| Size | Price | Stock | Qty |
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| 5mg |
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| 10mg |
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| Other Sizes |
| Targets |
SBB-Analogue (GL13) Biotin does not target a specific protein in the traditional sense. Instead, it binds selectively to the lipofuscin pigment, which accumulates in the lysosomes of senescent cells. The compound consists of a Sudan Black B (SBB) derivative conjugated to biotin. The SBB portion enables robust detection of senescent cells, while the biotin tag allows for signal amplification using streptavidin-based detection systems. This design effectively detects senescent cells and eliminates false positive staining caused by serum starvation and cell fusion.
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| ln Vitro |
In non-cell assays, SBB-Analogue (GL13) Biotin is used to detect and visualize senescent cells in fixed tissue sections or cell culture preparations. The biotin tag allows for standard immunohistochemistry protocols. Streptavidin conjugated to a fluorophore (e.g., Streptavidin-FITC, -Cy3) or an enzyme (e.g., HRP, AP) is added, which binds to the biotin. The signal is then amplified and visualized by fluorescence microscopy or chromogenic detection.
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| ln Vivo |
In cell-based assays, SBB-Analogue (GL13) Biotin is used to identify and quantify senescent cells. Cells are cultured, fixed, and permeabilized. The compound is applied, and after washing, a streptavidin-conjugated detection reagent is added. The signal is then measured by flow cytometry for quantitative analysis of the senescent cell population. It is also used in immunofluorescence analysis to visualize senescent cells within a culture or tissue section via confocal microscopy.
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| Enzyme Assay |
For a non-cell imaging protocol, cells grown on coverslips or fixed tissue sections are first washed with PBS. They are then incubated with SBB-Analogue (GL13) Biotin in a blocking buffer (e.g., 1% BSA in PBS) for 1 hour at room temperature. After washing, Streptavidin-FITC or Streptavidin-HRP is added and incubated for 30 minutes. After a final wash, coverslips are mounted with DAPI-containing mounting medium and visualized by fluorescence microscopy or light microscopy after chromogenic development.
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| Cell Assay |
For cell-based assays, a 12-well plate of cells is fixed with 4% paraformaldehyde and permeabilized. The cells are incubated with SBB-Analogue (GL13) Biotin solution for 1 hour at room temperature. After washing, Streptavidin-Alexa Fluor 488 is added and incubated for 30 minutes in the dark. The cells are then trypsinized, resuspended in FACS buffer, and analyzed by flow cytometry. The percentage of senescent cells is determined by the increase in fluorescence intensity in the FITC channel.
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| Animal Protocol |
No in vivo animal studies have been published using SBB-Analogue (GL13) Biotin as an injectable agent. The compound is not typically used as a systemic agent but rather as a staining reagent for fixed tissues collected from animal studies. For example, in a mouse model of aging or chemotherapy-induced senescence, tissue sections (e.g., liver, kidney, lung) can be stained with this compound to assess the level of cellular senescence as an endpoint.
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| ADME/Pharmacokinetics |
Pharmacokinetic data is not relevant for SBB-Analogue (GL13) Biotin as it is not administered to animals for in vivo testing. It is a histological and cytological stain. For research use, it is stored as a powder at -20degC for up to three years, and in solution at -80degC for up to one year, protected from light. The compound is soluble in DMSO, which is used to prepare stock solutions.
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| Toxicity/Toxicokinetics |
Toxicity data for SBB-Analogue (GL13) Biotin is not available. It is a staining reagent and is not intended for in vivo administration. As a research chemical, it should be handled with standard laboratory precautions. It may be an irritant to the skin, eyes, and respiratory tract. Appropriate PPE (gloves, lab coat, safety goggles) should be worn.
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| References | |
| Additional Infomation |
SBB-Analogue (GL13) Biotin is a research reagent and is not an FDA-approved drug for human therapeutic or diagnostic use. It is a high-performance chemical tool for detecting cellular senescence, a process implicated in aging, cancer, and various age-related diseases. It is a critical reagent for validating the efficacy of senolytic drugs and for studying the biological role of senescent cells in disease models.
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| Molecular Formula |
C39H38N8O3S
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|---|---|
| Molecular Weight |
698.84
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| CAS # |
2171036-89-6
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| Appearance |
Typically exists as solids at room temperature
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| Synonyms |
GL13; SBB-AB
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.4309 mL | 7.1547 mL | 14.3094 mL | |
| 5 mM | 0.2862 mL | 1.4309 mL | 2.8619 mL | |
| 10 mM | 0.1431 mL | 0.7155 mL | 1.4309 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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