| Size | Price | Stock | Qty |
|---|---|---|---|
| 50U |
|
||
| Other Sizes |
| Targets |
CMP-sialic acid synthetase targets sialic acid (N-acylneuraminate) and CTP as substrates. It catalyzes the reversible reaction: CTP + N-acylneuraminate ⇌ diphosphate + CMP-N-acylneuraminate. It activates free sialic acid by converting it to CMP-sialic acid, the donor substrate for all sialyltransferases. It does not have a pharmacological target but is an enzymatic tool.
|
|---|---|
| ln Vitro |
In vitro, CMP-sialic acid synthetase catalyzes the formation of CMP-sialic acid and its derivatives. It has an activity of >1000 U/mL or >10 units/mg protein. One unit is defined as the amount of enzyme that catalyzes the formation of 1 μmol of CMP-sialic acid per minute at 37°C and pH 8.0.
|
| ln Vivo |
In vivo, CMP-sialic acid synthetase is essential for the biosynthesis of sialic acid-containing carbohydrates and glycoconjugates. It is involved in aminosugars metabolism. It is not used as a therapeutic agent but is a research enzyme. It is used in glycobiology research for synthesizing sialylated glycans.
|
| Enzyme Assay |
Cell-free enzyme assays for CMP-sialic acid synthetase: the enzyme is incubated with sialic acid, CTP, and varying concentrations of inhibitors or modulators in assay buffer (pH 8.0) at 37°C. The reaction is stopped, and the formation of CMP-sialic acid is measured by HPLC, mass spectrometry, or coupled enzyme assays. Enzyme activity (U/mg) is calculated.
|
| Cell Assay |
In vitro cellular assays for CMP-sialic acid synthetase: cells are not typically treated with this enzyme as it is an intracellular enzyme. The enzyme is used in cell-free systems for glycan synthesis. In cellular studies, the enzyme's role in sialylation is studied by knocking down or overexpressing the enzyme and measuring cell surface sialylation by lectin staining or mass spectrometry.
|
| Animal Protocol |
In vivo animal studies for CMP-sialic acid synthetase: the enzyme is not administered to animals as a therapeutic agent. It is a research reagent. No specific pharmacological studies are reported. Animal models of sialic acid metabolism disorders could be used to study the enzyme's role.
|
| ADME/Pharmacokinetics |
Pharmacokinetic properties of CMP-sialic acid synthetase: as an enzyme, it is not intended for systemic administration. No systemic pharmacokinetics are applicable. The enzyme is supplied as a lyophilized powder containing Tris-HCl and NaCl. It is stored at -20°C.
|
| Toxicity/Toxicokinetics |
Toxicity of CMP-sialic acid synthetase: as an enzyme, it is generally well-tolerated. In research settings, the enzyme is considered safe for laboratory use. Standard laboratory safety precautions should be followed. The product is not intended for human therapeutic use.
|
| References | |
| Additional Infomation |
CMP-sialic acid synthetase is a research enzyme used in glycobiology for the synthesis of sialic acid-containing carbohydrates and glycoconjugates. It is available as a recombinant enzyme from E. coli. Its primary application is in the synthesis of CMP-sialic acid and its derivatives for biochemical and structural studies. No clinical applications have been reported.
|
| CAS # |
9067-82-7
|
|---|---|
| Appearance |
Colorless to light yellow liquid
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
