Size | Price | Stock | Qty |
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50mg |
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100mg |
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250mg |
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500mg |
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1g |
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Other Sizes |
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Purity: ≥98%
Pinocembrin (DL-0108), the racemate of Pinocembrin, is a potent androgen receptor ligand with the potential for the treatment of acute stroke. Pinocembrin [(+)-Pinocoembrin; Dihydrochrysin; Galangin flavanone] is a naturally occurring flavonoid that is present in fingerroot, propolis, damiana, and honey. It has strong antimicrobial, anti-inflammatory, and gastrointestinal protective properties. It is a potent anti-allergic agent that functions as a competitive inhibitor of histidine decarboxylase.
ln Vitro |
Pinocembrin (5,7-dihydroxyflavanone) is one of the main flavonoids that have been isolated and purified using different chromatographic techniques from a variety of plants, primarily from Sparattosperma leucanthum, Eucalyptus, Populus, and Pinus heartwood, among other diverse flora. Numerous pharmacological properties, such as antimicrobial, anti-inflammatory, antioxidant, and anticancer properties, have been thoroughly studied[1]. In primary cortical neurons exposed to oxygen-glucose deprivation/reoxygenation (OGD/R), pinocembrin has been demonstrated to increase neuronal viability, decrease lactate dehydrogenase release, inhibit the production of NO and ROS, increase glutathione levels, and downregulate the expression of neuronal NO synthase (nNOS) and iNOS[2].
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ln Vivo |
Pinocembrin has a broad therapeutic time window for its neuroprotective effects against cerebral ischemia injury, which may be related to its antiexcitotoxic properties[1]. It can control apoptosis, modify mitochondrial function, shield the blood-brain barrier, and lower reactive oxygen species (ROS). Astrocytic end-feet edema, neuronal apoptosis, deformation of endothelial cells and capillaries, and brain swelling may all be ameliorated by pinocembrin (10 mg/kg, i.v.). Pinocembrin inhibits ER stress and apoptosis in MCAO rats by lowering the expression of caspase-12 and C/EBP homologous protein (CHOP)/GADD153 through the PERK-elF2α-ATF4 signaling pathway. In mice, the lethal dose of intravenous pinocembrin (LD50) exceeds 700 mg/kg.
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Cell Assay |
SH-SY5Y cells are cultured at 37°C in a humidified environment with 5% CO2 in DMEM supplemented with 10% FBS, 100 units/mL penicillin, and 100 µg/mL streptomycin. Trypsinization is used to pass cells every three to five days. Subsequently, a 96-well plate is seeded with 1 × 104 cells per well, and the plate is incubated for 24 hours at 37 °C in a humidified atmosphere that contains 5% CO2. After one of the compounds is pretreated for one hour, the entire medium is replaced with fresh medium containing 1% FBS. Either 30 nM staurosporine or 2.0 µg/mL tunicamycin is then added. Assays for nuclear staining are conducted 24 hours after the initial incubation. Cell death is evaluated.
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Animal Protocol |
male Sprague-Dawley rats
22.5 or 67.5 mg/kg i.v. |
References |
Molecular Formula |
C15H12O4
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Molecular Weight |
256.25
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Exact Mass |
256.07
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Elemental Analysis |
C, 70.31; H, 4.72; O, 24.97
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CAS # |
68745-38-0
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Related CAS # |
68745-38-0
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Appearance |
Solid powder
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SMILES |
C1C(OC2=CC(=CC(=C2C1=O)O)O)C3=CC=CC=C3
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InChi Key |
URFCJEUYXNAHFI-UHFFFAOYSA-N
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InChi Code |
InChI=1S/C15H12O4/c16-10-6-11(17)15-12(18)8-13(19-14(15)7-10)9-4-2-1-3-5-9/h1-7,13,16-17H,8H2
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Chemical Name |
5,7-dihydroxy-2-phenyl-2,3-dihydrochromen-4-one
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Synonyms |
DL-0108; DL0108; DL 0108; Pinocembrin
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HS Tariff Code |
2934.99.9001
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Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80: Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 :Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400  (Please use freshly prepared in vivo formulations for optimal results.) |
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Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
1 mM | 3.9024 mL | 19.5122 mL | 39.0244 mL | |
5 mM | 0.7805 mL | 3.9024 mL | 7.8049 mL | |
10 mM | 0.3902 mL | 1.9512 mL | 3.9024 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration: mg/mL;
Method for preparing DMSO stock solution: mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation::Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O,mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
Chemical structure of pinocembrin. Mol Neurobiol . 2016 Apr;53(3):1794-1801. td> |
Potential therapeutic applications of pinocembrin. In the central nervous system (CNS), pinocembrin is a drug candidate for ischemic stroke, but it also exerts protection against Alzheimer’s disease (AD) and Parkinson’s disease (PD). Mol Neurobiol . 2016 Apr;53(3):1794-1801. td> |
Chemical structure and natural sources of pinocembrin. Biomed Res Int . 2013:2013:379850. td> |