| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| 50mg |
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| 100mg |
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| Other Sizes |
| Targets |
dCTP is a substrate for DNA polymerases and reverse transcriptases, which incorporate it into the growing DNA chain opposite a guanine (G) base. It acts as a donor of deoxycytidine monophosphate (dCMP) during DNA replication, repair, and PCR. The triphosphate group provides the energy for the phosphodiester bond formation. dCTP is not a drug but an essential reagent for nucleic acid amplification and sequencing technologies.
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| ln Vitro |
In vitro, dCTP (10-200 uM) is used as a substrate in PCR reactions to amplify target DNA sequences. It is also used in DNA sequencing (Sanger sequencing) along with dideoxynucleotides (ddNTPs). dCTP is a component of the dNTP mix for reverse transcription of RNA to cDNA. The purity (>98%) is confirmed by HPLC. The compound is not cytotoxic at concentrations used in PCR (1-1000 uM). It is a biochemical reagent, not a test agent for pharmacological studies.
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| ln Vivo |
Not applicable. dCTP is not administered in vivo as a drug. It is used as a biochemical reagent for in vitro DNA synthesis. In cell culture, it can be added to the medium to support nucleotide pool balance, but it is not a therapeutic agent. dCTP is used in research on nucleotide metabolism and DNA replication. No therapeutic in vivo activity is reported.
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| Enzyme Assay |
Not applicable. dCTP is a nucleotide, not a standard enzyme inhibitor. Its purity (>98%) is assessed by HPLC. The structure is confirmed by ¹H NMR and 3¹P NMR. Physicochemical properties: MW 511.12, white powder, soluble in water (50-100 mg/mL), stored at -20degC. The compound is moisture-sensitive and should be protected from light. For molecular biology applications, it is used as a component of dNTP mixes. For quality control, the concentration is measured by UV absorbance at 260 nm (ε = 1.5×10⁴ M-¹ cm-¹).
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| Cell Assay |
Not applicable. dCTP is not used in standard cell-based assays for drug discovery. For toxicity screening, HepG2 or HEK293 cells are seeded in 96-well plates (1×10⁴ cells/well) and treated with dCTP (1-1000 uM) for 24-72 h. Cell viability is measured by MTT assay. The CC₅0 is expected to be >1000 uM, indicating very low cytotoxicity. The compound is a nucleotide, not a test agent for pharmacological studies. For nucleotide pool analysis, cells are lysed and intracellular dNTPs are extracted and measured by LC-MS/MS.
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| Animal Protocol |
No in vivo animal protocol for dCTP exists, as it is not a drug candidate. For metabolic studies, male Sprague-Dawley rats (200-250 g, n=6/group) are administered a radioactive nucleotide precursor (e.g., [3H]-cytidine), and the incorporation into dCTP pools in tissues is measured. dCTP itself is not administered. The compound is a research reagent, not a test agent for animal studies.
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| ADME/Pharmacokinetics |
No PK data for dCTP is available, as it is not a drug. It is a highly polar, negatively charged molecule that is not passively absorbed. It is used exclusively as an in vitro reagent. For research use, it is stored as a solution at -20degC (protected from light) or as a lyophilized powder at -20degC. Repeated freeze-thaw cycles should be avoided.
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| Toxicity/Toxicokinetics |
For dCTP disodium, hazard statements: H315 (Causes skin irritation), H319 (Causes serious eye irritation), H335 (May cause respiratory irritation). Signal word: Warning. Precautionary statements: P261 (Avoid breathing dust/fume/gas/mist/vapors/spray), P280 (Wear protective gloves/protective clothing/eye protection/face protection), P305+P351+P338 (IF IN EYES: Rinse cautiously with water for several minutes). Storage: at -20degC, sealed, protect from light.
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| Additional Infomation |
dCTP disodium (Deoxycytidine triphosphate disodium; CAS# 102783-51-7) is a research-grade nucleotide reagent for molecular biology. It is not an FDA-approved drug. It is used as a substrate for DNA polymerases in PCR, DNA sequencing, and cDNA synthesis. For research use only, not for diagnostic or therapeutic applications. Purity: ≥98%. Storage: -20degC, sealed, protect from light.
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| Molecular Formula |
C9H14N3NA2O13P3
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|---|---|
| Molecular Weight |
511.12
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| CAS # |
102783-51-7
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| Appearance |
White to off-white solid powder
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| LogP |
0.275
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| SMILES |
P(=O)(O[H])(OP(=O)(O[H])OP(=O)(O[H])O[H])OC([H])([H])[C@]1([H])[C@]([H])(C([H])([H])[C@]([H])(N2C(N=C(C([H])=C2[H])N([H])[H])=O)O1)O[H].[Na].[Na] |^1:44,45|
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| Synonyms |
dCTP disodium; 2′-Deoxycytidine-5′-triphosphate disodium
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage. (2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~125 mg/mL (~244.56 mM; with ultrasonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9565 mL | 9.7824 mL | 19.5649 mL | |
| 5 mM | 0.3913 mL | 1.9565 mL | 3.9130 mL | |
| 10 mM | 0.1956 mL | 0.9782 mL | 1.9565 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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