| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Ivospemin hydrochloride (500 nM to 10 µM, 96 h) reduced the cell viability of human ovarian adenocarcinoma cells, with IC50 values ranging from 0.95 µM to 3.24 µM, independent of cisplatin sensitivity [2]. IC50 values of human ovarian adenocarcinoma cell lines after Ivospemin treatment [1] Cell Line CaOV-3 A2780 OV90 ACRP Ivospemin 96 h IC50 2.56 µM 0.95 µM 3.24 µM 1.41 µM
|
|---|---|
| ln Vivo |
Ivospemin hydrochloride (24 mg/kg, intraperitoneal injection, once every 2 weeks for 3 consecutive weeks, alternating weeks for 60 days) increased survival in a mouse model of ovarian adenocarcinoma by delaying disease effects and reducing overall tumor burden [2][3].
|
| Cell Assay |
Cell Viability Assay[2]
Cell Types: A2780, OV90, ACRP, and CaOV-3 cell lines Tested Concentrations: 500 nM to 10 µM Incubation Duration: 96 h Experimental Results: Reduced cell viability in A2780, OV90, ACRP, and CaOV-3 cell lines. |
| Animal Protocol |
Animal/Disease Models: Murine Ovarian Adenocarcinoma Model[2][3]
Doses: 24 mg/kg Route of Administration: i.p., 2qw×3, alternating weeks for 60 days Experimental Results: Produced a 20% increase in median survival from 43 days to 52 days post-injection, extended the time to ascites formation to 46.9 days, an increase of 13% combined with Gemcitabine or Topotecan. |
| References | |
| Additional Infomation |
Ivospemin Hydrochloride is the hydrochloride form of Ivospemin, an analogue of the natural polyamine (PA) with potential antitumor activity. After subcutaneous injection, Ivospemin displaces endogenous PA from its binding sites on the cell surface, thereby preventing PA internalization. This inhibits PA-dependent cell cycle progression, leading to cell cycle arrest, induction of apoptosis, and inhibition of tumor cell proliferation. PA uptake is upregulated in various tumor types, and elevated PA levels result in enhanced tumor cell growth.
|
| Molecular Formula |
C16H42CL4N4O2
|
|---|---|
| Molecular Weight |
464.34
|
| Exact Mass |
464.203
|
| CAS # |
259657-09-5
|
| Related CAS # |
Ivospemin
|
| PubChem CID |
155676458
|
| Appearance |
Typically exists as solids at room temperature
|
| Hydrogen Bond Donor Count |
10
|
| Rotatable Bond Count |
17
|
| Heavy Atom Count |
26
|
| Complexity |
198
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
CCNCC[C@@H](CNCCCCNC[C@H](CCNCC)O)O.Cl.Cl.Cl.Cl
|
| InChi Key |
SCDBHUCANIJZED-VNMXSFETSA-N
|
| InChi Code |
InChI=1S/C16H38N4O2.4ClH/c1-3-17-11-7-15(21)13-19-9-5-6-10-20-14-16(22)8-12-18-4-2;;;;/h15-22H,3-14H2,1-2H3;4*1H/t15-,16-;;;;/m0..../s1
|
| Chemical Name |
(2S)-4-(ethylamino)-1-[4-[[(2S)-4-(ethylamino)-2-hydroxybutyl]amino]butylamino]butan-2-ol;tetrahydrochloride
|
| Synonyms |
SBP-101 hydrochloride
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.1536 mL | 10.7680 mL | 21.5359 mL | |
| 5 mM | 0.4307 mL | 2.1536 mL | 4.3072 mL | |
| 10 mM | 0.2154 mL | 1.0768 mL | 2.1536 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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