| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
Biochemical Assay Reagent
|
|---|---|
| References | |
| Additional Infomation |
Enzymes, coenzymes, and metabolites are typical reagents in enzyme analysis. Some of these substances must be prepared or isolated in the laboratory, but many are available commercially. Information on these substances is more difficult for analysts to obtain compared to that on inorganic reagents. The diversity of purity definitions exacerbates this situation. In 1955, the Committee on Biochemistry and Chemical Technology of the National Research Council of the U.S. National Academy of Sciences began collecting standard values for biochemical compounds. The result of this work was Volume I of Specifications and Standards for Biochemical Compounds, published in June 1960. This volume did not include chapters on "Phosphates" and "Enzymes." This chapter discusses the activity and stability of biochemical reagents. The best standard for enzyme purity is specific activity and details of the activity of contaminating enzymes. The activity of these contaminants is expressed as a percentage of the specific activity of the described enzyme. Enzyme activity is expressed in units, and specific activity is expressed in units per milligram of protein. To conform to the International System of Units (SI), the unit of enzyme activity is defined as the amount of enzyme that converts 1 micromolar of substrate in 1 minute at 25°C. The ADH molecule contains up to 40 thiol groups and 5 zinc atoms, making it sensitive to oxidants and chelating agents. In a 2.0 M (NH4)2SO4 solution containing 3% Na4P2O7 and 1% glycine, the crystalline suspension of ADH can be stably stored for several months in the absence of air at 0°C to 4°C, while it is only stably stored for 1 week at room temperature. The solution or suspension can be dialyzed with distilled water at 0°C to 3°C without significant loss of activity. Triose phosphate isomerase is used as a coenzyme, and glycerol-1-phosphate dehydrogenase is used as an indicator enzyme. [1]
|
| Molecular Formula |
C10H16N2O4
|
|---|---|
| Molecular Weight |
228.25
|
| Exact Mass |
228.111
|
| CAS # |
58477-85-3
|
| PubChem CID |
94074
|
| Appearance |
Typically exists as solids at room temperature
|
| Density |
1.192 g/cm3
|
| Boiling Point |
566.8ºC at 760 mmHg
|
| Melting Point |
186-188 °C(lit.)
|
| Flash Point |
296.6ºC
|
| Index of Refraction |
108 ° (C=2.4, H2O)
|
| LogP |
0
|
| Hydrogen Bond Donor Count |
4
|
| Hydrogen Bond Acceptor Count |
4
|
| Rotatable Bond Count |
7
|
| Heavy Atom Count |
16
|
| Complexity |
250
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
O([H])[C@@]([H])(C(N([H])C([H])([H])C([H])=C([H])[H])=O)[C@@]([H])(C(N([H])C([H])([H])C([H])=C([H])[H])=O)O[H]
|
| InChi Key |
ZRKLEAHGBNDKHM-HTQZYQBOSA-N
|
| InChi Code |
InChI=1S/C10H16N2O4/c1-3-5-11-9(15)7(13)8(14)10(16)12-6-4-2/h3-4,7-8,13-14H,1-2,5-6H2,(H,11,15)(H,12,16)/t7-,8-/m1/s1
|
| Chemical Name |
(2R,3R)-2,3-dihydroxy-N,N'-bis(prop-2-enyl)butanediamide
|
| Synonyms |
58477-85-3; N,N'-Diallyltartardiamide; DTXSID60886266; Butanediamide, 2,3-dihydroxy-N1,N4-di-2-propen-1-yl-, (2R,3R)-; Butanediamide, 2,3-dihydroxy-N,N'-di-2-propenyl-, (R-(R*,R*))-; DTXCID401025601; 261-277-3; N,N'-Diallyl-L-tartardiamide;
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3812 mL | 21.9058 mL | 43.8116 mL | |
| 5 mM | 0.8762 mL | 4.3812 mL | 8.7623 mL | |
| 10 mM | 0.4381 mL | 2.1906 mL | 4.3812 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
