| Size | Price | Stock | Qty |
|---|---|---|---|
| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Not applicable. DC4 Crosslinker is a chemical crosslinking reagent for XL-MS, not a drug with a specific biological target. It targets primary amine groups (-NH2) on lysine residues and the N-termini of proteins. The compound reacts covalently with these amines via its two NHS ester groups. The DC4 moiety is a cleavable linker that fragments under collision-induced dissociation (CID) conditions during MS/MS, generating diagnostic ions that enable unambiguous identification of crosslinked peptides.
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| ln Vitro |
DC4 Crosslinker has no direct biological activity; it is a chemical crosslinker used to capture transient or weak protein-protein interactions by covalently linking interacting proteins. The compound is designed with an MS-cleavable DC4 group that enables identification of crosslinked peptides by LC-MS/MS. It is water-soluble, amine-reactive, and has a spacer arm length of approximately 17.5 Angstrom, allowing crosslinking of proteins within this distance. The crosslinker is cell-permeable and can be used for in vivo crosslinking studies. No specific IC50 or activity data is relevant.
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| ln Vivo |
DC4 Crosslinker is not a drug and is not used as a therapeutic agent in vivo. It can be used for in vivo crosslinking studies: the crosslinker can be administered to animals to capture protein-protein interactions in their native cellular environment. After administration, tissues are harvested, proteins are extracted, digested, and crosslinked peptides are analyzed by mass spectrometry. However, specific in vivo data for DC4 Crosslinker is not provided. No efficacy or pharmacological data is reported.
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| Enzyme Assay |
Standard in vitro crosslinking protocol: Purified protein complexes or cell lysates (0.5-2 mg/mL protein) in PBS or HEPES buffer (pH 7.5-8.0) are mixed with DC4 Crosslinker at a final concentration of 0.5-5 mM. The reaction is incubated at 25degC for 30-60 minutes. The crosslinking reaction is quenched by adding 50 mM Tris-HCl (pH 7.5) or 100 mM ammonium bicarbonate and incubating for 15-30 minutes. Crosslinked proteins are reduced (DTT, 10 mM, 30 min), alkylated (iodoacetamide, 20 mM, 30 min), and digested with trypsin (overnight, 37degC). Digested peptides are desalted and analyzed by LC-MS/MS. The DC4 linker fragments under CID, producing diagnostic MS2 patterns.
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| Cell Assay |
Not applicable. DC4 Crosslinker is used in chemical biology to crosslink proteins in cell lysates or intact cells, not as a direct pharmacological modulator. For in vivo crosslinking, cells in culture (e.g., HeLa cells) are treated with DC4 Crosslinker (0.5-5 mM in culture medium) for 30-60 minutes at 37degC. Cells are washed, lysed, and processed for XL-MS analysis as described above. No additional cell-based pharmacological assays are performed with this reagent.
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| Animal Protocol |
Not applicable. DC4 Crosslinker is not intended for standard animal efficacy studies. For in vivo crosslinking studies to map protein-protein interactions in tissues, the crosslinker may be administered to animals (e.g., mice) by intravenous injection (e.g., 10-50 mg/kg). After 30-60 minutes, tissues (e.g., liver, brain) are harvested and processed for XL-MS as described. The crosslinker is used ex vivo or in vivo as a probe to capture interactions, not as a therapeutic agent. No specific protocols are provided for this compound.
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| ADME/Pharmacokinetics |
DC4 Crosslinker is a chemical reagent, not a drug candidate. Pharmacokinetic studies are not performed on crosslinkers. When used in vivo, the crosslinker would be expected to have a short half-life due to rapid reaction with amines in blood and tissues. The NHS ester groups are hydrolyzed in aqueous environments, with a half-life of approximately 1-2 hours in pH 7.5 buffer at 25degC. This rapid hydrolysis limits its distribution. The compound is typically used in ex vivo crosslinking.
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| Toxicity/Toxicokinetics |
DC4 Crosslinker is a chemical crosslinker and should be handled with standard laboratory safety precautions. The NHS ester groups are reactive and can be irritating to skin and eyes. Material safety data recommends handling in a fume hood with appropriate personal protective equipment (gloves, lab coat, safety goggles). No toxicological data is available; the compound is not intended for human consumption. For research use only.
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| References | |
| Additional Infomation |
DC4 Crosslinker (DSS-DC4) is an MS-cleavable, homobifunctional, amine-reactive crosslinker. Spacer arm length: ~17.5 Angstrom. The DC4 cleavable group enables fragmentation during CID, allowing identification of crosslinked peptides by mass spectrometry. Used for studying protein-protein interactions and protein complex topology in XL-MS. For research use only.
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| Molecular Formula |
C22H32BR2N4O8
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|---|---|
| Molecular Weight |
640.32
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| Exact Mass |
640.057
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| CAS # |
1374647-94-5
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| PubChem CID |
129887550
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| Appearance |
White to off-white solid powder
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| LogP |
0
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
12
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| Heavy Atom Count |
36
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| Complexity |
775
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C(CC(=O)ON1C(=O)CCC1=O)C[N+]23CC[N+](CCCC(=O)ON4C(=O)CCC4=O)(CC2)CC3.[Br-].[Br-]
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| InChi Key |
AJWAJFDEYIAPKX-UHFFFAOYSA-L
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| InChi Code |
InChI=1S/C22H32N4O8.2BrH/c27-17-5-6-18(28)23(17)33-21(31)3-1-9-25-11-14-26(15-12-25,16-13-25)10-2-4-22(32)34-24-19(29)7-8-20(24)30;;/h1-16H2;2*1H/q+2;;/p-2
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| Chemical Name |
(2,5-dioxopyrrolidin-1-yl) 4-[4-[4-(2,5-dioxopyrrolidin-1-yl)oxy-4-oxobutyl]-1,4-diazoniabicyclo[2.2.2]octan-1-yl]butanoate dibromide
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.5617 mL | 7.8086 mL | 15.6172 mL | |
| 5 mM | 0.3123 mL | 1.5617 mL | 3.1234 mL | |
| 10 mM | 0.1562 mL | 0.7809 mL | 1.5617 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
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