| Size | Price | Stock | Qty |
|---|---|---|---|
| 25g |
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| Other Sizes |
Purity: ≥98%
| Targets |
Beef extract does not have specific pharmacological targets, as it is a nutrient supplement for cell and microbial culture rather than a therapeutic agent. It provides essential nutrients that support the growth and metabolism of microorganisms and cells in culture. The complex mixture of components in beef extract serves as a source of nitrogen, carbon, vitamins, and minerals for cultured organisms.
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|---|---|
| ln Vitro |
Beef extract is not evaluated for traditional in vitro biological activity as it is a culture medium component rather than a pharmacologically active compound. Its utility in research is based on its nutritional properties for supporting microbial and cell growth. It provides essential nutrients for the cultivation of various bacteria and cells. No specific biological activity data have been reported.
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| ln Vivo |
Beef extract is not used for in vivo pharmacological evaluation. It is a culture medium component used in laboratory research for growing microorganisms and cells. The compound is not administered to animals for therapeutic purposes. Its applications are limited to in vitro cell and microbial culture.
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| Enzyme Assay |
Beef extract is not evaluated in traditional enzyme/receptor binding assays. It is used as a component of biological culture media. The compound's composition can be characterized by measuring total nitrogen (≥12.0%) and amino nitrogen (≥3%) content. Its utility is assessed by its ability to support the growth of microorganisms in culture.
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| Cell Assay |
Beef extract is not evaluated in traditional cellular assays as a therapeutic compound. It is used as a nutrient supplement in cell culture media to support the growth of various cell types. Cells are cultured in media supplemented with beef extract, and cell growth and viability are assessed. The compound provides essential nutrients for cell proliferation.
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| Animal Protocol |
Beef extract is not used in in vivo animal experiments as a pharmacological agent. It is a culture medium component used in laboratory research for growing microorganisms and cells. The compound is not administered to animals for therapeutic or pharmacological assessment.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to beef extract, as it is a culture medium component rather than a therapeutic agent. Beef extract is a complex mixture with variable composition. It contains soluble nitrogenous compounds (approximately 50%). It is a viscous liquid at room temperature, with viscosity decreasing as temperature increases.
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| Toxicity/Toxicokinetics |
The toxicological profile of beef extract is consistent with its use as a culture medium component. Hazard classifications include Eye Irrit. 2 (H319). Standard laboratory safety precautions should be followed when handling this compound. It is not intended for human consumption.
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| References | |
| Additional Infomation |
N-Cyclohexyl-2-aminoethanesulfonic acid is an alkylsulfonic acid, a derivative of cyclohexylamine, in which one amino hydrogen atom is replaced by a 2-sulfoethyl group. It is used as a buffer (pH range 8.6–10.0) in enzymatic studies of pH-dependent processes. It is a Goodyear buffer. It is an alkylsulfonic acid and a secondary fatty amine.
See also: Beef extract (note moved to). Beef extract (CAS# 68990-09-0) is a nutrient-rich component commonly used in the preparation of biological culture media. It provides peptides, amino acids, nucleotides, vitamins, and minerals. Used for culturing E. coli, B. subtilis, and other bacteria. No clinical trials or regulatory approvals have been identified. |
| Molecular Formula |
C14H18CLNO4
|
|---|---|
| Molecular Weight |
299.750023365021
|
| Exact Mass |
207.092
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| CAS # |
68990-09-0
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| Related CAS # |
134737-05-6;3076-05-9 (mono-hydrochloride salt)
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| PubChem CID |
66898
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| Appearance |
Light yellow to yellow solid powder
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| LogP |
-1.2
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| Hydrogen Bond Donor Count |
2
|
| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
4
|
| Heavy Atom Count |
13
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| Complexity |
226
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| Defined Atom Stereocenter Count |
0
|
| SMILES |
ClC1C=CC(=CC=1)C[C@@H](C(=O)O)NC(=O)OC(C)(C)C
|
| InChi Key |
MKWKNSIESPFAQN-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H17NO3S/c10-13(11,12)7-6-9-8-4-2-1-3-5-8/h8-9H,1-7H2,(H,10,11,12)
|
| Chemical Name |
2-(cyclohexylamino)ethanesulfonic acid
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O: 20 mg/mL
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3361 mL | 16.6806 mL | 33.3611 mL | |
| 5 mM | 0.6672 mL | 3.3361 mL | 6.6722 mL | |
| 10 mM | 0.3336 mL | 1.6681 mL | 3.3361 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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