| Size | Price | Stock | Qty |
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| 10mg |
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| Targets |
Purinergic receptors (ATP receptors), specifically P2Y receptors. Procion Blue HB (Reactive Blue 2) acts as an antagonist at P2Y4 receptors and other P2Y subtypes. It is used as an ATP receptor antagonist in research to study purinergic signaling.
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| ln Vitro |
Reactive Blue 2 works as an ATP receptor antagonist and produces Ca2+ oscillations in HeLa cells. Reactive Blue 2 boosts Ca2+ responses to histamine associated with the PLC cascade. Reactive Blue 2 may activate the PLC cascade and cause Ca2+ oscillations in an extracellular Ca2+-dependent way [1]. Application of Reactive Blue 2 enhances K+ secretion in a dose-dependent manner, this rise is characterized by a peak followed by partial relaxation to steady state. Reactive Blue 2 has antagonistic activity at P2Y4, and the antagonistic potency at P2Y4 reflects the potency in K+ secretion [2]. The anthraquinone dye Reactive Blue 2 was discovered to be a strong inhibitor of protein kinases isolated and purified from thylakoids. The inhibition method is non-competitive with a Ki of 8 µM for the membrane-bound kinase and 6 µM for the pure enzyme. The inhibitor does not alter the substrate preference of the endogenous kinase and can be removed from the membrane by washing [3]. Reactive Blue 2 preferentially inhibits processes mediated through P2y purinergic receptors, at least within a restricted dose range. In preparations that enhance tone with norepinephrine, ATP and 2-methylthioATP (but not α,β-methylene ATP) cause vasorelaxation. These relaxations are suppressed in the presence of reactive blue 2 [4]. Reactive Blue 2 suppresses ATP-induced oscillations in a concentration-dependent manner at doses of 0.3-10 μM [5].
Reactive Blue 2 is used as an ATP receptor antagonist and induces Ca2+ oscillations in HeLa cells. It enhances the Ca2+ response to histamine that is linked to the PLC cascade and may activate the PLC cascade in an extracellular Ca2+-dependent manner. It increases K+ secretion in a dose-dependent manner and is a potent inhibitor of a protein kinase isolated from thylakoids. |
| ln Vivo |
No specific in vivo activity data are documented for Procion Blue HB in the provided sources. As a dye and research tool, its primary application is in in vitro biochemical and cell-based assays. It is not typically used for in vivo studies.
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| Enzyme Assay |
In vitro assays for Procion Blue HB are performed to study its effects on purinergic signaling. The compound is used as an antagonist in receptor binding assays with radiolabeled ATP or other purinergic ligands. Its effects on intracellular calcium levels can be measured using fluorescent calcium indicators in cell-based assays. Its inhibitory activity against protein kinases can be assessed using kinase activity assays.
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| Cell Assay |
In vitro cell-based assays for Procion Blue HB are performed to study its effects on purinergic signaling and calcium homeostasis. Cells such as HeLa cells are treated with the compound, and intracellular calcium levels are measured using fluorescent indicators like Fura-2 or Fluo-4. The compound's ability to block ATP-induced responses can also be assessed.
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| Animal Protocol |
In vivo animal studies are not typically conducted with Procion Blue HB. It is a dye and research tool used primarily in vitro. Its use in vivo would be limited due to its properties as a dye and its potential for staining tissues.
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| ADME/Pharmacokinetics |
Procion Blue HB has a molecular weight of 774.16 and a molecular formula of C29H20ClN7O11S3. It is soluble in DMSO at ≥110 mg/mL (142.09 mM) and in water at 10 mg/mL (12.92 mM). It is insoluble in ethanol. It should be stored at 4°C, protected from light. In solution, it can be stored at -80°C for 6 months or at -20°C for 1 month, protected from light.
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| Toxicity/Toxicokinetics |
No specific toxicity data are documented for Procion Blue HB in the provided sources. As a research compound, it is intended for laboratory use only and is not approved for human therapeutic applications. Standard laboratory safety practices, including the use of personal protective equipment, should be followed when handling this compound.
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| References |
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| Additional Infomation |
Procion Blue HB is also known as Reactive Blue 2. It is a triazine dye that can remove viral particles from plasma protein solutions. It is a purinergic antagonist used as an ATP receptor antagonist. It is a dark blue salt suspension with a density of 1.845 g/cm3. It is used in textile and biological staining applications.
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| Molecular Formula |
C29H20N7O11S3CL
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| Molecular Weight |
774.157400000001
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| Exact Mass |
773.007
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| CAS # |
12236-82-7
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| Appearance |
Light brown to black solid powder
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| Density |
1.845 g/cm3
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| Melting Point |
>300ºC
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| Index of Refraction |
1.777
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| LogP |
7.896
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ≥ 110 mg/mL (~142.09 mM)
H2O : ~10 mg/mL (~12.92 mM) Ethanol : ~1 mg/mL (~1.29 mM) |
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.2917 mL | 6.4586 mL | 12.9172 mL | |
| 5 mM | 0.2583 mL | 1.2917 mL | 2.5834 mL | |
| 10 mM | 0.1292 mL | 0.6459 mL | 1.2917 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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