| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
5′-Guanylyl methylenediphosphonate targets GTP-binding proteins (GTPases) by acting as a non-hydrolyzable GTP analog. It binds to the GTP-binding sites of proteins such as G-proteins, small GTPases (e.g., Ras, Rho), and other nucleotide-binding proteins, stabilizing them in their active conformation without being hydrolyzed. This makes it a valuable tool for studying GTPase function and signaling pathways.
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| ln Vitro |
In vitro, 5′-Guanylyl methylenediphosphonate is used as a non-hydrolyzable GTP analog to study GTP-binding proteins and GTPase activity. It binds to GTP-binding sites, allowing researchers to investigate nucleotide-dependent processes without the complication of GTP hydrolysis. It is used in assays measuring GTP binding, GTPase activity, and downstream signaling events. However, specific potency data are not applicable as this is a research tool rather than a bioactive compound.
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| ln Vivo |
Specific in vivo activity data for 5′-Guanylyl methylenediphosphonate are not available as it is a biochemical research tool, not a pharmacologically active agent. It is not intended for therapeutic in vivo studies. Its applications are limited to in vitro biochemical research for studying GTP-binding proteins and nucleotide-dependent processes.
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| Enzyme Assay |
Typical in vitro assays for 5′-Guanylyl methylenediphosphonate involve GTP-binding assays where the compound is used as a non-hydrolyzable GTP analog. It is incubated with GTP-binding proteins, and binding is measured by filter-binding assays or surface plasmon resonance. GTPase activity assays use the compound as a control to demonstrate GTPase-dependent processes. These assays are standard for studying G-proteins and small GTPases.
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| Cell Assay |
Cell-based assays for 5′-Guanylyl methylenediphosphonate are not typically performed as the compound is not cell-permeable due to its negative charges. It is primarily used in cell-free systems or in permeabilized cells. For studying GTPase function, cell lysates or purified proteins are used with the compound to investigate nucleotide-dependent processes and signaling pathways.
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| Animal Protocol |
No specific in vivo animal experiment protocols are available for 5′-Guanylyl methylenediphosphonate. As a non-hydrolyzable GTP analog, it is not used in animal studies for therapeutic or pharmacological purposes. Its applications are limited to in vitro biochemical research.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for 5′-Guanylyl methylenediphosphonate are not applicable as it is a biochemical research tool, not a therapeutic agent. It is not intended for systemic administration. The compound is not cell-permeable and is used in cell-free systems. No ADME studies have been reported.
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| Toxicity/Toxicokinetics |
Toxicological data for 5′-Guanylyl methylenediphosphonate are limited. As a nucleotide analog used in biochemical research, it is used in small quantities in laboratory settings. Standard laboratory safety precautions should be followed when handling the compound. No significant toxicity has been reported.
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| References | |
| Additional Infomation |
Guanosine 5'-[β,γ-methylene]triphosphate trisodium is an organosodium salt, specifically the trisodium salt of guanosine 5'-(β,γ-methylene)triphosphate. It contains guanosine 5'-[β,γ-methylene]triphosphate (4-).
5′-Guanylyl methylenediphosphonate sodium (β,γ-Methylene-GTP sodium) is a non-hydrolyzable analog of guanosine triphosphate (GTP) used as a biochemical tool to study GTP-binding proteins and GTPase activity. It binds to GTP-binding sites, stabilizing proteins in their active conformation. It is a research reagent and is not approved for any clinical indication. |
| Molecular Formula |
C11H15N5NA3O13P3
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|---|---|
| Molecular Weight |
587.15
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| Exact Mass |
544.001
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| CAS # |
10470-57-2
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| PubChem CID |
136234287
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| Appearance |
White to off-white solid powder
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| Hydrogen Bond Donor Count |
5
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| Hydrogen Bond Acceptor Count |
15
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| Rotatable Bond Count |
7
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| Heavy Atom Count |
35
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| Complexity |
899
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| Defined Atom Stereocenter Count |
4
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| SMILES |
[NaH].OP(CP(OP(OCC1OC(N2C=NC3C(N=C(NC2=3)N)=O)C(O)C1O)(O)=O)(O)=O)(O)=O
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| InChi Key |
BOCIJVWLZVQKJK-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C11H18N5O13P3.Na/c12-11-14-8-5(9(19)15-11)13-2-16(8)10-7(18)6(17)4(28-10)1-27-32(25,26)29-31(23,24)3-30(20,21)22;/h2,4,6-7,10,17-18H,1,3H2,(H,23,24)(H,25,26)(H2,20,21,22)(H3,12,14,15,19);
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7031 mL | 8.5157 mL | 17.0314 mL | |
| 5 mM | 0.3406 mL | 1.7031 mL | 3.4063 mL | |
| 10 mM | 0.1703 mL | 0.8516 mL | 1.7031 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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