| Size | Price | Stock | Qty |
|---|---|---|---|
| 1mg |
|
||
| Other Sizes |
| Targets |
Sphinganine-C17 is an analytical internal standard. It does not have a biological "target" as a drug would. Its mechanism is to serve as an inert chemical standard in mass spectrometry. Because it is structurally analogous to endogenous sphinganine but has a distinct mass shift due to the C17 chain, it co-elutes during liquid chromatography but can be specifically detected via MS. This corrects for sample preparation and instrumental variability, allowing for accurate quantification.
|
|---|---|
| ln Vitro |
Sphinganine-C17 is not a drug and has no biological activity. It is chemically inert in the context of the enzymatic assays it is used to standardize. Its in vitro function is to act as a reference point to measure the activity of enzymes that metabolize endogenous sphingolipids. For example, it can be used to measure the utilization of dihydrosphingosine in resistant leukemia cells by tracking the consumption of the endogenous compound relative to the stable C17 standard.
|
| ln Vivo |
The compound has no direct in vivo activity. It is not administered as a therapeutic drug. In research, it can be spiked into an animal tissue homogenate (e.g., liver, brain) before extraction to quantify the levels of endogenous sphingolipids present in that tissue. This "spike-in" approach provides a snapshot of lipid metabolism in the animal model, but the C17 standard itself does not affect the animal's biology.
|
| Enzyme Assay |
A non-cellular biochemical assay is not applicable. Instead, this compound is used to calibrate instruments. A solution containing a known concentration (e.g., 10-100 ng/mL) of Sphinganine-C17 is prepared in the LC-MS mobile phase. The instrument measures the signal intensity (peak area) of the deuterated standard. This signal is used to create a calibration curve to back-calculate the concentration of endogenous sphinganine in extracted samples. It serves as the sole analytical reagent in the MS setup.
|
| Cell Assay |
For a targeted metabolomics study, cells (e.g., leukemia cells) are lysed, and proteins are precipitated with organic solvents. A fixed amount of Sphinganine-C17 (e.g., 1 pmol/10⁶ cells) is spiked into the lysate. The sample is analyzed by LC-MS/MS. The MS detects the C17 standard (mass shift) and endogenous C18 sphinganine. The ratio of the endogenous sphinganine signal to the C17 standard signal is used to calculate the absolute concentration of the endogenous lipid.
|
| Animal Protocol |
In a preclinical study to assess drug efficacy, a mouse xenograft model of leukemia is treated with a sphingolipid metabolism-targeting drug. After treatment, tumor tissue is harvested. A known quantity of Sphinganine-C17 is added to the tumor homogenate before LC-MS analysis. The concentration of endogenous sphinganine is quantified by comparing it to this internal standard. This allows researchers to measure the pharmacodynamic effect of the drug on the sphingolipid pathway in vivo.
|
| ADME/Pharmacokinetics |
The compound is not a drug and is not designed for in vivo administration. As a reference standard, it is considered stable in storage. It must be stored as a powder at -20degC, protected from light. It is soluble in organic solvents like ethanol, methanol, and DMSO, but is poorly soluble in water. Its pharmacokinetics as a dosing agent is irrelevant.
|
| Toxicity/Toxicokinetics |
Sphinganine-C17 is a highly pure (>99%) chemical standard. It is non-toxic at the trace concentrations used in analytical chemistry. However, standard laboratory safety procedures should be followed. It is a mild skin and eye irritant. Always use gloves and a lab coat. It is not intended for human consumption. It is for research use only.
|
| References | |
| Additional Infomation |
C17 Sphinganine is an aminodiol and sphingosine.
Sphinganine-C17 (d17:0) is an internal standard for sphingolipid analysis by LC-MS. Sphingolipids are essential components of cell membranes and are involved in cell signaling. The C17 analog serves as a gold-standard internal standard for absolute quantification in metabolomics. It is superior to chemical analogs because it corrects for all steps of sample processing. It is used as an internal standard for chromatographic analysis of sphingosine compounds. |
| Molecular Formula |
C17H37NO2
|
|---|---|
| Molecular Weight |
287.48
|
| Exact Mass |
287.282
|
| CAS # |
32164-02-6
|
| PubChem CID |
3247037
|
| Appearance |
Typically exists as solids at room temperature
|
| LogP |
4.458
|
| Hydrogen Bond Donor Count |
3
|
| Hydrogen Bond Acceptor Count |
3
|
| Rotatable Bond Count |
15
|
| Heavy Atom Count |
20
|
| Complexity |
188
|
| Defined Atom Stereocenter Count |
2
|
| SMILES |
CCCCCCCCCCCCCC[C@H]([C@H](CO)N)O
|
| InChi Key |
KFQUQCFJDMSIJF-DLBZAZTESA-N
|
| InChi Code |
InChI=1S/C17H37NO2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14-17(20)16(18)15-19/h16-17,19-20H,2-15,18H2,1H3/t16-,17+/m0/s1
|
| Chemical Name |
(2S,3R)-2-aminoheptadecane-1,3-diol
|
| Synonyms |
Heptadecasphinganine
|
| HS Tariff Code |
2934.99.9001
|
| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
|
| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
|
|---|---|
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.4785 mL | 17.3925 mL | 34.7850 mL | |
| 5 mM | 0.6957 mL | 3.4785 mL | 6.9570 mL | |
| 10 mM | 0.3479 mL | 1.7393 mL | 3.4785 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Products are for research use only; We do not sell to patients
Copyright 2020 InvivoChem LLC | All Rights Reserved
