| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Not applicable. 2-(1-Methylethyl)phenyl diphenyl phosphate is a flame retardant. It does not have a specific biological receptor target for therapeutic purposes. In environmental toxicology, it is studied as an endocrine disruptor. It may bind weakly to the estrogen receptor (ER) and androgen receptor (AR), but with very low potency (IC50 > 50 uM). It is an inhibitor of acetylcholinesterase at very high concentrations, but this is not its target. Its primary function is chemical (flame retardant).
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| ln Vitro |
In vitro, this compound is used as an analytical standard (HPLC, GC-MS) for the detection of flame retardants. For cytotoxicity screening (e.g., HepG2 cells), the IC50 is typically >50 uM (MTT assay). It may induce oxidative stress at high concentrations. It is not used in enzyme activity assays for drug discovery.
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| ln Vivo |
In vivo, this compound is not a drug. It is not used for therapeutic efficacy. It is studied as an environmental contaminant. In a 28-day oral study in rats (100 mg/kg/day), it may cause slight body weight loss and liver vacuolation. It is used to study the bioaccumulation of OPFRs.
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| Enzyme Assay |
General protocol for in vitro analytical use: For GC-MS analysis, extract water or soil samples with dichloromethane. Prepare calibration standards in hexane (0.1-10 ug/mL). Inject 1 uL into a DB-5 column (30 m). Oven program: 80degC to 300degC. Use EI-MS detection (SIM mode, m/z 251, 326, 367). For LC-MS/MS, use a C18 column with water:acetonitrile (0.1% formic acid), positive ion mode, m/z 369 → 251. The compound is stored at 2-8degC.
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| Cell Assay |
General protocol for in vitro cell-based experiments: For androgen receptor antagonism, use MDA-kb2 cells. Treat with 1 nM DHT and varying concentrations of the compound (1-100 uM). 2-(1-Methylethyl)phenyl diphenyl phosphate may inhibit 30-50% of DHT-induced luciferase activity at 100 uM (weak antagonist).
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| Animal Protocol |
General protocol for in vivo animal experiments: For a toxicology study, administer the compound (100 mg/kg, oral, daily) to rats for 28 days. Monitor weight and liver enzymes. The compound may induce CYP2B/2E1 activity (based on literature for similar OPFRs).
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| ADME/Pharmacokinetics |
General pharmacokinetic properties: This is a lipophilic liquid (LogP ~4.5, MW 368.36). It is stable in water. It is metabolized by CYP450 to hydroxylated metabolites. For storage, keep at room temperature in a sealed container.
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| Toxicity/Toxicokinetics |
General toxicity profile: It is an irritant. It is toxic to aquatic life. The oral LD50 in rats is >2000 mg/kg. Standard safety precautions for organophosphates (gloves, lab coat) should be used.
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| Additional Infomation |
This compound is a component of commercial flame retardant mixtures (e.g., isopropylated triphenyl phosphate, IPTPP). It is for research use only.
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| Molecular Formula |
C21H21O4P
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|---|---|
| Molecular Weight |
368.36
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| CAS # |
64532-94-1
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| Appearance |
Typically exists as solids at room temperature
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.7147 mL | 13.5737 mL | 27.1474 mL | |
| 5 mM | 0.5429 mL | 2.7147 mL | 5.4295 mL | |
| 10 mM | 0.2715 mL | 1.3574 mL | 2.7147 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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