| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| 100mg |
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| Other Sizes |
| Targets |
Spirostan-3-ol does not have a specific biological target for therapeutic applications. It functions as a bee repellent, keeping bees away from areas recently treated with pesticides. Its mechanism is not well characterized but may involve olfactory or gustatory deterrent effects.
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|---|---|
| ln Vitro |
In vitro, Spirostan-3-ol is a steroidal sapogenin with potential applications as a bee repellent. Detailed in vitro activity data, including IC50 values, are not reported. It is a naturally occurring compound.
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| ln Vivo |
In vivo, Spirostan-3-ol is used as a tool to keep bees away from areas recently treated with toxic insecticides. It has potential applications in agriculture and pest management. No therapeutic applications are reported.
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| Enzyme Assay |
Cell-free assays for Spirostan-3-ol: as a natural product, its characterization includes determination of purity by HPLC and structural confirmation by NMR and mass spectrometry. Bee repellent activity is assessed in behavioral assays where bees are exposed to the compound and their avoidance behavior is measured. No specific biochemical assays are applicable.
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| Cell Assay |
Cellular assays for Spirostan-3-ol: insect cells or olfactory neurons might be used to study its mechanism of action as a repellent. However, no specific cellular assays are reported. It is primarily used as a chemical tool in agricultural research.
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| Animal Protocol |
In vivo animal studies for Spirostan-3-ol: field studies are conducted to evaluate its efficacy as a bee repellent. The compound is applied to areas treated with insecticides, and bee visitation is monitored. No therapeutic animal studies are reported.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties of Spirostan-3-ol: as a lipophilic steroidal sapogenin (MW 416.64), it may have poor oral bioavailability. It is likely metabolized in the liver. Storage: powder at -20°C for up to 3 years; in solvent at -80°C for 6 months or -20°C for 1 month. Purity: 95%.
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| Toxicity/Toxicokinetics |
Toxicity of Spirostan-3-ol: comprehensive toxicity data are limited. As a saponin, it may have hemolytic activity and gastrointestinal effects at high doses. It is for research use only and not approved for human use.
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| References | |
| Additional Infomation |
Spirostan-3-ol is a research compound used as a bee repellent in agricultural research. It is a steroidal sapogenin. No clinical applications have been reported.
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| Molecular Formula |
C27H44O3
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|---|---|
| Molecular Weight |
416.64
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| Exact Mass |
416.329
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| CAS # |
82597-74-8
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| Related CAS # |
Sarsasapogenin;126-19-2
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| PubChem CID |
3035446
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.1±0.1 g/cm3
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| Boiling Point |
516.6±20.0 °C at 760 mmHg
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| Melting Point |
18ºC
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| Flash Point |
266.2±21.8 °C
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| Vapour Pressure |
0.0±3.0 mmHg at 25°C
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| Index of Refraction |
1.552
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| LogP |
6.21
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| Hydrogen Bond Donor Count |
1
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| Hydrogen Bond Acceptor Count |
3
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| Rotatable Bond Count |
0
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| Heavy Atom Count |
30
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| Complexity |
694
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| Defined Atom Stereocenter Count |
9
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| SMILES |
C[C@H]1[C@@H]2[C@]3(CC[C@@H]4[C@]5(CCC(O)CC5CC[C@H]4[C@@H]3C[C@@H]2O[C@]21OCC(C)CC2)C)C
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| InChi Key |
GMBQZIIUCVWOCD-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C27H44O3/c1-16-7-12-27(29-15-16)17(2)24-23(30-27)14-22-20-6-5-18-13-19(28)8-10-25(18,3)21(20)9-11-26(22,24)4/h16-24,28H,5-15H2,1-4H3
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| Chemical Name |
5',7,9,13-tetramethylspiro[5-oxapentacyclo[10.8.0.02,9.04,8.013,18]icosane-6,2'-oxane]-16-ol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.4002 mL | 12.0008 mL | 24.0015 mL | |
| 5 mM | 0.4800 mL | 2.4002 mL | 4.8003 mL | |
| 10 mM | 0.2400 mL | 1.2001 mL | 2.4002 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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