| Size | Price | |
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| Other Sizes |
| Targets |
The primary molecular target of 1,2,4-Triazole sodium is cytochrome P450 enzymes. By inhibiting these enzymes, it can affect the metabolism of various substrates, including drugs and xenobiotics. It has been shown to have a biological effect on oxygen nucleophiles and can be used as an anti-inflammatory agent. It may also interact with other targets involved in antifungal and herbicidal activity.
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| ln Vitro |
In vitro, 1,2,4-Triazole sodium serves as a potent inhibitor of cytochrome P450 enzymes. It plays a critical role in biochemical research, particularly in studies involving antifungal and herbicidal activity. It has been shown to have a biological effect on oxygen nucleophiles and can be used as an anti-inflammatory agent. However, specific potency data such as IC₅₀ values are not detailed in the available sources.
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| ln Vivo |
Specific in vivo activity data for 1,2,4-Triazole sodium are not available in the consulted sources. As a cytochrome P450 inhibitor, it could affect the metabolism of drugs and other compounds in vivo. Its antifungal and herbicidal activities suggest potential agricultural applications. However, no specific in vivo studies in animal models have been reported.
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| Enzyme Assay |
No specific protocols for enzyme/receptor binding assays are available for 1,2,4-Triazole sodium. As a cytochrome P450 inhibitor, standard assays involve measuring the metabolism of P450 substrates (e.g., CYP3A4, CYP2D6 substrates) in the presence of various concentrations of the compound. IC₅₀ values are calculated from dose-response curves. Antifungal activity is assessed by broth microdilution assays to determine MIC values.
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| Cell Assay |
Cellular assays for 1,2,4-Triazole sodium typically involve treating hepatocytes or other cells expressing cytochrome P450 enzymes with the compound at concentrations ranging from 1-100 µM for 24-48 hours. P450 activity is measured by incubating cells with P450 substrates and measuring metabolite production. Cell viability is assessed by MTT assay. These cell-based systems allow for detailed analysis of the compound's P450 inhibitory activity.
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| Animal Protocol |
In vivo animal experiments for 1,2,4-Triazole sodium are not detailed in the available literature. As a cytochrome P450 inhibitor, it could be used in drug interaction studies in animals. However, no specific studies have been reported. Its use is primarily in biochemical research and as a reagent.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 1,2,4-Triazole sodium are limited. As a small, polar molecule with a molecular weight of 91.05 g/mol, it would be expected to have good aqueous solubility and rapid renal excretion. However, detailed ADME parameters are not available in the consulted sources. The compound is intended for research use only.
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| Toxicity/Toxicokinetics |
Toxicological data for 1,2,4-Triazole sodium are limited. As a biochemical reagent, it is used in small quantities in laboratory settings. Standard laboratory safety precautions should be followed when handling the compound. No significant toxicity has been reported.
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| References |
[1]. Vishnu Ji Ram, et al. 1,2,4-triazole. The Chemistry of Heterocycles, 2019.
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| Additional Infomation |
1,2,4-Triazole sodium is a biochemical reagent that serves as a potent inhibitor of cytochrome P450 enzymes. It is used in research involving antifungal and herbicidal activity and can be used as an anti-inflammatory agent. It is a research compound for studying drug metabolism, antifungal agents, and herbicides. It is not approved for any clinical indication.
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| Molecular Formula |
C2H3N3NA
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|---|---|
| Molecular Weight |
92.06
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| Exact Mass |
91.014
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| CAS # |
41253-21-8
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| Related CAS # |
1,2,4-Triazole;288-88-0;1,2,4-Triazole-13C2,15N3;1261170-82-4;1,2,4-Triazole-d3;43088-92-2
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| PubChem CID |
4649906
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| Appearance |
White to off-white solid powder
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| Density |
1.255
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| Boiling Point |
260ºC at 760 mmHg
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| Melting Point |
295 °C (dec.)(lit.)
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| Flash Point |
139.1ºC
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
6
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| Complexity |
32
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[Na+].[N-]1C([H])=NC([H])=N1
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| InChi Key |
NVMNEWNGLGACBB-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C2H2N3.Na/c1-3-2-5-4-1;/h1-2H;/q-1;+1
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| Chemical Name |
sodium;2,4-diaza-1-azanidacyclopenta-2,4-diene
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 10.8625 mL | 54.3124 mL | 108.6248 mL | |
| 5 mM | 2.1725 mL | 10.8625 mL | 21.7250 mL | |
| 10 mM | 1.0862 mL | 5.4312 mL | 10.8625 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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