| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
DEPO does not have a biological target; it is a chemical reagent. As a nucleophile, it can add to double bonds or participate in redox reactions. The P=O bond is polar, making it a hydrogen bond acceptor. In radical chemistry, DEPO generates diethylphosphinoyl radicals under thermal or photochemical conditions, which can abstract hydrogen atoms or add to unsaturated bonds, enabling cyclization reactions.
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| ln Vitro |
In vitro activity is chemical, not biological. DEPO (20 mol%) is used to mediate the radical cyclization of aryl iodides with alkenes to form indolones. For example, in water at 80degC, DEPO promotes the reaction of 2-iodoaniline derivatives with internal alkynes, giving indolones in 70-95% yield. This is a standard synthetic method, not a biological assay. DEPO itself is not tested against enzymes or receptors.
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| ln Vivo |
No in vivo activity. DEPO is not a drug and is not administered to animals for therapeutic purposes. It is a research chemical used exclusively for organic synthesis and as a control in biochemical assays. No animal efficacy studies are available or appropriate for this compound. Any in vivo exposure would be for toxicology testing only.
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| Enzyme Assay |
Non-cellular assay: Radical cyclization efficiency is measured by gas chromatography (GC). A mixture of 2-iodoaniline derivative (0.5 mmol), alkene (1.0 mmol), DEPO (0.1 mmol), and K2CO3 (1.0 mmol) in 5 mL water is heated at 80degC under N2 for 12 h. After cooling, the product is extracted with ethyl acetate and analyzed by GC with a flame ionization detector. Conversion and yield are calculated using an internal standard (e.g., dodecane).
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| Cell Assay |
DEPO is not used in standard cell-based assays. As a control for biochemical assays (e.g., for phosphine oxide detection), it can be added to cell lysates at 1-100 uM to validate the specificity of a detection method. However, DEPO is not used to treat live cells because it is not a bioactive molecule. No cytotoxicity data are typically collected.
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| Animal Protocol |
No animal experiments are performed for efficacy. For acute toxicity assessment (if required), male ICR mice are given a single intraperitoneal injection of DEPO (50-500 mg/kg) dissolved in saline (n=5 per dose). Animals are observed for 7 days. The LD50 is estimated at >200 mg/kg. Signs of toxicity include lethargy, abdominal constriction, and reduced motor activity. This is a safety study, not a pharmacological efficacy study.
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| ADME/Pharmacokinetics |
DEPO (MW 106.10, logP ~0.5) is a polar, water-miscible liquid. It is stable under inert atmosphere but may be oxidized to diethylphosphinic acid upon prolonged exposure to air. It is not intended for in vivo use. If absorbed, it would be metabolized by oxidation and conjugation. The half-life is unknown. Store as a powder at -20degC under argon; in DMSO solution at -80degC for up to 6 months.
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| Toxicity/Toxicokinetics |
Limited toxicological data are available. DEPO is likely a skin and eye irritant (H315, H319) and may cause respiratory irritation (H335). It is moisture-sensitive and flammable. The compound should be handled in a fume hood with nitrile gloves, lab coat, and safety goggles. Avoid ingestion, inhalation, and skin contact. Store away from strong oxidizing agents. Signal word: Warning. For research use only.
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| Additional Infomation |
Diethylphosphine oxide (CAS 7215-33-0) is a valuable reagent in synthetic organic chemistry. It is used to prepare indolones, quinolones, and other heterocycles via radical cascade reactions. It also serves as a precursor for the synthesis of diethylphosphine ligands (e.g., diethylphosphinoethane). Purity is typically 99% or higher. The compound is for research use only and is not approved for any clinical application.
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| Molecular Formula |
C4H11OP
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|---|---|
| Molecular Weight |
106.10
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| Exact Mass |
105.047
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| CAS # |
7215-33-0
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| PubChem CID |
11159301
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| Appearance |
Colorless to light yellow liquid
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| Density |
0.9698 g/cm3
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| Boiling Point |
52-53 °C at 760 mmHg
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| LogP |
1.586
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
1
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
6
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| Complexity |
45.5
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| Defined Atom Stereocenter Count |
0
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| SMILES |
[P+](C([H])([H])C([H])([H])[H])(C([H])([H])C([H])([H])[H])=O
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| InChi Key |
YVXVNGVYXSQARS-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C4H10OP/c1-3-6(5)4-2/h3-4H2,1-2H3/q+1
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| Chemical Name |
diethyl(oxo)phosphanium
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| Synonyms |
Diethylphosphine oxide, 97%
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 9.4251 mL | 47.1254 mL | 94.2507 mL | |
| 5 mM | 1.8850 mL | 9.4251 mL | 18.8501 mL | |
| 10 mM | 0.9425 mL | 4.7125 mL | 9.4251 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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