| Size | Price | Stock | Qty |
|---|---|---|---|
| 100g |
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| Other Sizes |
| ln Vitro |
Ammonium acetate is usually present as white crystals. It is usually prepared from acetic acid and ammonia water, neutralized to pH 7.0. It is widely used in analytical chemistry, medicine, textile printing and dyeing and other fields.
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|---|---|
| Toxicity/Toxicokinetics |
Interactions
One hour before administering ammonium acetate to dogs with ECK fistulas, administration of an equimolar concentration of a mixture of ornithine and aspartic acid (1 mmol/kg) effectively prevented ammonia toxicity in the blood. In rats, the addition of other amino acids in combination with arginine hydrochloride significantly enhanced the protective effect of arginine hydrochloride against ammonium acetate poisoning. Carbamoyl aspartic acid protected rats from the toxic effects of ammonium acetate alone by lowering blood ammonia levels and increasing blood urea levels. Injection of a mixture of 1 mmol/kg N-carbamoyl-L-glutamate and 1 mmol/kg L-arginine provided 100% protection against hyperammonemia in rats, followed by administration of the rat LD99.9 dose (10.8 mmol/kg) of ammonium acetate. For more complete data on ammonium acetate interactions (7 in total), please visit the HSDB record page. |
| Additional Infomation |
Ammonium acetate is a white crystalline solid. Its main hazard lies in its environmental threat. Immediate measures should be taken to limit its environmental release. It is used in chemical analysis, pharmaceuticals, food preservation, and other applications. Ammonium acetate is an ammonium salt produced by the reaction of ammonia and acetic acid. It is a deliquescent white crystalline solid with a relatively low melting point (114°C). It was once used as a food acidity regulator, but the EU no longer approves its use for this purpose. It can be used as both a buffer and a food acidity regulator. It is both an ammonium salt and an acetate. See also: Calcium sulfate (related); Aluminum chloride (related); Talc (related)... See more...
Mechanism of Action Although the exact mechanism of systemic ammonia poisoning remains to be elucidated, evidence suggests that cerebral oxidative energy metabolism is disrupted. In rats induced into a coma by ammonium acetate, basal brain structural samples showed significant reductions in glycogen, glucose, ATP, and especially creatine phosphate. Using cortical cuvette and multi-ion detection techniques, the effect of ammonium ions on glutamate release in the rat cerebral cortex was measured in vivo. The results showed that ammonium ions can increase the release and production of glutamate in the brain. The increased concentration of this amino acid in the extracellular space may be one of the mechanisms of ammonia toxicity in vivo. /Ammonium ions/ Therapeutic Uses Diuretics Veterinary drugs: As a nutritional supplement Veterinary drugs: Formerly used as diuretics and antipyretics |
| Molecular Formula |
C2H7NO2
|
|---|---|
| Molecular Weight |
77.08
|
| Exact Mass |
77.048
|
| CAS # |
631-61-8
|
| PubChem CID |
517165
|
| Appearance |
White to off-white solid powder
|
| Density |
1.07 g/mL at 20 °C
|
| Boiling Point |
117.1ºC at 760 mmHg
|
| Melting Point |
237.2 °F (USCG, 1999)
; 114 °C
; 114 °C
|
| Flash Point |
136 °C
|
| LogP |
0.415
|
| Hydrogen Bond Donor Count |
1
|
| Hydrogen Bond Acceptor Count |
2
|
| Rotatable Bond Count |
0
|
| Heavy Atom Count |
5
|
| Complexity |
25.5
|
| Defined Atom Stereocenter Count |
0
|
| SMILES |
[O-]C(C([H])([H])[H])=O.[N+]([H])([H])([H])[H]
|
| InChi Key |
USFZMSVCRYTOJT-UHFFFAOYSA-N
|
| InChi Code |
InChI=1S/C2H4O2.H3N/c1-2(3)4;/h1H3,(H,3,4);1H3
|
| Chemical Name |
azanium acetate
|
| Synonyms |
Ammonium acetate, for molecular biology, 98%
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
H2O : ≥ 200 mg/mL (~2594.71 mM)
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 12.9735 mL | 64.8677 mL | 129.7353 mL | |
| 5 mM | 2.5947 mL | 12.9735 mL | 25.9471 mL | |
| 10 mM | 1.2974 mL | 6.4868 mL | 12.9735 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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