| Size | Price | Stock | Qty |
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| 1mg |
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| 5mg |
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| Other Sizes |
| Targets |
Euphorbia factor L7b targets the NF-κB signaling pathway and induces apoptosis in cancer cells. Its anti-inflammatory effects are attributed to modulation of key signaling pathways. As a natural diterpenoid from Euphorbia pekinensis, a plant used in traditional herbal medicine, its cytotoxic effects suggest additional targets in cancer cells.
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| ln Vitro |
In vitro studies of Euphorbia factor L7b have demonstrated anti-inflammatory and cytotoxic effects. The compound modulates signaling pathways such as NF-κB and induces apoptosis. However, specific potency data such as IC₅₀ values against particular cell lines are not detailed in the available literature.
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| ln Vivo |
Specific in vivo activity data for Euphorbia factor L7b are not available in the consulted sources. As a natural product with anti-inflammatory and cytotoxic effects, it may have potential in vivo efficacy in models of cancer and inflammation. However, no systematic in vivo studies have been reported.
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| Enzyme Assay |
Typical in vitro assays for Euphorbia factor L7b include anti-inflammatory assays measuring NO production in LPS-stimulated macrophages. Cytotoxicity assays are performed using cancer cell lines treated with the compound for 48-72 hours, and cell viability is assessed by MTT or similar assays. NF-κB signaling is assessed by reporter gene assays or Western blot.
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| Cell Assay |
Cellular assays for Euphorbia factor L7b typically involve treating cancer cell lines or macrophages with the compound at concentrations ranging from 1-100 µM. Cell viability is assessed by MTT assay. Apoptosis is evaluated by Annexin V/PI staining and caspase activity measurements. NF-κB activation is assessed by Western blot or reporter gene assays.
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| Animal Protocol |
In vivo animal experiments for Euphorbia factor L7b have not been reported in the available literature. For potential anticancer or anti-inflammatory studies, typical animal models such as xenograft or inflammation models could be employed, but no specific protocols have been established.
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| ADME/Pharmacokinetics |
Pharmacokinetic data for Euphorbia factor L7b are not available in the consulted sources. As a diterpenoid with a molecular weight of 580.67 g/mol, it would be expected to have moderate lipophilicity. Detailed ADME parameters have not been characterized.
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| Toxicity/Toxicokinetics |
No toxicological data are available for Euphorbia factor L7b in the consulted sources. As a natural product from Euphorbia pekinensis, a plant used in traditional medicine, it is generally considered to have low toxicity, but comprehensive toxicological studies have not been reported.
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| References | |
| Additional Infomation |
It has been reported that plants in the Euphorbia genus contain the Euphorbia factor L7b, and relevant data are available for reference.
Euphorbia factor L7b is a natural diterpenoid (isolathyrolditerpene) from Euphorbia pekinensis Rupr. It exhibits anti-inflammatory and cytotoxic effects through NF-κB modulation and apoptosis induction. It is a research compound for studying cancer and inflammation. It is not approved for any clinical indication. |
| Molecular Formula |
C33H40O9
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|---|---|
| Molecular Weight |
580.67
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| Exact Mass |
580.267
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| CAS # |
93550-95-9
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| PubChem CID |
156599174
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| Appearance |
White to off-white solid
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| Source |
Originated from plants: other families
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| LogP |
4.782
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
9
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| Rotatable Bond Count |
10
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| Heavy Atom Count |
42
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| Complexity |
1170
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| Defined Atom Stereocenter Count |
7
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| SMILES |
CC1CC2(C(C1OC(=O)C3=CC=CC=C3)C(C(=CCC4C(C4(C)C)C=C(C2=O)C)COC(=O)C)OC(=O)C)OC(=O)C
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| InChi Key |
ARUXHDLPKVRONO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C33H40O9/c1-18-15-26-25(32(26,6)7)14-13-24(17-39-20(3)34)29(40-21(4)35)27-28(41-31(38)23-11-9-8-10-12-23)19(2)16-33(27,30(18)37)42-22(5)36/h8-13,15,19,25-29H,14,16-17H2,1-7H3
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| Chemical Name |
[1,11-diacetyloxy-10-(acetyloxymethyl)-3,6,6,14-tetramethyl-2-oxo-13-tricyclo[10.3.0.05,7]pentadeca-3,9-dienyl] benzoate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO: ~50 mg/mL (~86.1 mM; with ultrasonication)
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| Solubility (In Vivo) |
Solubility in Formulation 1: ≥ 1.25 mg/mL (2.15 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), Clear solution.
For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 400 μL of PEG300 and mix well; then add 50 μL of Tween-80 and mix well; finally add 450 μL of physiological saline and adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.25 mg/mL (2.15 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), Clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of DMSO stock solution (12.5 mg/mL) to 900 μL of corn oil and mix well. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.7221 mL | 8.6107 mL | 17.2215 mL | |
| 5 mM | 0.3444 mL | 1.7221 mL | 3.4443 mL | |
| 10 mM | 0.1722 mL | 0.8611 mL | 1.7221 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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