| Size | Price | Stock | Qty |
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| 5g |
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| 10g |
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| Other Sizes |
| Targets |
The targets of Protease for flavors are peptide bonds in proteins and polypeptides. As a serine protease, it catalyzes the hydrolysis of peptide bonds, breaking down proteins into smaller peptides and amino acids. This proteolytic activity is harnessed for flavor development and protein modification in food processing applications.
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| ln Vitro |
In vitro, Protease for flavors exhibits proteolytic activity under neutral to alkaline pH conditions. Enzyme engineering focuses on enhancing its reaction kinetics, substrate selectivity, and activity under harsh conditions such as low or high pH. The enzyme's activity is assessed by measuring the release of amino acids or peptides from protein substrates.
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| ln Vivo |
In vivo activity data for Protease for flavors are not applicable, as this enzyme is used in food processing and biocatalyst applications rather than as a therapeutic agent. Its biological activity is evaluated in vitro under controlled conditions that mimic food processing environments. The enzyme is not intended for administration to living organisms for therapeutic purposes.
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| Enzyme Assay |
The non-cellular assay for Protease for flavors involves incubating the enzyme with a protein substrate (e.g., casein or hemoglobin) in an appropriate buffer system at optimal pH and temperature. Proteolytic activity is measured by quantifying the release of amino acids or peptides using spectrophotometric methods (e.g., Folin-Ciocalteu reagent) or by following the decrease in substrate turbidity. Activity is expressed in units per milligram of protein.
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| Cell Assay |
In vitro cell-based assays are not typically performed for Protease for flavors, as it is an enzyme used in food processing rather than a bioactive compound for cellular studies. Its activity is assessed in biochemical assays using purified protein substrates under controlled conditions that simulate food processing environments.
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| Animal Protocol |
In vivo animal study protocols are not applicable for Protease for flavors, as it is not a therapeutic agent. The enzyme is used in food processing applications, including flavor development in high-protein flours used in baking and debittering of protein hydrolysates. Safety assessments for food enzymes follow established regulatory guidelines for food additives.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable for Protease for flavors, as it is not a therapeutic agent administered to living organisms. The enzyme is used in food processing applications. Its stability and activity under processing conditions are the primary considerations rather than absorption, distribution, metabolism, or excretion.
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| Toxicity/Toxicokinetics |
Toxicological data for Protease for flavors are not extensively reported in the available literature. As a food processing enzyme, it is subject to regulatory safety assessments for use in food applications. The enzyme is generally recognized as safe for its intended use in food processing. Standard safety precautions should be observed during handling of the enzyme preparation.
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| References | |
| Additional Infomation |
Protease for flavors (CAS# 37259-58-8) is a serine protease used as a biocatalyst in food processing. It is used to develop savory flavors and to debitter protein hydrolysates, with applications in high-protein flours used in baking. Enzyme engineering enhances its reaction kinetics and substrate selectivity. It is not a therapeutic drug and has no clinical trial or marketing authorization status.
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| CAS # |
37259-58-8
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| Appearance |
Typically exists as solids at room temperature
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| Synonyms |
Flavor protease
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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