| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
The specific molecular targets of 5-hydroxy-3,7-dimethoxyflavone are not well characterized in the available literature. As a flavonoid, it may interact with various cellular pathways involved in oxidative stress, inflammation, and cell signaling. Flavonoids are known to modulate enzyme activities, receptor functions, and gene expression through multiple mechanisms. However, studies indicate that this particular compound does not exhibit significant antimicrobial activity against malaria parasites, fungi, or bacteria, suggesting limited activity against these specific targets.
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| ln Vitro |
In vitro, 5-hydroxy-3,7-dimethoxyflavone (compound 1) has been evaluated for its biological activities but shows no significant toxicity to malaria parasites, fungi, and bacteria. This lack of antimicrobial activity suggests that the compound may have limited efficacy against these pathogens in vitro. As a flavonoid, it may exhibit other activities such as antioxidant or anti-inflammatory effects, but specific IC50 values or detailed mechanistic data are not extensively reported in the available literature. The compound is primarily used as a natural product standard.
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| ln Vivo |
Specific in vivo data for 5-hydroxy-3,7-dimethoxyflavone are not reported in the available literature. As a compound isolated from Kaempferia parviflora, which has traditional medicinal uses, it may contribute to the overall pharmacological effects of the plant extract. However, studies on the isolated compound in animal models have not been detailed. Its lack of toxicity to certain pathogens suggests it may be safe for further investigation, but comprehensive in vivo efficacy studies are needed to determine its therapeutic potential.
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| Enzyme Assay |
The in vitro biological activity of 5-hydroxy-3,7-dimethoxyflavone is typically assessed using standard antimicrobial assays. For antibacterial testing, bacterial strains are cultured in appropriate broth media and treated with the compound at various concentrations. The minimum inhibitory concentration (MIC) is determined as the lowest concentration that inhibits visible growth. For antifungal testing, fungal strains are cultured and treated similarly. For antimalarial testing, Plasmodium parasites are cultured in erythrocytes and treated with the compound, with parasite growth measured by microscopy or fluorescence.
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| Cell Assay |
For cellular studies, various cell lines such as cancer cells, immune cells, or pathogen-infected cells are cultured in appropriate media. 5-Hydroxy-3,7-dimethoxyflavone is dissolved in DMSO and diluted in culture media at various concentrations (typically 0.1-100 μM). Cells are treated for 24-72 hours, and cell viability is assessed using MTT or similar assays. For antimicrobial studies, pathogen growth is monitored by optical density or colony counting. The compound is typically stored as a powder at -20°C and protected from light.
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| Animal Protocol |
In vivo studies for 5-hydroxy-3,7-dimethoxyflavone would be conducted in appropriate animal models depending on the research focus. For antimicrobial studies, infection models using relevant pathogens would be used. For cancer research, xenograft models could be employed. However, specific published protocols for this compound are not available. The compound would typically be administered via oral gavage or intraperitoneal injection, and endpoints would depend on the disease model being studied.
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| ADME/Pharmacokinetics |
Specific pharmacokinetic data for 5-hydroxy-3,7-dimethoxyflavone are not reported in the available literature. As a flavonoid with methoxy substitutions, the compound is expected to have moderate lipophilicity and potential for oral absorption. However, flavonoids generally have variable bioavailability due to extensive metabolism in the gastrointestinal tract and liver. Pharmacokinetic studies would be required to determine parameters such as half-life, Cmax, oral bioavailability, and tissue distribution in animal models.
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| Toxicity/Toxicokinetics |
5-Hydroxy-3,7-dimethoxyflavone has been reported to have no significant toxicity to malaria parasites, fungi, and bacteria, suggesting a favorable safety profile against these organisms. As a natural product from Kaempferia parviflora, which has traditional medicinal uses, the compound is generally considered to have a moderate safety profile. However, comprehensive toxicology studies including acute, subchronic, and genotoxicity assessments have not been reported. The compound should be handled with appropriate safety precautions in laboratory settings.
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| References | |
| Additional Infomation |
4H-1-benzopyran-4-one, 5-hydroxy-3,7-dimethoxy-2-phenyl- has been reported to exist in Boesenbergia rotunda, Heliotropium marifolium, and other organisms for which data are available.
5-Hydroxy-3,7-dimethoxyflavone is a natural flavonoid isolated from Kaempferia parviflora. It exhibits a range of biological activities and is noted for its potential role in research exploring the modulation of cellular pathways. However, it has no significant toxicity to malaria parasites, fungi, and bacteria. No clinical trials or regulatory approvals exist. For research use only. |
| Molecular Formula |
C17H14O5
|
|---|---|
| Molecular Weight |
298.29
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| Exact Mass |
298.084
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| CAS # |
70786-48-0
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| PubChem CID |
5748697
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| Appearance |
Off-white to light yellow solid powder
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| LogP |
3.182
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
22
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| Complexity |
452
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
OYCOUDKDRFJOCP-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C17H14O5/c1-20-11-8-12(18)14-13(9-11)22-16(17(21-2)15(14)19)10-6-4-3-5-7-10/h3-9,18H,1-2H3
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| Chemical Name |
5-hydroxy-3,7-dimethoxy-2-phenylchromen-4-one
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.3524 mL | 16.7622 mL | 33.5244 mL | |
| 5 mM | 0.6705 mL | 3.3524 mL | 6.7049 mL | |
| 10 mM | 0.3352 mL | 1.6762 mL | 3.3524 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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