| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
BMS-869780 alone does not inhibit the production of total Aβ, but when used in combination with acidic GSM (GSM-2 or GSM-1) in human glioma H4-APPsw cells, it exhibits a strong synergistic inhibitory effect on the production of Aβ1-x and Aβ1-40 (combination index values as low as 0.05-0.06) and can induce the accumulation of α-CTF [1].
|
|---|---|
| ln Vivo |
BMS-869780 (25 mg/kg; oral; single dose) significantly reduced brain Aβ1-42 levels at 3 and 6 hours after administration to wild-type C57Bl/6 mice, but had no significant effect on total brain Aβ1-x levels [1].
|
| Animal Protocol |
Animal/Disease Models:C57Bl/6 (12-week-old males/females, wild type)[1]
Doses: 25 mg/kg Route of Administration: Oral; Single dose Experimental Results: 3 hours after administration, brain Aβ1-42 levels were significantly lower than in the solvent control group. 3 hours after administration, brain Aβ1-x levels were not significantly different from those in the solvent control group. 6 hours after administration, brain Aβ1-42 levels were still significantly lower than those in the solvent control group. 6 hours after administration, brain Aβ1-x levels were not significantly different from those in the solvent control group. 3 or 6 hours after administration, no significant synergistic reduction in brain Aβ1-x levels was observed when combined with GSM-2. Compared with BMS-869780 alone, Aβ1-42 levels were slightly significantly lower 6 hours after administration when combined with GSM-2. |
| References |
| Molecular Formula |
C23H22CLFN6O
|
|---|---|
| Molecular Weight |
452.92
|
| CAS # |
1235493-78-3
|
| Appearance |
Typically exists as solids at room temperature
|
| SMILES |
N(C=1N=C2[C@@H](CCCCN2N1)C3=CC=C(F)C=C3)C4=CC(OC)=C(C=C4)N5C=NC(Cl)=C5
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.2079 mL | 11.0395 mL | 22.0790 mL | |
| 5 mM | 0.4416 mL | 2.2079 mL | 4.4158 mL | |
| 10 mM | 0.2208 mL | 1.1039 mL | 2.2079 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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