| Size | Price | Stock | Qty |
|---|---|---|---|
| 50mg |
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| 100mg |
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| Other Sizes |
| ln Vitro |
Guidelines (The following is our recommended operating procedure. This procedure is for reference only and should be modified according to your specific needs.) 1. Preparation of Alizarin Red S[1] 1.1 Prepare a 2% sodium alizarin red S aqueous solution. 1.2 Using a pH meter, add ammonium hydroxide dropwise with stirring to adjust the pH to 4.2. 1.3 Filter the solution through a 0.45 μm microporous membrane and store it in a brown bottle. 1.4 Filter the solution again through a 0.22 μm microporous membrane immediately before use. 2. Alizarin Red S detection of osteoblast differentiation[2] 2.1 Osteogenic differentiation induction: MC3T3-E1 cells were seeded in well plates and cultured in α-MEM medium (without ascorbic acid) supplemented with 10% fetal bovine serum. Osteogenic differentiation was induced by osteogenic medium (OSM) containing ascorbic acid (50 μg/mL) and sodium β-glycerophosphate (10 mM). 2.2 Staining: MC3T3-E1 cells were washed with 1×PBS, fixed with 70% ethanol for 10 minutes at room temperature, rinsed with distilled water, and then stained with Alizarin Red S for 10 minutes to detect calcium deposition in the matrix. 2.3 Data analysis: The area of the red region was positively correlated with the degree of osteogenic differentiation. 3. Screening of calcium compounds in synovial fluid with Alizarin Red S [1] 3.1 Synovial fluid preparation: Synovial fluid was drawn with a disposable syringe and the liquid was transferred to a clean test tube containing heparin sodium anticoagulant. 3.2 Staining: A drop of synovial fluid was taken from the bottom of the test tube, dropped onto a clean glass slide, a drop of 2% Alizarin Red S solution was added, mixed well, and covered with a coverslip. 3.3 Within three minutes after mixing, the cells were observed under a regular optical microscope, and the degree of orange-red staining observed in each high-power field was graded. The area of the red region was proportional to the content of calcium compounds.
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| ln Vivo |
In vivo staining of bones with Alizarin Red S[3] (1) Rat treatment: 20% Alizarin Red S solution (100 mg/kg, single dose) was injected into albino rats, and the rats were sacrificed 2 minutes to 8 hours after injection. (2) Skull treatment: The rat skull was cut off and soaked in 2% potassium hydroxide solution for several days. All soft tissues were removed, and after separation, it was rinsed with tap water and stored in 80% ethanol. The skull was embedded in unsaturated polyester resin and cut into 50-60 μm thick sections along the sagittal or coronal plane with a diamond saw. (3) Microscopic examination: Under 80x magnification, the bones emitted green autofluorescence, and Alizarin Red S emitted red fluorescence.
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| References |
| Molecular Formula |
C14H8O7S
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|---|---|
| Molecular Weight |
320.27
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| CAS # |
83-61-4
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| Related CAS # |
Alizarin Red S sodium; Alizarin Red S Indicator (4.3-6.3), IND
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| Appearance |
Yellow to brown solid
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| SMILES |
O=S(C(C=C1C2=O)=C(O)C(O)=C1C(C3=C2C=CC=C3)=O)(O)=O
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| Synonyms |
ARS
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~312.24 mM; with sonication)
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1224 mL | 15.6118 mL | 31.2237 mL | |
| 5 mM | 0.6245 mL | 3.1224 mL | 6.2447 mL | |
| 10 mM | 0.3122 mL | 1.5612 mL | 3.1224 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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