| Size | Price | Stock | Qty |
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| 1mg |
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| Other Sizes |
| Targets |
Biotinyl-Amylin (mouse, rat) TFA targets the amylin receptor, a complex of the calcitonin receptor (CTR) with receptor activity-modifying proteins (RAMPs). Amylin is a pancreatic hormone co-secreted with insulin. It plays a crucial role in glucose metabolism by regulating gastric emptying, suppressing glucagon secretion, and promoting satiety. The biotinyl group does not interfere with the receptor binding properties of the peptide. In fact, biotinyl analogues of amylin retain a similar affinity for binding to rat liver plasma membranes compared to rat amylin.
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| ln Vitro |
In vitro, Biotinyl-Amylin (mouse, rat) TFA is used as a probe to study amylin receptor binding and expression. Biotinyl analogues of amylin retain similar affinity for binding to rat liver plasma membranes compared to rat amylin. In functional assays, this biotinylated peptide completely inhibits insulin-stimulated glycogen synthesis in rat soleus muscle incubated in vitro, demonstrating that the biotin label does not impair its biological activity. This biotinylated form is ideal for pull-down assays, ELISA, and flow cytometry.
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| ln Vivo |
In vivo, the parent peptide, amylin, is a 37-amino acid pancreatic protein that controls several metabolic events such as glycemia and lacticemia. Biotinyl-Amylin (mouse, rat) TFA is primarily a tool for ex vivo or in vitro applications. It can be used in animal models to track the distribution of amylin or to study its binding to tissues, but this typically requires injection of the labeled peptide. The biotin tag allows for detection with streptavidin-conjugated probes (e.g., HRP, fluorophores) in histochemical or biochemical assays.
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| Enzyme Assay |
The binding affinity of Biotinyl-Amylin for its receptor can be assessed by a competitive binding assay. Rat liver plasma membranes (RLPM) are prepared and incubated with varying concentrations (0.01-1000 nM) of Biotinyl-Amylin TFA in the presence or absence of excess unlabeled amylin. After incubation and washing, the bound Biotinyl-Amylin is detected using a streptavidin-conjugated HRP and a colorimetric substrate (e.g., TMB). The absorbance at 450 nm is measured, and the specific binding is calculated. The dissociation constant (Kd) can be determined from saturation binding experiments.
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| Cell Assay |
Biotinyl-Amylin (mouse, rat) TFA is not used in standard cell viability assays. Instead, it is used for receptor binding studies. Cells expressing the amylin receptor (e.g., NIH-3T3 cells transfected with CTR and RAMP3) are seeded in 24-well plates. The cells are incubated with varying concentrations of Biotinyl-Amylin TFA (0.1-1000 nM) in binding buffer. Non-specific binding is determined in the presence of a 100-fold excess of unlabeled amylin. After incubation and washing, the cells are lysed, and the bound biotinylated peptide is detected by a streptavidin-HRP ELISA. The binding affinity (Kd) is calculated from saturation binding curves.
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| Animal Protocol |
Amylin has been shown to induce hypoglycemia in mice. For in vivo studies, the biotinylated form can be used for histochemical detection. Male C57BL/6 mice (n=6/group) are injected intraperitoneally (IP) or intravenously (IV) with Biotinyl-Amylin (mouse, rat) TFA (10-50 ug). Control groups receive vehicle or unlabeled amylin. At defined intervals, the mice are sacrificed, and tissues (e.g., brain, pancreas, stomach, kidney) are harvested. Tissue sections are prepared and stained with streptavidin-conjugated fluorophores or HRP to visualize the distribution of the injected biotinylated peptide. This allows for the direct mapping of amylin binding sites.
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| ADME/Pharmacokinetics |
Biotinyl-Amylin (mouse, rat) TFA (MW 4146.69) is a peptide. As a biotinylated peptide, it is metabolically stable for limited time periods in vitro, but in vivo it would be subject to rapid proteolytic degradation. The biotin label is not chemically altered by metabolic processes. It is typically stored as a lyophilized powder at -20degC or -80degC. For research use, it is reconstituted in water, PBS, or buffer containing carrier protein (e.g., 0.1% BSA) to prevent adsorption to surfaces. No detailed PK data is available.
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| Toxicity/Toxicokinetics |
Standard safety precautions for handling peptides apply. Biotinyl-Amylin (mouse, rat) TFA is for research use only and is not intended for human consumption. It may cause skin and eye irritation. For handling, use personal protective equipment (gloves, lab coat, eye protection), work in a fume hood, avoid inhalation and skin contact.
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| References | |
| Additional Infomation |
Biotinyl-Amylin (mouse, rat) TFA is a research-grade biotinylated peptide used as a high-affinity probe for amylin receptors. It is not an FDA-approved drug. It is a valuable tool for studying amylin binding, receptor expression, and its role in metabolic diseases such as diabetes and obesity. For research use only, not for diagnostic or therapeutic applications. Storage: powder at -20degC for 3 years, 4degC for 2 years; in solvent at -80degC for 1 year.
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| Molecular Formula |
C177H286N54O55S3.XC2HF3O2
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| Molecular Weight |
4146.69 (free base)
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| Appearance |
Solid powder
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
H2O : ~50 mg/mL (with ultrasonication)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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