| Size | Price | |
|---|---|---|
| 500mg | ||
| 1g | ||
| Other Sizes |
| ln Vitro |
Spectinomycin sulfate hydrate selectively inhibits protein synthesis in cells and E. coli extracts Spectinomycin sulfate hydrate (50 μg/mL) rapidly and reversibly inhibits E. coli growth and immediately inhibits amino acid incorporation [1]. Spectinomycin sulfate hydrate (1 μg/mL or 3 μM) inhibits peptide synthesis directed by endogenous messenger RNA or MS-2 phage RNA, with a maximum inhibition of 70-80% in E. coli extracts [1]. Spectinomycin sulfate hydrate prevents the translocation of peptidyl tRNA from the A site to the P site of elongation factor G to the ribosome by inhibiting the binding [2]. Spectinomycin sulfate hydrate specifically interacts with residues G1064 and 01192 in 16S rRNA and may inactivate it [2]. Spectinomycin sulfate hydrate exhibits a splicing inhibitory effect that is dependent on pH changes and Mg2+ concentration, suggesting an electrostatic interaction with intronic RNA [3].
|
|---|---|
| ln Vivo |
Spectinomycin sulfate hydrate (20 mg/kg; im; 20-100 mg/kg; 9 d) was shown to be safe in healthy chickens[4]. The major elimination route of spectinomycin sulfate hydrate (10 mg/kg; iv; single dose) is renal excretion, with approximately 55% excreted unchanged in the urine[5]. Pharmacokinetic analysis[5] Parameter C0 (μg/mL) AUC0-∞ (μg•h/mL) Vd (L/kg) CL (L/h/kg) MRT (h) T1/2α (h) T1/2β (h) T1/2γ (h) fe CLrenal (L/h/kg) Eratio Non atrioventricular analysis 44.3 16.8 0.756 0.602 0.757 / / / 0.553 0.359 1.00 Three-compartment model 37.8 15.7 0.747 0.649 1.11 / 0.237 0.754 19.5 / /
|
| Animal Protocol |
Animal/Disease Models: Arbor Acres plus broiler chicks (15-day-old)[4]
Doses: 20 mg/kg, 60 mg/kg, 100 mg/kg Route of Administration: Intramuscular injection (chest muscles); 9 days Experimental Results: Showed biosecurity of 20 mg/kg by complete blood count, biochemical parameters, histopathological, clinical signs, body weight gain, and feed conversion ratio (FCR). Resulted minor toxicity of 60 mg/kg. |
| References | |
| Additional Infomation |
Spectinomycin Sulfate Tetrahydrate is the tetrahydrate form of spectinomycin, an aminocyclic aminoglycoside antibiotic derived from *Streptomyces spectabilis*, which possesses antibacterial activity. Spectinomycin binds to the 30S ribosomal subunit of bacteria, thereby interfering with the initiation of protein synthesis and normal protein elongation, ultimately leading to bacterial death.
See also: Spectinomycin (including the active fraction)... View more... |
| Molecular Formula |
C14H34N2O15S
|
|---|---|
| Molecular Weight |
502.49
|
| Exact Mass |
502.168
|
| CAS # |
64058-48-6
|
| PubChem CID |
64770
|
| Appearance |
Typically exists as solids at room temperature
|
| Boiling Point |
583.1ºC at 760 mmHg
|
| Melting Point |
~185° (dec)
|
| LogP |
0
|
| Hydrogen Bond Donor Count |
11
|
| Hydrogen Bond Acceptor Count |
17
|
| Rotatable Bond Count |
2
|
| Heavy Atom Count |
32
|
| Complexity |
559
|
| Defined Atom Stereocenter Count |
9
|
| SMILES |
OS(O)(=O)=O.CN[C@@H]1[C@H](O)[C@H](NC)[C@@H]2[C@H](O[C@H]3[C@@H](O2)C(=O)C[C@@H](C)O3)[C@H]1O.O.O.O.O
|
| InChi Key |
OBZDRKHRQYPQDZ-SACNDDTHSA-N
|
| InChi Code |
InChI=1S/C14H24N2O7.H2O4S.4H2O/c1-5-4-6(17)14(20)13(21-5)22-12-10(19)7(15-2)9(18)8(16-3)11(12)23-14;1-5(2,3)4;;;;/h5,7-13,15-16,18-20H,4H2,1-3H3;(H2,1,2,3,4);4*1H2/t5-,7-,8+,9+,10+,11-,12-,13+,14+;;;;;/m1...../s1
|
| Chemical Name |
sulfuric acid;(1R,3S,5R,8R,10R,11S,12S,13R,14S)-8,12,14-trihydroxy-5-methyl-11,13-bis(methylamino)-2,4,9-trioxatricyclo[8.4.0.03,8]tetradecan-7-one;tetrahydrate
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9901 mL | 9.9504 mL | 19.9009 mL | |
| 5 mM | 0.3980 mL | 1.9901 mL | 3.9802 mL | |
| 10 mM | 0.1990 mL | 0.9950 mL | 1.9901 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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