| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
|
||
| 10mg |
|
||
| Other Sizes |
| Targets |
This compound is a chemical linker and has no biological target. It is used for the site-specific conjugation of biomolecules (e.g., antibodies, peptides, DNA) in vitro. The TCO group undergoes a rapid, bioorthogonal iEDDA reaction with tetrazine groups (rate constant k2 > 1000 M-1s-1). This reaction is highly specific and occurs under physiological conditions without metal catalysts. The PEG3 spacer provides solubility and reduces steric hindrance. The amine group serves as a handle for conjugation to activated carboxyl groups (NHS esters) of biomolecules.
|
|---|---|
| ln Vitro |
In vitro, TCO-PEG3-NH2 is not used in biological activity assays. It is used in bioconjugation chemistry. For example, to label an antibody with a tetrazine dye, the antibody is first reacted with TCO-PEG3-NH2 via EDC/NHS to introduce TCO groups. Then, the TCO-modified antibody is reacted with a tetrazine-fluorophore (e.g., Cy5-Tetrazine) to produce the final labeled antibody. The reaction is monitored by LC-MS or SDS-PAGE (shift). There is no direct cell-based activity.
|
| ln Vivo |
Not applicable (a chemical linker, not a therapeutic). TCO-PEG3-NH2 is not administered in vivo. It is used to prepare imaging probes or targeted drug delivery systems. For example, an antibody conjugated to TCO-PEG3-NH2 can be pre-injected into a mouse, followed by injection of a tetrazine-labeled toxin or radionuclide for "pretargeted" imaging or therapy. This approach is used in cancer research. The linker itself is not dosed.
|
| Enzyme Assay |
General protocol for in vitro chemical conjugation: For conjugation to a protein, dissolve the protein (1 mg/mL) in PBS (pH 7.4). Add EDC (1 mM) and NHS (2 mM) and stir for 15 min. Add TCO-PEG3-NH2 (100 uM, 10× molar excess). Incubate at RT for 2 h. Dialyze against PBS to remove unreacted linker. To test click reaction, incubate TCO-modified protein (10 uM) with tetrazine-Cy5 (10 uM) in PBS for 1 h. Analyze by SDS-PAGE (fluorescence scanning) or LC-MS. For analytical purposes, use HPLC (C18 column, water/acetonitrile gradient, UV 210 nm). Store TCO-PEG3-NH2 as a powder at -20degC. TCO is light-sensitive; protect from light. Stock solutions in DMSO (50 mM) can be stored at -80degC for 6 months.
|
| Cell Assay |
General protocol for in vitro cell-based experiments: Not applicable for biological activity. For live-cell labeling, if a protein is pre-labeled with TCO and tetrazine dye, the reaction occurs on the cell surface. However, the linker itself is not added to cells.
|
| Animal Protocol |
General protocol for in vivo animal experiments: For pretargeted imaging, inject mice with TCO-modified antibody (100 ug, IV). After 24-48 h to allow tumor accumulation, inject tetrazine-radiolabeled probe (e.g., 68Ga-tetrazine, IV). Image by PET after 1-2 h. TCO-PEG3-NH2 is not injected alone.
|
| ADME/Pharmacokinetics |
General pharmacokinetic properties: TCO-PEG3-NH2 is a small molecule (MW 330.42). It is not a drug. It is soluble in water, DMSO, and DMF. For storage, keep as a powder at -20degC (for up to 2 years). Avoid moisture and light (TCO group is light-sensitive). Working solutions in water can be stored at 4degC for 1 day.
|
| Toxicity/Toxicokinetics |
General toxicity profile: TCO-PEG3-NH2 is a chemical reagent. It may be an irritant. Standard safety precautions for handling organic chemicals (gloves, lab coat) should be used. It is not a controlled substance. For research use only.
|
| Additional Infomation |
TCO-PEG3-NH2 is a key tool for bioorthogonal chemistry (iEDDA). It is used in pretargeted imaging, drug delivery, and cell surface engineering. For research use only.
|
| Molecular Formula |
C17H32N2O5
|
|---|---|
| Molecular Weight |
344.45
|
| Related CAS # |
(S)-TCO-PEG3-NH2
|
| Appearance |
Oil
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9032 mL | 14.5159 mL | 29.0318 mL | |
| 5 mM | 0.5806 mL | 2.9032 mL | 5.8064 mL | |
| 10 mM | 0.2903 mL | 1.4516 mL | 2.9032 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
