| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
This PROTAC linker does not have a direct biological target for therapeutic intervention. Its function is to serve as a chemical bridge, providing a stable framework to connect a ligand for an E3 ubiquitin ligase (such as cereblon or VHL) and a ligand for a target protein of interest. The primary chemical target is the two reactive moieties (the amine group can form amides or carbamates, while the hydroxyl can be activated for nucleophilic substitution) to which it conjugates during PROTAC synthesis.
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| ln Vitro |
PROTAC contains two distinct ligands linked by a single linker: one is the ligand for the E190 ubiquitin ligase, and the other is the ligand for the target protein. PROTAC utilizes the intracellular ubiquitin-proteasome system to selectively degrade the target protein.
As a PROTAC linker, 2-(4-(Aminomethyl)piperidin-1-yl)ethanol itself lacks direct biological activity in cell-free systems. It is not an enzyme inhibitor or receptor ligand. Any observed activity would be derived from the complete PROTAC molecule, not the linker alone. For typical enzymatic assays (e.g., kinase activity), this compound is inert and would show no inhibition at concentrations up to 100 uM. |
| ln Vivo |
The compound is not used as a therapeutic agent in vivo. Its in vivo role is confined to being a component of a larger PROTAC molecule. When incorporated into a PROTAC, the conjugate may exhibit activity in animal models of disease (e.g., tumor xenografts). The linker itself, if administered alone, would likely be rapidly cleared and show no efficacy. There are no published reports on the direct in vivo activity of this specific linker.
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| Enzyme Assay |
Not applicable. 2-(4-(Aminomethyl)piperidin-1-yl)ethanol is not evaluated in enzymatic or receptor binding assays. For analytical characterization, purity is assessed by HPLC using a C18 column with a mobile phase of acetonitrile/water (containing 0.1% TFA) and detection by UV at 210 nm or by ELSD. Mass spectrometry (ESI-MS) is used to confirm identity, looking for the [M+H]+ ion at m/z 159.25.
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| Cell Assay |
Not applicable. This PROTAC linker is not used as a test article in cell-based assays. It may be used as a negative control in PROTAC experiments. In such a control, cells are treated with the linker alone (0.1-10 microM) for 48-72 hours, and cell viability is assessed by MTT or CellTiter-Glo. The compound typically shows no significant cytotoxicity or protein degradation activity at these concentrations, confirming that any observed effects from a complete PROTAC are due to target engagement.
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| Animal Protocol |
Not applicable. This compound is a synthetic building block and is not administered to animals for efficacy or pharmacokinetic studies as a standalone agent. It is stored in a cool, dry place under standard laboratory conditions. It is handled with standard precautions for organic compounds (gloves, lab coat, eye protection) and is intended for research use only, not for human consumption.
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| ADME/Pharmacokinetics |
Not applicable. 2-(4-(Aminomethyl)piperidin-1-yl)ethanol is a PROTAC linker, not a drug candidate. It has no pharmacological PK properties. If it were absorbed, it would behave as a piperidine derivative, likely undergoing phase II metabolism (glucuronidation) and renal excretion. Its use is strictly confined to in vitro synthesis where it is conjugated to other moieties.
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| Toxicity/Toxicokinetics |
2-(4-(Aminomethyl)piperidin-1-yl)ethanol is generally considered a low-toxicity chemical for laboratory use. The amino group may cause mild skin or eye irritation upon direct contact. No specific target organ toxicity (e.g., hepatotoxicity, nephrotoxicity) has been reported. Standard chemical handling procedures should be followed: use of safety goggles, nitrile gloves, and a laboratory coat in a well-ventilated area. It has CAS number 21168-72-9. Its molecular formula is C8H18N2O, and its molecular weight is 158.25 g/mol. The IUPAC name is 2-[4-(aminomethyl)piperidin-1-yl]ethanol. It typically appears as a colorless to light yellow liquid. It is soluble in DMSO and water. It is stable under normal storage conditions (powder at -20degC for up to 3 years, in solvent at -80degC for 6 months) and requires protection from light.
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| Additional Infomation |
CAS: 21168‑72‑9. Formula C₈H1₈N2O, MW 158.25. IUPAC: 2‑[4‑(aminomethyl)piperidin‑1‑yl]ethanol. Colorless to light yellow liquid. Soluble in DMSO and water. Store powder at ‑20 degC (3 years), in solvent at ‑80 degC (6 months). For research only.
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| Molecular Formula |
C8H18N2O
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|---|---|
| Molecular Weight |
158.25
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| Exact Mass |
158.142
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| CAS # |
21168-72-9
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| PubChem CID |
7330476
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| Appearance |
Colorless to light yellow liquid
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| Hydrogen Bond Donor Count |
2
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
11
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| Complexity |
100
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1CN(CCC1CN)CCO
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| InChi Key |
VKMYXOQRUXXUHE-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C8H18N2O/c9-7-8-1-3-10(4-2-8)5-6-11/h8,11H,1-7,9H2
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| Chemical Name |
2-[4-(aminomethyl)piperidin-1-yl]ethanol
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 6.3191 mL | 31.5956 mL | 63.1912 mL | |
| 5 mM | 1.2638 mL | 6.3191 mL | 12.6382 mL | |
| 10 mM | 0.6319 mL | 3.1596 mL | 6.3191 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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