| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Antiviral agent 83 (compound 13B) (10 μM; 48 hours) effectively inhibited HPV-16 PsVs infection in HeLa cells with low cytotoxicity, with a viral inhibition rate of 83.33% and a cytotoxicity of 5.71% [1]. Antiviral agent 83 (48 hours) showed an IC50 of 8.84 μM for HPV-16 PsVs inhibition and low cytotoxicity in both HeLa and VK2/E6E7 cells, with CC50 values of 62.14 μM and 89.08 μM, respectively, and selectivity indices of 7.03 and 10.08, respectively [1]. Antiviral agent 83 (0.625-10 μM; 48 hours) effectively inhibited the infection of high-risk HPV-16, -18, -31 and -45 pseudoviruses in HeLa cells, with IC50 values ranging from 7.80 to 12.45 μM [1]. Antiviral agent 83 (5-20 μM; pre-incubation for 1 hour or 4 hours; total incubation for 48 hours) can directly inactivate HPV-45 pseudovirus in a dose- and time-dependent manner, with a stronger inhibitory effect the longer the pretreatment time [1]. Antiviral agent 83 (10 μM; 4 hours) can destroy the morphological integrity of HPV-45 pseudovirus after incubation at a concentration of 10 μM for 4 hours [1]. Antiviral agent 83 can specifically and with high affinity bind to HPV-16 L1 protein, with a Kd of 93.6 nM [1].
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|---|---|
| ln Vivo |
Antiviral agent 83 (20-40 μM; intravaginal; daily; 3 days) provided almost complete protection against HPV-16 PsV infection in the genitals of nude mice and significantly inhibited viral signaling [1].
|
| Cell Assay |
Cytotoxicity assay [1]
Cell Types: HeLa cells infected with HPV. Tested Concentrations: 0.625, 1.25, 2.5, 5, 8, 10 μM Incubation Duration: 48 hours Experimental Results: The IC50 values for HPV-16, -18, -31 and -45 pseudovirus strains in HeLa cells were 8.84, 8.31, 12.45 and 7.8 μM, respectively. |
| Animal Protocol |
Animal/Disease Models:HPV-infected nude mice (female, 6–8 weeks old) [1]
Doses: 20 μM; 40 μM Route of Administration: Intravaginal injection; daily; 3 days Experimental Results: From day 2, luciferase signal in the vagina of mice decreased. At a concentration of 40 μM, only a very weak luciferase signal was observed in the vagina of all mice on day 4. |
| References |
| Molecular Formula |
C29H25BN4O2
|
|---|---|
| Molecular Weight |
472.35
|
| Appearance |
Typically exists as solids at room temperature
|
| SMILES |
CN1C2=NC3=CC=CC=C3C2=C(NC4=CC(NCC5=CC(B(O)O)=CC=C5)=CC=C4)C6=C1C=CC=C6
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1171 mL | 10.5854 mL | 21.1707 mL | |
| 5 mM | 0.4234 mL | 2.1171 mL | 4.2341 mL | |
| 10 mM | 0.2117 mL | 1.0585 mL | 2.1171 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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