| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
The combination of pea lectin FITC and Hoechst 33258 can accurately detect the acrosome status and motility of pig and goat sperm, and is an effective method for assessing sperm function [1]. Pea lectin (1-10 μg/mL; 24 h) (FITC) can stimulate the supernatant of untreated C57BL/6 mouse spleen cells and induce nitric oxide production in thioglycolate-induced mouse peritoneal macrophages in a dose-dependent manner [2]. Pea lectin (1-3 μg/mL; 24 h) (FITC) can induce untreated C57BL/6 mouse spleen cells to produce large amounts of IFNγ and TNFα [2]. Pea lectin (3 μg/mL; 24 h) can activate immature CD3+ spleen cells of C57BL/6 mice within 24 hours, upregulate the expression of CD69, downregulate the expression of L-selectin, but do not change the expression of CD122 or VLA-4 [2].
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|---|---|
| ln Vivo |
Pea lectin (200 µg; intravenous injection; single dose) (FITC) can activate CD3+ splenic lymphocytes in male C57BL/6 mice[2].
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| Animal Protocol |
Animal/Disease Models:C57Bl/6 (male, 6-8 weeks old) [2]
Doses: 200 µg Route of Administration: Intravenous injection; single dose Experimental Results: At 24, 48 and 72 hours post-injection, the proliferative response of splenic mononuclear cells to in vitro mitotic stimulation was reduced; lectin-induced proliferation was almost completely blocked, and PMA+ ionomycin-induced proliferation was inhibited at 24 hours. At 24 hours post-injection, the percentage of splenic CD3+ cells increased; the CD4/CD8 ratio remained unchanged. At 24 hours post-injection, the percentage of CD3+ cells expressing activation markers CD69 and CD122 increased. At 24 hours post-injection, L-selectin expression in CD3+ cells decreased, while VLA-4 expression showed no significant change. |
| References |
| Related CAS # |
Pisum Sativum Agglutinin
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|---|---|
| Appearance |
Typically exists as solids at room temperature
|
| SMILES |
[Pisum Sativum Agglutinin (FITC)]
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| Synonyms |
PSA (FITC)
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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