| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| Other Sizes |
| Targets |
Acetyl-CoA synthetase targets acetate as its substrate, converting it to acetyl-CoA. The enzyme catalyzes the reaction: ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA. It also acts on propanoate and propenoate as substrates. The enzyme's activity is essential for cellular metabolism and energy production.
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| ln Vitro |
Acetyl-CoA synthetase is an enzyme, not a drug, and is used as a biochemical reagent in research. Its activity is measured in vitro by monitoring the production of acetyl-CoA from acetate and ATP. The enzyme is available as a lyophilized powder with a specific activity of ≥3 units/mg protein.
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| ln Vivo |
Acetyl-CoA synthetase is an enzyme that functions in vivo as part of cellular metabolism. It catalyzes the production of acetyl-CoA, a key metabolite in energy production and biosynthesis. The enzyme is involved in histone acetylation in the nucleus.
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| Enzyme Assay |
Typical in vitro assays for Acetyl-CoA synthetase involve measuring the enzyme's activity using radioactive or spectrophotometric methods. The enzyme is incubated with ATP, acetate, and CoA, and the production of acetyl-CoA is measured. Alternatively, the formation of AMP or the consumption of ATP can be monitored. The enzyme's activity can be inhibited by various compounds for research purposes.
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| Cell Assay |
Acetyl-CoA synthetase is an enzyme and is not used in cell-based assays as a test compound. It is used as a reagent in cell lysates or purified form to study metabolic pathways. Cells can be treated with inhibitors of ACS to study the effects on cellular metabolism.
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| Animal Protocol |
Acetyl-CoA synthetase is an enzyme and is not administered to animals as a therapeutic agent. It is used as a biochemical tool in research. Animal models may be used to study the effects of ACS inhibition on metabolism.
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| ADME/Pharmacokinetics |
Pharmacokinetic data are not applicable for Acetyl-CoA synthetase as it is an enzyme, not a therapeutic agent. The enzyme is not systemically administered.
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| Toxicity/Toxicokinetics |
Toxicological data are not applicable for Acetyl-CoA synthetase as it is an enzyme used in research. Standard laboratory safety precautions should be followed when handling the enzyme preparation.
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| Additional Infomation |
Acetyl-CoA synthetase (ACS, EC 6.2.1.1) is an enzyme that catalyzes the production of acetyl-CoA from acetate, ATP, and CoA. It is involved in glycolysis, gluconeogenesis, pyruvate metabolism, and histone acetylation. The enzyme is available as a lyophilized powder from baker's yeast for research use. It is not a drug and has no clinical applications.
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| Molecular Weight |
0
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|---|---|
| Exact Mass |
313.054
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| CAS # |
9012-31-1
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.6±0.1 g/cm3
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| Boiling Point |
583.2±50.0 °C at 760 mmHg
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| Flash Point |
306.5±30.1 °C
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| Vapour Pressure |
0.0±1.7 mmHg at 25°C
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| Index of Refraction |
1.636
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| LogP |
0.43
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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