| Size | Price | |
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| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vivo |
Chlorphosphine (2.5–12.5 mg/L; water immersion; 6 hpf to 96 hpf/6 dpf) can induce dose-dependent developmental toxicity, neurotoxicity, oxidative stress and apoptosis in zebrafish embryos, with an LC50 of 8.4 mg/L[1].
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| Animal Protocol |
Animal/Disease Models:Wild-type AB; Tg(sox10:GFP) transgenic (embryos 6 hours post-fertilization) [1]
Doses: 2.5 mg/L, 5 mg/L, 7.5 mg/L, 10 mg/L, 12.5 mg/L Route of Administration: Water immersion; continuous administration; 6 hours to 96 hours post-fertilization (most assays); 6 hours to 6 days post-fertilization (motor analysis) Experimental Results: The 50% lethal concentration (LC50) at 96 hours post-fertilization was determined to be 8.4 mg/L, and the mortality rate increased in a dose-dependent manner. At 96 hours post-fertilization (hpf), at a concentration of 7.5 mg/L, the hatching rate decreased to 76.7%, the average heart rate decreased to 50 beats/20 seconds, and 33.3% of the embryos developed pericardial edema. At 96 hours post-fertilization (hpf), at concentrations of 2.5–7.5 mg/L, the fluorescence intensity of sox10-GFP in Tg(sox10:GFP) embryos decreased, ventricles enlarged, intercellular spaces increased, and the number of new neurons decreased. At 6 days post-fertilization (dpf), at concentrations of 2.5–7.5 mg/L, total motor distance, motor time, average speed, motor frequency, and maximum acceleration all decreased in a dose-dependent manner. At 6 days post-fertilization (dpf), at a concentration of 7.5 mg/L, acetylcholinesterase (AChE) activity was 1.0 U/mg protein. At a concentration of 7.5 mg/L, the expression of neurodevelopmental genes was altered: syn2a (0.33-fold decrease compared to the control group), gfap (0.32-fold decrease), elavl3 (3.2-fold decrease), neurog (0.59-fold decrease), gap43 (0.64-fold decrease), and sox19b (1.41-fold decrease). At a concentration of 7.5 mg/L, the fluorescence intensity of reactive oxygen species (ROS) was significantly increased; the activities of superoxide dismutase (SOD), malondialdehyde (MDA), and catalase (CAT) decreased to 0.68, 0.55, and 0.64 times that of the control group, respectively. At a concentration of 7.5 mg/L, p53 protein levels increased, Bax expression was enhanced, and the Bax/Bcl2 ratio increased; acridine orange (AO) staining showed that at a concentration of 7.5 mg/L, brain cell apoptosis increased. |
| References |
| Molecular Formula |
C12H14CLN2O3PS
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|---|---|
| Molecular Weight |
332.74
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| CAS # |
14816-20-7
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| Appearance |
Typically exists as solids at room temperature
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| SMILES |
N#CC(=NOP(=S)(OCC)OCC)C=1C=CC=CC1Cl
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.0053 mL | 15.0267 mL | 30.0535 mL | |
| 5 mM | 0.6011 mL | 3.0053 mL | 6.0107 mL | |
| 10 mM | 0.3005 mL | 1.5027 mL | 3.0053 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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