| Size | Price | Stock | Qty |
|---|---|---|---|
| 100mg |
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| 250mg |
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| 500mg |
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| Other Sizes |
| Targets |
Phenazine ethosulfate does not have a specific biological target as a drug. It is a redox mediator and electron acceptor used in various biochemical applications. Its redox properties allow it to participate in oxidation-reduction reactions, which are essential in metabolic processes. It is used as an electron acceptor in dye ligase assays and to detect nitric oxide reductase activity.
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| ln Vitro |
In vitro, Phenazine ethosulfate is used as a cationic dye and electron acceptor in biochemical assays. It is used in dye ligase assays and as an intermediate to detect the activity of nitric oxide reductase (Nors) in conjunction with ascorbic acid. The compound exhibits stability under a range of pH conditions, making it suitable for use in biological systems.
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| ln Vivo |
Phenazine ethosulfate does not have in vivo biological activity as a pharmacological agent. It is not intended for administration to animals or humans as a therapeutic. Its value is as a laboratory reagent for biochemical research. The compound is used in various in vitro applications.
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| Enzyme Assay |
In vitro biochemical assays using Phenazine ethosulfate include dye ligase assays and nitric oxide reductase activity assays. The compound serves as an electron acceptor in these assays. Its redox properties allow it to participate in oxidation-reduction reactions. The compound's fluorescence (Ex=390 nm, Em=530 nm) can be used for detection.
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| Cell Assay |
Cell-based assays for Phenazine ethosulfate are limited, as the compound is primarily used in cell-free biochemical assays. It may be used in cell-based assays as an electron acceptor, but specific protocols are not extensively documented.
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| Animal Protocol |
In vivo animal experiments are not applicable to Phenazine ethosulfate as it is a research reagent rather than a therapeutic agent. The compound is not intended for administration to animals. Its value is as a laboratory reagent for biochemical research.
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| ADME/Pharmacokinetics |
Pharmacokinetic properties are not applicable to Phenazine ethosulfate as it is a research reagent rather than a therapeutic agent. The compound has a molecular formula of C16H18N2O4S and a molecular weight of 334.39. Appearance: Yellow orange to orange brown powder. Solubility: water soluble. Storage: room temperature.
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| Toxicity/Toxicokinetics |
Safety and toxicology data for Phenazine ethosulfate are limited. As a chemical reagent, it should be handled with appropriate safety precautions. The compound is for research use only and is not for human therapeutic use. No specific toxicity data are available.
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| References |
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| Additional Infomation |
Phenazine ethosulfate has CAS number 10510-77-7, molecular formula C16H18N2O4S, and molecular weight 334.39. Synonyms: 5-ethylphenazinium ethyl sulfate. It is a water-soluble cationic phenazine dye and electron acceptor used in biochemical research. Purity: typically ≥95%. Not for human use; for research purposes only.
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| Molecular Formula |
C14H13N2+.C2H5O4S-
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|---|---|
| Molecular Weight |
334.39012
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| Exact Mass |
334.098
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| CAS # |
10510-77-7
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| PubChem CID |
82688
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| Appearance |
Yellow to orange solid powder
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| Melting Point |
190℃
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| LogP |
3.259
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| Hydrogen Bond Donor Count |
0
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| Hydrogen Bond Acceptor Count |
5
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| Rotatable Bond Count |
2
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| Heavy Atom Count |
23
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| Complexity |
323
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| Defined Atom Stereocenter Count |
0
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| InChi Key |
VDJKJPMLWJWQIH-UHFFFAOYSA-M
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| InChi Code |
InChI=1S/C14H13N2.C2H6O4S/c1-2-16-13-9-5-3-7-11(13)15-12-8-4-6-10-14(12)16;1-2-6-7(3,4)5/h3-10H,2H2,1H3;2H2,1H3,(H,3,4,5)/q+1;/p-1
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| Chemical Name |
5-ethylphenazin-5-ium;ethyl sulfate
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| HS Tariff Code |
2934.99.9001
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| Storage |
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition |
Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)
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| Solubility (In Vitro) |
DMSO : ~100 mg/mL (~299.05 mM)
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| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples.
Injection Formulations
Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline)(e.g. IP/IV/IM/SC) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). View More
Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] Oral Formulations
Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). View More
Oral Formulation 3: Dissolved in PEG400 (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 2.9905 mL | 14.9526 mL | 29.9052 mL | |
| 5 mM | 0.5981 mL | 2.9905 mL | 5.9810 mL | |
| 10 mM | 0.2991 mL | 1.4953 mL | 2.9905 mL |
*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.
Calculation results
Working concentration： mg/mL；
Method for preparing DMSO stock solution： mg drug pre-dissolved in μL DMSO (stock solution concentration mg/mL). Please contact us first if the concentration exceeds the DMSO solubility of the batch of drug.
Method for preparing in vivo formulation:：Take μL DMSO stock solution, next add μL PEG300, mix and clarify, next addμL Tween 80, mix and clarify, next add μL ddH2O，mix and clarify.
(1) Please be sure that the solution is clear before the addition of next solvent. Dissolution methods like vortex, ultrasound or warming and heat may be used to aid dissolving.
(2) Be sure to add the solvent(s) in order.
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