| Size | Price | Stock | Qty |
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| 100mg |
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| 500mg |
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| 1g |
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| Other Sizes |
| Targets |
pNNP is a substrate for various phosphatases, including protein tyrosine phosphatases, alkaline phosphatases, and acid phosphatases. It is cleaved by these enzymes, releasing the chromogenic product 4-nitrophenol. It does not target a specific biological receptor but is used as a tool to measure enzyme activity. It also inhibits β-carbonic anhydrase and α-carbonate dehydrogenase.
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| ln Vitro |
In vitro, pNNP is used as a substrate in colorimetric assays to measure the activity of phosphatases. The hydrolysis of pNNP by phosphatases results in a yellow color that can be quantified spectrophotometrically at 405 nm. It is also used as a substrate for the PP2C assay. The rate of color development is proportional to enzyme activity.
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| ln Vivo |
In vivo, pNNP is not used as a therapeutic agent. Its primary applications are in in vitro biochemical assays for measuring enzyme activity. It is not administered to living organisms for therapeutic purposes. It has no in vivo pharmacological activity.
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| Enzyme Assay |
In vitro enzyme assays for pNNP typically involve incubating the substrate with a phosphatase enzyme (e.g., alkaline phosphatase) in a suitable buffer and measuring the increase in absorbance at 405 nm over time. The assay is used to characterize enzyme kinetics, inhibitor screening, or to measure enzyme activity in biological samples.
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| Cell Assay |
In vitro cellular assays for pNNP are not typically performed, as it is a substrate for biochemical assays rather than a compound with direct effects on cells. However, it can be used in cell-based assays to measure cellular phosphatase activity if the enzyme is released into the culture medium or if cell lysates are used.
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| Animal Protocol |
In vivo animal models are not applicable for pNNP, as it is a substrate for in vitro biochemical assays and not a therapeutic agent. It is not used in animal models for therapeutic purposes, and no in vivo efficacy data exist.
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| ADME/Pharmacokinetics |
pNNP has a molecular formula of C6H6NO6P and a molecular weight of 219.09 g/mol. Its chemical name is 4-nitrophenyl dihydrogen phosphate. It is also known as para-nitrophenyl phosphate. The compound is soluble in water and is typically stored as a powder at room temperature. It is a chromogenic substrate for phosphatases.
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| Toxicity/Toxicokinetics |
pNNP is a chromogenic substrate for phosphatases and is considered a relatively safe laboratory reagent. As with any chemical, appropriate safety precautions should be followed when handling it. It is not intended for human therapeutic use and has low acute toxicity.
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| References |
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| Additional Infomation |
4-Nitrophenyl phosphate is an aryl phosphate ester formed by the monoesterification of phosphoric acid with 4-nitrophenol. It is a mouse metabolite functionally related to 4-nitrophenol and is the conjugate acid of 4-nitrophenyl phosphate (2-). 4-Nitrophenyl phosphate is a metabolite found or produced in Escherichia coli (K12 strain, MG1655 strain). There are reports of the presence of 4-nitrophenyl phosphate in humans, with relevant data. 4-Nitrophenyl phosphate is a metabolite found or produced in Saccharomyces cerevisiae.
pNNP, also known as para-nitrophenyl phosphate, is a chromogenic substrate used to detect and quantify phosphatase enzyme activity. Upon hydrolysis, it produces a yellow color measurable at 405 nm. The compound is for research use only and is not approved for human therapeutic use. |
| Molecular Formula |
C6H6NO6P
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|---|---|
| Molecular Weight |
219.08
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| CAS # |
330-13-2
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| Related CAS # |
32348-90-6 (magnesium salt);32348-91-7 (di-ammonium salt);4264-83-9 (di-hydrochloride salt);54306-27-3 (hydrochloride salt)
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| PubChem CID |
378
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| Appearance |
Typically exists as solid at room temperature
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| Density |
1.712g/cm3
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| Boiling Point |
457.8ºC at 760mmHg
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| Flash Point |
230.7ºC
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| LogP |
0.3
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| Hydrogen Bond Donor Count |
2
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| Hydrogen Bond Acceptor Count |
6
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
14
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| Complexity |
250
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| Defined Atom Stereocenter Count |
0
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| SMILES |
O=P(O)(OC1=CC=C([N+]([O-])=O)C=C1)O
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| InChi Key |
XZKIHKMTEMTJQX-UHFFFAOYSA-N
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| InChi Code |
InChI=1S/C6H6NO6P/c8-7(9)5-1-3-6(4-2-5)13-14(10,11)12/h1-4H,(H2,10,11,12)
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| Chemical Name |
(4-nitrophenyl) dihydrogen phosphate
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| Synonyms |
p NNP; p-NNP; pNNP
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 4.5645 mL | 22.8227 mL | 45.6454 mL | |
| 5 mM | 0.9129 mL | 4.5645 mL | 9.1291 mL | |
| 10 mM | 0.4565 mL | 2.2823 mL | 4.5645 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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