| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
The molecular targets of 1,2-Bis(maleimido)ethane are sulfhydryl groups (thiols) on proteins and peptides. The maleimide groups react specifically with thiols at pH 6.5-7.5 to form stable thioether bonds. The compound does not have a specific biological target but is used to crosslink proteins and peptides. It is a chemical tool for studying protein-protein interactions and for preparing protein conjugates.
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| ln Vitro |
In vitro activity of 1,2-Bis(maleimido)ethane is demonstrated by its ability to crosslink proteins and peptides. The compound reacts with sulfhydryl groups to form stable covalent bonds, enabling the conjugation of proteins or peptides. Its crosslinking efficiency can be assessed by SDS-PAGE or mass spectrometry, where the formation of higher molecular weight species indicates successful crosslinking. The compound's reactivity with thiols makes it a valuable tool for protein chemistry.
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| ln Vivo |
In vivo studies of 1,2-Bis(maleimido)ethane are not typically conducted as it is a chemical crosslinking reagent used in vitro. The compound is not intended for therapeutic or pharmacological use. No in vivo studies have been reported. Its applications are limited to biochemical and polymer chemistry research.
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| Enzyme Assay |
Typical in vitro assays for 1,2-Bis(maleimido)ethane involve crosslinking reactions with proteins or peptides containing free thiol groups. The compound is added to the protein solution at molar ratios ranging from 1:1 to 10:1 (crosslinker:protein) in buffer at pH 6.5-7.5. The reaction is incubated at room temperature for 30-120 minutes. Crosslinking efficiency is assessed by SDS-PAGE, where the appearance of higher molecular weight bands indicates crosslinked products. For peptide conjugation, the reaction can be monitored by HPLC or mass spectrometry.
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| Cell Assay |
Cell-based assays for 1,2-Bis(maleimido)ethane are not typically performed as the compound is used in cell-free systems for protein crosslinking. For studying protein-protein interactions, the crosslinked proteins are typically analyzed by biochemical methods. The compound is not used in cell-based assays due to its reactivity with cellular thiols and potential cytotoxicity.
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| Animal Protocol |
In vivo animal experiments for 1,2-Bis(maleimido)ethane are not conducted as the compound is a chemical crosslinking reagent. It is not used in animal studies due to its reactivity with thiols, which would make it unsuitable for in vivo applications due to non-specific crosslinking of proteins and potential toxicity.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 1,2-Bis(maleimido)ethane are not relevant as the compound is a chemical reagent, not a therapeutic agent. It is not intended for systemic administration. The compound is used in vitro and is not absorbed or distributed in the body. No PK studies have been reported.
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| Toxicity/Toxicokinetics |
Toxicological data for 1,2-Bis(maleimido)ethane are limited. As a chemical crosslinking reagent, it is used in small quantities in laboratory settings. The compound's maleimide groups are reactive and may cause skin and eye irritation. Standard laboratory safety precautions should be followed when handling the compound. No significant systemic toxicity has been reported.
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| References | |
| Additional Infomation |
1,2-Bis(maleimido)ethane (BMOE) is a homobifunctional protein crosslinking agent that reacts with sulfhydryl groups. It forms stable covalent bonds between proteins or peptides and is used in biochemical applications for protein conjugation and crosslinking. The compound is also used in polymer chemistry as a crosslinking agent. It is a research chemical and is not approved for any clinical indication.
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| Molecular Formula |
C10H8N2O4
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|---|---|
| Molecular Weight |
220.18
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| Exact Mass |
220.048
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| CAS # |
5132-30-9
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| PubChem CID |
237509
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| Appearance |
Off-white to light yellow solid powder
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| Density |
1.5±0.1 g/cm3
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| Boiling Point |
428.3±28.0 °C at 760 mmHg
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| Melting Point |
193-194°C
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| Flash Point |
215.7±16.4 °C
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| Vapour Pressure |
0.0±1.0 mmHg at 25°C
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| Index of Refraction |
1.619
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| LogP |
-0.14
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
4
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
16
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| Complexity |
372
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
PUKLCKVOVCZYKF-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H8N2O4/c13-7-1-2-8(14)11(7)5-6-12-9(15)3-4-10(12)16/h1-4H,5-6H2
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| Chemical Name |
1-[2-(2,5-dioxopyrrol-1-yl)ethyl]pyrrole-2,5-dione
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5417 mL | 22.7087 mL | 45.4174 mL | |
| 5 mM | 0.9083 mL | 4.5417 mL | 9.0835 mL | |
| 10 mM | 0.4542 mL | 2.2709 mL | 4.5417 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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