| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| Targets |
Biochemical Assay Reagent
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|---|---|
| References | |
| Additional Infomation |
Enzymes, coenzymes and metabolites are the reagents characteristic of enzymatic analysis. Some of these materials must be prepared or isolated in the laboratory, but many are available commercially. Information about these materials is less readily available to the analyst than information about inorganic reagents. This situation is made worse by the multiplicity and variety of the definitions of purity. In 1955, the Committee on Biological Chemistry and Chemical Technology, National Academy of Sciences, National Research Council (USA) began to collect standard values for biochemical compounds1). As a result of this work the first volume of “Specifications and Criteria of Biochemical Compounds” was published in June 1960*). This volume does not contain chapters on “Phosphate Esters” and “Enzymes”.
This chapter examines the activity and the stability of biochemical reagents. The best criterion for the purity of an enzyme is the specific activity together with details of the activity of contaminating enzymes. The activity of these contaminants is expressed as a percentage of the specific activity of the enzyme described. The activities of the enzymes are given in units and the specific activity in units per mg protein. To conform to the proposed International Unit, a unit of enzyme activity is defined as the amount of enzyme which transforms 1 μmole of substrate in 1 min at 25°C. ADH contains up to 40 SH groups and 5 zinc atoms per molecule and therefore, it is sensitive to oxidizing and chelating agents. The crystalline suspension in 2.0 M (NH4)2SO4 solution containing 3% Na4P2O7 and 1% glycine is stable for several months at 0°C to 4°C when air is excluded, while at room temperature it is only stable for 1 week. Solutions or suspensions can be dialyzed against distilled water at 0°C to 3°C without any significant loss of activity. Triosephosphate isomerase is used as the auxiliary enzyme and glycerol-1-phosphate dehydrogenase as the indicator enzyme.[1]
|
| Molecular Formula |
C10H16N2O4
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|---|---|
| Molecular Weight |
228.25
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| CAS # |
58477-85-3
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| Appearance |
Typically exists as solids at room temperature
|
| SMILES |
O([H])[C@@]([H])(C(N([H])C([H])([H])C([H])=C([H])[H])=O)[C@@]([H])(C(N([H])C([H])([H])C([H])=C([H])[H])=O)O[H]
|
| Synonyms |
58477-85-3; N,N'-Diallyltartardiamide; DTXSID60886266; Butanediamide, 2,3-dihydroxy-N1,N4-di-2-propen-1-yl-, (2R,3R)-; Butanediamide, 2,3-dihydroxy-N,N'-di-2-propenyl-, (R-(R*,R*))-; DTXCID401025601; 261-277-3; N,N'-Diallyl-L-tartardiamide;
|
| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.3812 mL | 21.9058 mL | 43.8116 mL | |
| 5 mM | 0.8762 mL | 4.3812 mL | 8.7623 mL | |
| 10 mM | 0.4381 mL | 2.1906 mL | 4.3812 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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