISA or other immunoassays. The compound's purity is confirmed by HPLC (≥99.67%).
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| Cell Assay |
In vitro cellular assays for Sperm motility agonist-2 typically utilize sperm cells or cell lines expressing sAC. Sperm cells are treated with the compound, and motility parameters are assessed by CASA. cAMP levels are measured by ELISA. Mitochondrial function can be assessed using mitochondrial membrane potential dyes or ATP measurement. The compound is soluble in DMSO (200 mg/mL).
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| Animal Protocol |
In vivo animal experiments for Sperm motility agonist-2 have not been extensively reported. Based on its mechanism as a sAC agonist and sperm motility enhancer, potential in vivo studies could involve rodent models to evaluate fertility enhancement. However, specific study protocols and results have not been published for this compound.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Sperm motility agonist-2 have not been extensively characterized. The compound has a molecular weight of 334.82 g/mol and a molecular formula of C₁₆H₁₅ClN₂O₂S. As a small molecule with heterocyclic aromatic moieties, it is expected to have reasonable membrane permeability. Solubility: DMSO 200 mg/mL. Storage: powder at -20°C for 3 years; in solvent at -80°C for 1 year.
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| Toxicity/Toxicokinetics |
The toxicological profile of Sperm motility agonist-2 has not been extensively documented. As a research compound targeting sAC, comprehensive toxicological evaluations including acute toxicity, genotoxicity, and repeated-dose studies would be required for therapeutic development. Standard laboratory safety precautions should be followed when handling this compound.
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| References |
[1]. Mannowetz, et al. Compositions and methods of use for enhancing fertility. World Intellectual Property Organization, WO2021046446 A1. 2021-03-11.
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| Additional Infomation |
Sperm motility agonist-2 (CAS# 926079-67-6, molecular formula C₁₆H₁₅ClN₂O₂S, molecular weight 334.82) is a potent soluble adenylyl cyclase (sAC) agonist. It enhances sperm motility and hyperactivation by increasing cAMP levels. Also known as compound 797. Used in infertility and fertility enhancement research. No clinical trials or regulatory approvals have been identified. Purity ≥99.67%.
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| Molecular Formula |
C16H15CLN2O2S
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|---|---|
| Molecular Weight |
334.820501565933
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| Exact Mass |
334.054
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| CAS # |
926079-67-6
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| PubChem CID |
16504541
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| Appearance |
White to off-white solid powder
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| LogP |
4.6
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
6
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| Heavy Atom Count |
22
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| Complexity |
350
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| Defined Atom Stereocenter Count |
0
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| SMILES |
ClC1C=CC=CC=1OCCSC1=NC2C=CC(=CC=2N1)OC
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| InChi Key |
FIVXXCJBDPVCJR-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C16H15ClN2O2S/c1-20-11-6-7-13-14(10-11)19-16(18-13)22-9-8-21-15-5-3-2-4-12(15)17/h2-7,10H,8-9H2,1H3,(H,18,19)
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| Chemical Name |
2-[2-(2-chlorophenoxy)ethylsulfanyl]-6-methoxy-1H-benzimidazole
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: 250 mg/mL (746.67 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9867 mL | 14.9334 mL | 29.8668 mL | |
| 5 mM | 0.5973 mL | 2.9867 mL | 5.9734 mL | |
| 10 mM | 0.2987 mL | 1.4933 mL | 2.9867 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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