| Size | Price | Stock | Qty |
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| 50mg |
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| 100mg |
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| 250mg | |||
| Other Sizes |
| Targets |
Phenolphthalein glucuronide sodium targets β-glucuronidase, an enzyme that hydrolyzes glucuronide conjugates. β-Glucuronidase is involved in the metabolism of various endogenous and exogenous compounds. The compound serves as a substrate for this enzyme; upon enzymatic cleavage, phenolphthalein is released, which can be detected colorimetrically at alkaline pH. This property makes it a valuable tool for measuring β-glucuronidase activity in biological samples.
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| ln Vitro |
In vitro, Phenolphthalein glucuronide sodium is used as a substrate for β-glucuronidase in enzymatic assays. Upon cleavage by β-glucuronidase, free phenolphthalein is released, which develops a pink color at alkaline pH. This color change can be quantified spectrophotometrically to measure enzyme activity. The compound has been found to exhibit various biological activities, including anti-inflammatory, antioxidant, and anticancer properties.
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| ln Vivo |
Phenolphthalein glucuronide sodium does not have direct in vivo biological activity as a pharmacological agent. It is a substrate for β-glucuronidase used in biochemical assays. The compound is formed in the body through the glucuronidation of phenolphthalein. It is not used as a therapeutic agent.
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| Enzyme Assay |
In vitro enzymatic assays for Phenolphthalein glucuronide sodium measure β-glucuronidase activity. The substrate is incubated with the enzyme, and the released phenolphthalein is detected by adding an alkaline solution and measuring absorbance at 540-560 nm. These assays are used to measure β-glucuronidase activity in various biological samples.
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| Cell Assay |
Cell-based assays for Phenolphthalein glucuronide sodium are limited, as the compound is primarily used as a substrate in cell-free enzymatic assays. It may be used in cell culture supernatants to measure β-glucuronidase activity released from cells. However, specific protocols are not extensively documented.
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| Animal Protocol |
In vivo animal experiments are not applicable to Phenolphthalein glucuronide sodium as it is a research reagent, not a therapeutic agent. The compound is not intended for administration to animals. Its value is as a biochemical tool for in vitro studies.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to Phenolphthalein glucuronide sodium as it is a research reagent, not a therapeutic agent. The compound has a molecular formula of C26H21NaO10 and a molecular weight of 516.43. Solubility: H2O: soluble 100 mg/mL. Storage: appropriately.
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| Toxicity/Toxicokinetics |
Safety and toxicology data for Phenolphthalein glucuronide sodium are limited. As a biochemical reagent, it should be handled with appropriate safety precautions. The compound is for research use only and is not for human therapeutic use. No specific toxicity data are available.
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| Additional Infomation |
See also: Phenolphthalein glucuronide (note moved to).
Phenolphthalein glucuronide sodium has CAS number 6820-54-8, molecular formula C26H21NaO10, and molecular weight 516.43. Synonyms: phenolphthalein β-D-glucuronide sodium salt. It is a substrate for β-glucuronidase. Not for human use; for research purposes only. |
| Molecular Formula |
C26H21O10-.NA+
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|---|---|
| Molecular Weight |
516.42874
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| Exact Mass |
516.103
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| CAS # |
6820-54-8
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| Related CAS # |
15265-26-6 (Parent)
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| PubChem CID |
23708193
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| Appearance |
Typically exists as solid at room temperature
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| LogP |
0.84
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| Hydrogen Bond Donor Count |
4
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| Hydrogen Bond Acceptor Count |
10
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| Rotatable Bond Count |
5
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| Heavy Atom Count |
37
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| Complexity |
811
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| Defined Atom Stereocenter Count |
5
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| SMILES |
c1ccc2c(c1)C(=O)OC2(c3ccc(cc3)O)c4ccc(cc4)OC5[C@@H]([C@H]([C@@H]([C@H](O5)C(=O)[O-])O)O)O.[Na+]
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| InChi Key |
GBLJULUMBNYFAK-PDFLYTMFSA-M
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| InChi Code |
InChI=1S/C26H22O10.Na/c27-15-9-5-13(6-10-15)26(18-4-2-1-3-17(18)24(33)36-26)14-7-11-16(12-8-14)34-25-21(30)19(28)20(29)22(35-25)23(31)32;/h1-12,19-22,25,27-30H,(H,31,32);/q;+1/p-1/t19-,20-,21+,22-,25+,26?;/m0./s1
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| Chemical Name |
sodium;(2S,3S,4S,5R,6S)-3,4,5-trihydroxy-6-[4-[1-(4-hydroxyphenyl)-3-oxo-2-benzofuran-1-yl]phenoxy]oxane-2-carboxylate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 1.9364 mL | 9.6819 mL | 19.3637 mL | |
| 5 mM | 0.3873 mL | 1.9364 mL | 3.8727 mL | |
| 10 mM | 0.1936 mL | 0.9682 mL | 1.9364 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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