| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
S1R agonist 3 (compound 1) (0.01-10000 nM; 120 min) effectively binds to S1R on the transfected HEK293 cell membrane, with a Ki value of 1.5 ± 0.5 nM[1]. S1R agonist 3 (0.01-1 μM; 30 min) also exhibits potent S1R agonist activity in CHO overexpression cells, inducing S1R-BiP dissociation, with an EC50 value of 160 ± 8 nM[1].
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|---|---|
| ln Vivo |
S1R agonist 3 (compound 1) effectively inhibited the excessive activity of wfs1abKO zebrafish larvae without affecting the normal movement of wild-type larvae [1]. S1R agonist 3 (0.03 mg/kg; intraperitoneal injection; single dose; 20 minutes after Aβ25-35 injection) effectively reversed Aβ25-35-induced learning and memory impairment in mice [1].
|
| Cell Assay |
ELISA detection [1]
Cell Types: Chinese hamster ovary (CHO) cells overexpressing green fluorescent protein (GFP) labeled S1R Tested Concentrations: 0.01-1 μM Incubation Duration: 30 minutes Experimental Results: S1R dissociated from BiP, with an EC50 value of 160 ± 8 nM. This dissociation was blocked by co-treatment with 10 μM S1R antagonist NE-100. |
| Animal Protocol |
Animal/Disease Models: wfs1abKO mutant larvae; wfs1abWT wild-type larvae (4 days post-fertilization) [1]
Doses: 3 μM Route of Administration: Continuous exposure in embryo culture medium for 24 hours Experimental Results: Significantly reduced hypermotility in wfs1abKO larvae, restoring their locomotor behavior to a level comparable to that of wfs1abWT larvae. No significant effect was observed on the locomotor function of wfs1abWT larvae, with a protection index (PI) within the optimal range of 0.8 < PI < 1.2. Animal/Disease Models:Swiss OF1 mice (male, 1 month old; intraventricular injection of oligomeric Aβ25-35 peptide) [1] Doses: 0.03 mg/kg Route of Administration: Intraperitoneal injection; single dose; 20 minutes after Aβ25-35 injection Experimental Results: Spontaneous alternation behavior (an indicator of short-term spatial memory) was significantly improved in mice treated with Aβ25-35. The traversal latency (an indicator of long-term non-spatial memory) was significantly prolonged in mice treated with Aβ25-35, reversing Aβ-induced amnesia. |
| References |
| Molecular Formula |
C14H20CLNO
|
|---|---|
| Molecular Weight |
253.77
|
| CAS # |
401501-66-4
|
| Appearance |
Typically exists as solids at room temperature
|
| SMILES |
ClC1=CC=C(C=C1)OCCN2CCC(CC2)C
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.9406 mL | 19.7029 mL | 39.4058 mL | |
| 5 mM | 0.7881 mL | 3.9406 mL | 7.8812 mL | |
| 10 mM | 0.3941 mL | 1.9703 mL | 3.9406 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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