| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
Not applicable. MTSSL is a chemical probe for EPR spectroscopy, not a drug targeting a biological receptor. Its “target” is the thiol group (-SH) of cysteine residues in proteins. The methanethiosulfonate group reacts specifically with sulfhydryl groups to form a stable disulfide bond. The nitroxide moiety (N-O) acts as a paramagnetic probe, detectable by EPR. It does not exert any pharmacological activity but serves as a biophysical tool to report on local protein structure and dynamics.
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| ln Vitro |
The “activity” of MTSSL is its ability to form a covalent disulfide bond with cysteine residues, enabling EPR distance measurements. It is commonly used to study protein dynamics, interactions, and conformational changes. The nitroxide moiety allows detection of electron spins, providing information about the local environment surrounding the labeled site. The flexible MTSSL linker enables it to adapt to various protein conformations, making it suitable for probing dynamic processes within biomolecules. No direct pharmacological IC50/EC50 data is relevant.
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| ln Vivo |
No specific in vivo data is available for MTSSL. It is a research chemical used in ex vivo or in vitro EPR studies on purified proteins or within isolated cell membranes. It is not intended for in vivo administration as a therapeutic. For in-cell EPR, cells expressing a cysteine-mutant protein are treated with MTSSL (e.g., 0.1-1 mM) to label the protein, after which cells are lysed, and EPR measurements are performed. Labeling occurs ex vivo on isolated protein or membrane preparations.
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| Enzyme Assay |
Standard site-directed spin labeling protocol: Purify the cysteine-containing protein of interest (typically with engineered cysteine residues at specific sites). Incubate the protein (10-50 uM) with 10- to 50-fold molar excess of MTSSL in buffer (20 mM HEPES pH 7.5, 150 mM NaCl) for 2-16 hours at 4degC or room temperature. Remove excess unbound label by gel filtration or dialysis. The labeled protein is analyzed by EPR spectroscopy. For distance measurements, two cysteine residues are labeled; dipolar coupling data are collected using pulsed EPR (DEER/PELDOR). The distance distribution is calculated from the dipolar evolution.
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| Cell Assay |
Not applicable. MTSSL is used to label proteins purified from cells, not as a treatment for live cells in pharmacological assays. For in-cell labeling, cells expressing the target protein are harvested and lysed. The lysate is incubated with MTSSL (0.1-1 mM) for 1-2 hours at 4degC. The labeled protein is purified (e.g., by affinity chromatography) and then analyzed by EPR. The labeling reaction occurs in cell lysate, not in live cells. No cellular viability or functional assays are performed with MTSSL.
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| Animal Protocol |
Not applicable. MTSSL is not administered to animals for efficacy studies. For ex vivo tissue labeling, animals are euthanized, and tissues expressing the target protein are harvested and homogenized. MTSSL is added to the tissue homogenate (0.1-1 mM, 1-2 hours, 4degC). The labeled protein is purified from the homogenate and analyzed by EPR. No animals are treated with MTSSL in vivo for therapeutic or pharmacological studies. Its use is strictly ex vivo for structural biology.
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| ADME/Pharmacokinetics |
Not applicable. MTSSL is a paramagnetic spin label and research chemical, not a drug candidate. Pharmacokinetic studies are not performed. It is a small molecule (MW 264.38) with a reactive methanethiosulfonate group that hydrolyzes in aqueous solution. It is typically prepared fresh in DMSO or acetonitrile and used immediately for protein labeling. Storage at -20degC under inert atmosphere is recommended. It is not intended for systemic administration.
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| Toxicity/Toxicokinetics |
MTSSL is a chemical probe and should be handled with standard laboratory safety precautions. It is a potential skin and eye irritant. The nitroxide radical is stable but can be reduced by biological reductants such as ascorbate. Avoid inhalation, ingestion, and skin contact. Use gloves, goggles, and lab coat. Work in a fume hood when handling powder. For research use only, not for human or veterinary consumption. Disposal should comply with local regulations.
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| References | |
| Additional Infomation |
MTSSL (CAS: 81213-52-7) has a molecular formula of C10H18NO3S2 and a molecular weight of 264.38. It appears as an orange to red solid. Purity ≥95%. Synonyms: MTSL, MTS, MTSSL, Otmpmms. Solubility: Soluble in DMSO, DMF, acetonitrile, and methanol (typically 10-50 mM). Storage: -20degC, protect from light. It is a widely used spin label in SDSL-EPR for studying protein structure and dynamics. For research use only.
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| Molecular Formula |
C10H18NO3S2
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|---|---|
| Molecular Weight |
264.38
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| Exact Mass |
264.073
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| CAS # |
81213-52-7
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| PubChem CID |
133628
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| Appearance |
Typically exists as solids at room temperature
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| Melting Point |
107-108ºC
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| LogP |
2.843
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| Hydrogen Bond Donor Count |
0
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| Rotatable Bond Count |
3
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| Heavy Atom Count |
16
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| Complexity |
404
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| Defined Atom Stereocenter Count |
0
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| SMILES |
C1(CSS(=O)(=O)C)=CC(C)(C)N([O])C1(C)C |^1:12|
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| InChi Key |
BLSCGBLQCTWVPO-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C10H18NO3S2/c1-9(2)6-8(7-15-16(5,13)14)10(3,4)11(9)12/h6H,7H2,1-5H3
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| Synonyms |
Otmpmms
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.7824 mL | 18.9122 mL | 37.8243 mL | |
| 5 mM | 0.7565 mL | 3.7824 mL | 7.5649 mL | |
| 10 mM | 0.3782 mL | 1.8912 mL | 3.7824 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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